Pharmacokinetics and tissue distribution study of scoparone in rats by ultraperformance liquid-chromatography with tandem high-definition mass spectrometry

Yin, Quanwei; Sun, Hui; Zhang, Aihua; Wang, Xijun

doi:10.1016/j.fitote.2012.03.010

Cited by 37 publications

(25 citation statements)

References 19 publications

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“…Evidence shows that scoparone could yield potential benefits in the treatment and prevention for liver diseases, such as a potent inhibitor of hepatic fibrosis induced by carbon tetrachloride Yin et al, 2012;Zhang et al, 2011 and. All these results suggest that it may be a potent drug candidate for further study and up to now, its therapeutic mechanism has still been completely unclear.…”

Section: Discussionmentioning

confidence: 99%

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Proteomics Analysis of Hepatoprotective Effects for Scoparone Using MALDI-TOF/TOF Mass Spectrometry with Bioinformatics

Zhang

Sun

et al. 2013

OMICS: A Journal of Integrative Biology

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Scoparone is an active ingredient of Yinchenhao (Artemisia annua L.), a well-known Chinese medicinal plant, and has been utilized in prevention and therapy of liver damage. However, the molecular drug targets associated with the pharmacological effects of scoparone are largely unknown. In the present article, we extend the previous research on Yinchenhao through a study of its active ingredient and thus the putative targets of scoparone. We employed two-dimensional gel electrophoresis, and all proteins expressed were identified by MALDI-TOF/TOF MS and database research. Protein-interacting networks and pathways were also mapped and evaluated. The possible protein network associated with scoparone was constructed, and contribution of these proteins to the protective effect of scoparone against the carbon tetrachloride-induced acute liver injury in rats are discussed herein. Hepatoprotective effects of scoparone on liver injury in rats were associated with regulated expression of six proteins which were closely related in our protein-protein interaction network, and appear to be involved in antioxidation and signal transduction, energy production, immunity, metabolism, and chaperoning. These observations collectively provide new insights on the molecular mechanisms of scoparone action against hepatic damage in rats.

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Section: Discussionmentioning

confidence: 99%

“…Yinchenhao (Artemisia annua L.) is one of the most popular traditional Chinese medicinal plants for treatment of liver disorders Wang et al, 2013;Zhang et al, 2011). It can be used clinically to treat liver fibrosis, cholestasis, hepatitis C, and cholestatic diseases (Yin et al, 2012). Interestingly, scoparone (Fig.…”

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confidence: 99%

Proteomics Analysis of Hepatoprotective Effects for Scoparone Using MALDI-TOF/TOF Mass Spectrometry with Bioinformatics

Zhang

Sun

et al. 2013

OMICS: A Journal of Integrative Biology

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“…It found that D was distributed and eliminated rapidly. Tissue distribution showed the highest level was observed in liver, followed by the kidney and spleen; the lower level appeared in the muscle, thyroid, and adrenal [20]. It was not detected in the brain which indicated that D does not cross the blood-brain barrier after oral administration.…”

Section: Pharmacokinetic Studiesmentioning

confidence: 99%

Advancing Drug Discovery and Development from Active Constituents of Yinchenhao Tang, a Famous Traditional Chinese Medicine Formula

Zhang

Sun

Qiu

et al. 2013

Evidence-Based Complementary and Alternative Medicine

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Traditional Chinese medicine (TCM) formula has been playing a very important role in health protection and disease control for thousands of years. Guided by TCM syndrome theories, formula are designed to contain a combination of various kinds of crude drugs that, when combined, will achieve synergistic efficacy. However, the precise mechanism of synergistic action remains poorly understood. One example is a famous TCM formula Yinchenhao Tang (YCHT), whose efficacy in treating hepatic injury (HI) and Jaundice syndrome, has recently been well established as a case study. We also conducted a systematic analysis of synergistic effects of the principal compound using biochemistry, pharmacokinetics and systems biology, to explore the key molecular mechanisms. We had found that the three component (6,7-dimethylesculetin (D), geniposide (G), and rhein (R)) combination exerts a more robust synergistic effect than any one or two of the three individual compounds by hitting multiple targets. They can regulate molecular networks through activating both intrinsic and extrinsic pathways to synergistically cause intensified therapeutic effects. This paper provides an overview of the recent and potential developments of chemical fingerprinting coupled with systems biology advancing drug discovery towards more agile development of targeted combination therapies for the YCHT.

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“…UHPLC-MS pharmacokinetic studies of TCMs have been performed by many groups (Yang et al 2012; Li et al 2012; Qiu et al 2011;Yin et al 2012; Gan et al 2012; Xiong et al 2010; Wang, et al 2011; Sun et al 2012). A pharmaco-metabolomics study to explore the therapeutic basis and to clarify the mechanism of traditional Chinese medicine by UHPLC-MS has also been reported (Sun et al 2013; Zhang et al 2011).…”

Section: Introductionmentioning

confidence: 99%

Recent Advances in Ultra-High Performance Liquid Chromatography for the Analysis of Traditional Chinese Medicine

2014

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Traditional Chinese medicine has been widely used for the prevention and treatment of various diseases for thousands of years in China. Ultra-high performance liquid chromatography (UHPLC) is a relatively new technique offering new possibilities. This paper reviews recent developments in UHPLC in the separation and identification, fingerprinting, quantification, and metabolism of traditional Chinese medicine. Recently, the combination of UHPLC with MS has improved the efficiency of the analysis of these materials.

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Pharmacokinetics and tissue distribution study of scoparone in rats by ultraperformance liquid-chromatography with tandem high-definition mass spectrometry

Cited by 37 publications

References 19 publications

Proteomics Analysis of Hepatoprotective Effects for Scoparone Using MALDI-TOF/TOF Mass Spectrometry with Bioinformatics

Proteomics Analysis of Hepatoprotective Effects for Scoparone Using MALDI-TOF/TOF Mass Spectrometry with Bioinformatics

Advancing Drug Discovery and Development from Active Constituents of Yinchenhao Tang, a Famous Traditional Chinese Medicine Formula

Recent Advances in Ultra-High Performance Liquid Chromatography for the Analysis of Traditional Chinese Medicine

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