Pharmacological Ascorbate as an Adjuvant for Enhancing Radiation-Chemotherapy Responses in Gastric Adenocarcinoma

O’Leary, Brianne R.; Houwen, Frederick K.; Johnson, Chase L.; Allen, Bryan G.; Mezhir, James J.; Berg, Daniel J.; Cullen, Joseph J.; Spitz, Douglas R.

doi:10.1667/rr14978.1

Cited by 33 publications

(38 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These same genes have also been nominated as candidate genes for alcohol dependence in a human genetic association study (Zuo et al, 2011 ). We further confirmed that Ptp4a1 is a candidate gene in stress and alcohol responses (Baker et al, 2017 ) by using differentially expressed (DE) genes and QTL analysis in BXD recombinant inbred (RI) strains. In addition, we identified a sequence variant in a circadian rhythm gene, Per3 , and associated it with stress and alcohol responses by integrating expression QTL (eQTL) and phenotype QTL analyses (Wang et al, 2012 ).…”

Section: Introductionsupporting

confidence: 75%

“…In addition, G-protein alpha ( Gna1 , Gnal2 , Gnb1 and Gng2 ) and its regulators ( Rgs10 and Rgs14 ) are included in this subnetwork. The involvement of these genes in both alcohol and stress responses is well known (Baker et al, 2017 ).…”

Section: Resultsmentioning

confidence: 99%

“…Hippocampal dissection was conducted and hippocampal RNA was isolated according to manufacturer’s protocol using RNA STAT-60. Gene expression for all groups was examined by microarray analysis as previously described (Wang et al, 2012 ; Baker et al, 2017 ). Hippocampal gene expression was analyzed using Illumina v6.1 microarrays, according to the manufacturer’s protocol 1 .…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Integrating Genetic and Gene Co-expression Analysis Identifies Gene Networks Involved in Alcohol and Stress Responses

Luo¹,

Xu²,

Cao³

et al. 2018

Front. Mol. Neurosci.

View full text Add to dashboard Cite

Although the link between stress and alcohol is well recognized, the underlying mechanisms of how they interplay at the molecular level remain unclear. The purpose of this study is to identify molecular networks underlying the effects of alcohol and stress responses, as well as their interaction on anxiety behaviors in the hippocampus of mice using a systems genetics approach. Here, we applied a gene co-expression network approach to transcriptomes of 41 BXD mouse strains under four conditions: stress, alcohol, stress-induced alcohol and control. The co-expression analysis identified 14 modules and characterized four expression patterns across the four conditions. The four expression patterns include up-regulation in no restraint stress and given an ethanol injection (NOE) but restoration in restraint stress followed by an ethanol injection (RSE; pattern 1), down-regulation in NOE but rescue in RSE (pattern 2), up-regulation in both restraint stress followed by a saline injection (RSS) and NOE, and further amplification in RSE (pattern 3), and up-regulation in RSS but reduction in both NOE and RSE (pattern 4). We further identified four functional subnetworks by superimposing protein-protein interactions (PPIs) to the 14 co-expression modules, including γ-aminobutyric acid receptor (GABA) signaling, glutamate signaling, neuropeptide signaling, cAMP-dependent signaling. We further performed module specificity analysis to identify modules that are specific to stress, alcohol, or stress-induced alcohol responses. Finally, we conducted causality analysis to link genetic variation to these identified modules, and anxiety behaviors after stress and alcohol treatments. This study underscores the importance of integrative analysis and offers new insights into the molecular networks underlying stress and alcohol responses.

show abstract

Section: Introductionsupporting

confidence: 75%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Integrating Genetic and Gene Co-expression Analysis Identifies Gene Networks Involved in Alcohol and Stress Responses

Luo¹,

Xu²,

Cao³

et al. 2018

Front. Mol. Neurosci.

View full text Add to dashboard Cite

show abstract

“…In reality, against the advances in the classic tumor chemotherapy, the prediction chance of the advanced gastric cancer in patients is yet very low. Agent 5-FU, being anti-neoplastic in nature, is the first-line chemotherapy having a wide range of activity in gastro-intestinal tumors; however, like the majority of tumor medicines, its major disadvantage is the high level of toxicity and the low rate of therapeutic effect (O'Leary et al, 2018;Shah and Schwartz, 2001). As a result, it is urgently required that new therapeutic modalities be introduced for the gastric tumor.…”

Section: Discussionmentioning

confidence: 99%

Evaluating of Induction of Apoptosis by Cornus mass L. Extract in the Gastric Carcinoma Cell Line (AGS)

Hosseini

Karimabad

Hajizadeh

et al. 2019

Asian Pac J Cancer Prev

View full text Add to dashboard Cite

Aim and objectives: Natural products and derivatives of medicinal vegetation can play an important role to the cure tumor. The Present study was focused to determine the effect of Cornus mass L. extract on the induction of apoptosis in AGS gastric carcinoma cell line in compared to L929 cells. Methods: In this experimental study, AGS and L929 cells were cultured and treated with different concentrations (0-10 mg/ml) of Cornus mass L. extract for 48 and 72 hours. Cell proliferation was assessed by MTT assay. The optical density of the colored solution was quantified at 570 nm wavelengths by an ELISA Reader. Making use of the apoptosis detection kit of Annexin V-FITC, PI and double staining with Annexin V-FITC were carried out for flow cytometry investigations. Data were analyzed by ANOVA. Variations with a P-value less than 0.05 were considered significant. Results: shows a noticeable deviation among various concentrations of extract when cells were treated for 48, 72 h declined cell viability in AGS cell line in comparison L929 cell lines in a dose and time-dependent manner (P < 0.05). This extract also displayed approximately several-fold increased anti-cancer potency in AGS compared to L929 cells. The IC 50 value in AGS cells (evaluated after 48,72h) of the extract against AGS cells was 5/44, 2/44 mg/ml (p≤0.05). The analysis results of flow cytometry indicated that apoptosis was induced by the extract in AGS cells treated, compared with L929 cells. Conclusion: Each of our results implicates the reality that Cornus mass L. extract acts as a novel, potent inhibitor of cancer proliferation in in vitro. This may result in developing a promising therapeutic agent for the treatment of indole-sensitive cancers.

show abstract

“…Experiments performed in vitro to test compatibility with other anti-carcinogenic substances revealed that AA can have a synergistic effect with some of them (Espey et al, 2011;Ma et al, 2014;Hatem et al, 2018;O'Leary et al, 2018;Graczyk-Jarzynka et al, 2019). For instance, Gemcitabine in combination with AA (Espey et al, 2011) have a synergistic cytotoxic effect in eight pancreatic cancer cell lines, which is mediated by the pro-oxidant effect of ascorbate, again with an increase in the production of H 2 O 2 .…”

Section: Effect Of Pharmacologic Vitamin C In Cancer Cellsmentioning

confidence: 99%

Therapeutic Use of Vitamin C in Cancer: Physiological Considerations

et al. 2020

View full text Add to dashboard Cite

Since the early studies of William J. McCormick in the 1950s, vitamin C has been proposed as a candidate for the treatment of cancer. A number of reports have shown that pharmacological concentrations of vitamin C selectively kill cancer cells in vitro and decrease the growth rates of a number of human tumor xenografts in immunodeficient mice. However, up to the date there is still doubt regarding this possible therapeutic role of vitamin C in cancer, mainly because high dose administration in cancer patients has not showed a clear antitumor activity. These apparent controversial findings highlight the fact that we lack information on the interactions that occurs between cancer cells and vitamin C, and if these transformed cells can uptake, metabolize and compartmentalize vitamin C like normal human cells do. The role of SVCTs and GLUTs transporters, which uptake the reduced form and the oxidized form of vitamin C, respectively, has been recently highlighted in the context of cancer showing that the relationship between vitamin C and cancer might be more complex than previously thought. In this review, we analyze the state of art of the effect of vitamin C on cancer cells in vitro and in vivo, and relate it to the capacity of cancer cells in acquiring, metabolize and compartmentalize this nutrient, with its implications on the potential therapeutic role of vitamin C in cancer.

show abstract

Pharmacological Ascorbate as an Adjuvant for Enhancing Radiation-Chemotherapy Responses in Gastric Adenocarcinoma

Cited by 33 publications

References 39 publications

Integrating Genetic and Gene Co-expression Analysis Identifies Gene Networks Involved in Alcohol and Stress Responses

Integrating Genetic and Gene Co-expression Analysis Identifies Gene Networks Involved in Alcohol and Stress Responses

Evaluating of Induction of Apoptosis by Cornus mass L. Extract in the Gastric Carcinoma Cell Line (AGS)

Therapeutic Use of Vitamin C in Cancer: Physiological Considerations

Contact Info

Product

Resources

About