Phase behavior of selected artificial lipids

Dobner, Bodo; Brezesinski, Gerald

doi:10.1016/j.cocis.2014.01.003

Cited by 13 publications

(8 citation statements)

References 63 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Apparently, interdigitation of the diC14-amidine gel phase bilayers does not depend exclusively on the interactions between charged headgroups, but also on the architecture of the lipid backbone. Consistently, interdigitation due to increased intramolecular chain separation is also observed in gel phase bilayers of 1,3-DPPC, a synthetic isomer of zwitterionic DPPC (Serrallach et al 1983;Zumbuehl et al 2014).…”

Section: Different Gel Bilayer Structures: Interdigitationsupporting

confidence: 57%

Structural insights on biologically relevant cationic membranes by ESR spectroscopy

et al. 2017

View full text Add to dashboard Cite

Cationic bilayers have been used as models to study membrane fusion, templates for polymerization and deposition of materials, carriers of nucleic acids and hydrophobic drugs, microbicidal agents and vaccine adjuvants. The versatility of these membranes depends on their structure. Electron spin resonance (ESR) spectroscopy is a powerful technique that employs hydrophobic spin labels to probe membrane structure and packing. The focus of this review is the extensive structural characterization of cationic membranes prepared with dioctadecyldimethylammonium bromide or diC14-amidine to illustrate how ESR spectroscopy can provide important structural information on bilayer thermotropic behavior, gel and fluid phases, phase coexistence, presence of bilayer interdigitation, membrane fusion and interactions with other biologically relevant molecules.

show abstract

Section: Different Gel Bilayer Structures: Interdigitationsupporting

confidence: 57%

Structural insights on biologically relevant cationic membranes by ESR spectroscopy

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Such interdigitation is described for 1,3-alkylated phospholipids, which also exhibit a larger distance between the alkyl chains. 38,39 Zumbuehl and co-workers also described a lipid (1,3-dipalmitamidopropan-2phosphocholine) that shows an LE/LC phase transition above 35 mN m À1 at 25 1C as a Langmuir monolayer and an interdigitated lamellar gel phase in the bulk (3D system). 31,39 Also, the small bilayer thickness of around 47 Å for both lipids indicates an interdigitated gel phase.…”

Section: Characterization Of the Aggregation Behaviour Of Pure Cationmentioning

confidence: 99%

Structures of malonic acid diamide/phospholipid composites and their lipoplexes

et al. 2016

Self Cite

View full text Add to dashboard Cite

As a continuation of previous work, the self-assembly process of cationic lipid formulations in the presence and absence of DNA was investigated with respect to binary lipid mixtures suitable as polynucleotide carrier systems. The lipid blends consist of one malonic-acid-based cationic lipid with a varying alkyl chain pattern, either N-{6-amino-1-[N-(9Z)-octadec-9-enylamino]-1-oxohexan-(2S)-2-yl}-N'-{2-[N,N-bis(2-aminoethyl)amino]ethyl}-2-hexadecylpropandiamide () or N-[6-amino-1-oxo-1-(N-tetradecylamino)hexan-(2S)-2-yl]-N'-{2-[N,N-bis(2-aminoethyl)amino]ethyl}-2-hexadecylpropandiamide (), and one neutral co-lipid, either 1,2-di-[(9Z)-octadec-9-enoyl]-sn-glycero-3-phosphocholine (DOPE) or 1,2-di-(hexadecanoyl)-sn-glycero-3-phosphocholine (DPPC). Although the cationic lipids exhibit only slight differences in their structure, the DNA transfer efficiency varies drastically. Therefore, self-assembly was studied in 3D systems by small- and wide-angle X-ray scattering (SAXS and WAXS) and transmission electron microscopy (TEM) as well as in 2D systems by infrared reflection-absorption spectroscopy (IRRAS) on Langmuir films. The investigated lipid mixtures show quite different self-assembly in the absence of DNA, with varying structures from vesicles (/DOPE; /DOPE) and tubes (/DOPE) to discoid structures (/DPPC; /DPPC). Twisted ribbons and sheets, which were stabilized due to hydrogen-bond networks, were found in all investigated lipid mixtures in the absence of DNA. The addition of DNA leads to the formation of lamellar lipoplexes for all the investigated lipid compositions. The lipoplexes differ in crucial parameters, such as the lamellar repeat distance and the spacing between the DNA strands, indicating differences in the binding strength between DNA and the lipid composition. The formation of associates with an ideal charge density might emerge as a key parameter for efficient DNA transfer. Furthermore, the structures observed for the different lipid compositions in the absence of DNA prepare the way for other applications besides gene therapy.

show abstract

“…A sufficient asymmetry in the chain length tends to induce chain interdigitation in bilayers. 6,12 The malonic acid/lysine backbone of TT10, TH10, OT10, OH10 and OO10 is biocompatible. Due to this backbone the lipids exhibit a peptide-mimicking character.…”

Section: Introductionmentioning

confidence: 99%

Lysine-based amino-functionalized lipids for gene transfection: 3D phase behaviour and transfection performance

Tassler

Pawlowska

Janich

et al. 2018

Phys. Chem. Chem. Phys.

Self Cite

View full text Add to dashboard Cite

Based on previous work, the influence of the chain composition on the physical-chemical properties of five new transfection lipids (TH10, TT10, OH10, OT10 and OO10) containing the same lysine-based head group has been investigated in aqueous dispersions. For this purpose, the chain composition has been gradually varied from saturated tetradecyl (T, C14:0) and hexadecyl (H, C16:0) chains to longer but unsaturated oleyl (O, C18:1) chains with double bonds in the cis configuration. In this work, the lipid dispersions have been investigated in the absence and presence of the helper lipid DOPE and calf thymus DNA by small-angle and wide-angle X-ray scattering (SAXS/WAXS) supplemented by differential scanning calorimetry (DSC), attenuated total reflection Fourier-transform infrared spectroscopy (ATR-FTIR) and Fourier-transform Raman spectroscopy (FTRS). Lamellar and inverted hexagonal mesophases have been observed in single-component systems. In the binary mixtures, the aggregation behaviour changes with an increasing amount of DOPE from lamellar to cubic. The lipid mixtures with DNA show a panoply of mesophases. Interestingly, TT10 and OT10 form cubic lipoplexes, whereas OO10 complexes the DNA sandwich-like between lipid bilayers in a lamellar lipoplex. Surprisingly, the latter is the most effective lipoplex.

show abstract

Phase behavior of selected artificial lipids

Cited by 13 publications

References 63 publications

Structural insights on biologically relevant cationic membranes by ESR spectroscopy

Structural insights on biologically relevant cationic membranes by ESR spectroscopy

Structures of malonic acid diamide/phospholipid composites and their lipoplexes

Lysine-based amino-functionalized lipids for gene transfection: 3D phase behaviour and transfection performance

Contact Info

Product

Resources

About