Phase contrast X-ray synchrotron imaging for assessing external and internal morphology of Rhodnius prolixus

Almeida, André Pereira de; Soares, José Marques; Meneses, Anderson Alvarenga de Moura; Cardoso, Simone C.; Braz, Delson; Garcia, Elói S.; Gonzalez, Marcelo S.; Barroso, R.C.

doi:10.1016/j.apradiso.2011.11.028

Cited by 12 publications

(8 citation statements)

References 24 publications

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“…It has made possible access to information on organs and structure of insects [22]. Gao et al studied human liver cancer and was able to visualize the guinea pig’s cochlea [23].…”

Section: Introductionmentioning

confidence: 99%

Three-dimensional reconstruction of blood vessels in the rabbit eye by X-ray phase contrast imaging

et al. 2013

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BackgroundA clear understanding of the blood vessels in the eye is helpful in the diagnosis and treatment of ophthalmic diseases, such as glaucoma. Conventional techniques such as micro-CT imaging and histology are not sufficiently accurate to identify the vessels in the eye, because their diameter is just a few microns. The newly developed medical imaging technology, X-ray phase-contrast imaging (XPCI), is able to distinguish the structure of the vessels in the eye. In this study, XPCI was used to identify the internal structure of the blood vessels in the eye.MethodsAfter injection with barium sulfate via the ear border artery, an anesthetized rabbit was killed and its eye was fixed in vitro in 10% formalin solution. We acquired images using XPCI at the Shanghai Synchrotron Radiation Facility. The datasets were converted into slices by filtered back-projection (FBP). An angiographic score was obtained as a parameter to quantify the density of the blood vessels. A three-dimensional (3D) model of the blood vessels was then established using Amira 5.2 software.ResultsWith XPCI, blood vessels in the rabbit eye as small as 18 μm in diameter and a sixth of the long posterior ciliary artery could be clearly distinguished. In the 3D model, we obtained the level 4 branch structure of vessels in the fundus. The diameters of the arteria centralis retinae and its branches are about 200 μm, 110 μm, 95 μm, 80 μm and 40 μm. The diameters of the circulus arteriosus iridis major and its branches are about 210 μm, 70 μm and 30 μm. Analysis of vessel density using the angiographic score showed that the blood vessels had maximum density in the fundus and minimum density in the area anterior to the equator (scores 0.27 ± 0.029 and 0.16 ± 0.032, respectively). We performed quantitative angiographic analysis of the blood vessels to further investigate the density of the vessels.ConclusionsXPCI provided a feasible means to determine the structure of the blood vessels in the eye. We were able to determine the diameters and morphological characteristics of the vessels from both 2D images and the 3D model. By analyzing the images, we obtained measurements of the density distribution of the microvasculature, and this approach may provide valuable reference information prior to glaucoma filtration surgery.

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“…It has made possible access to information on organs and structure of insects [22]. Gao et al studied human liver cancer and was able to visualize the guinea pig’s cochlea [23].…”

Section: Introductionmentioning

confidence: 99%

Three-dimensional reconstruction of blood vessels in the rabbit eye by X-ray phase contrast imaging

et al. 2013

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“…Until now, internal and external features of Rhodnius prolixus head in a solution containing 1% glutaraldehyde and 5% sucrose in 0.1M cacodylate buffer were studied using synchrotron microtomography at the SYRMEP (SYnchrotron Radiation for MEdical Physics) beamline at Elettra Synchrotron facility, Trieste, Italy, on monochromatic [4][5][6] and polychromatic configuration [7][8].…”

Section: Resultsmentioning

confidence: 99%

“…Microcomputed tomography (microCT) provides an excellent tool for 3D reconstruction of Rhodnius prolixus [4][5][6][7][8], allowing virtually sectioning of the samples in any direction, with clearer visualizations, in different depths of the whole head, with high resolution and also keeping their natural state without elaborate and time-consuming dissection or histology. Although this approach can produce finely resolved images of internal morphology of insects [9][10][11], the improvement of the contrast between the low density tissues of the insect is somehow necessary so that structures can be better distinguished.…”

Section: Introductionmentioning

confidence: 99%

Effects of different fixation methods on the study of Rhodnius prolixus head using 3D microCT imaging

Sena¹,

Nogueira²,

Almeida³

et al. 2016

AIP Conference Proceedings

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Abstract. Chagas' disease, also called American trypanosomiasis, is a human tropical disease, which is endemic in large areas of South and Central America. Rhodnius prolixus is the most important insect vector of Trypanosoma cruzi, the etiologic agent of Chagas' disease. Studies have shown that microcomputed tomography (microCT) provides an excellent tool for 3D reconstruction of Rhodnius prolixus head. Although this approach can produce finely resolved images of internal morphology of insects, the improvement of the contrast between the low density tissues of the insect is somehow necessary so that structures can be better distinguished. An appropriate contrast staining and fixative methodology can produce high-quality images of soft tissues enhancing the contrast of the reconstructed images from microCT. In this work, different fixative methods were used to improve 3D imaging visualization of internal structures of Rhodnius prolixus using microCT. All specimens were imaged at Brazilian Synchrotron Laboratory (LNLS) . The fixatives used were gluteroldehyde, Bouin's fluid and Bouin's alcoholic. The results showed relevant differences in the images obtained with different fixatives. This work revealed that it is possible to improve contrast and visibility of details of Rhodnius prolixus and other insects as well, by the appropriate use of fixative and staining methods.

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“…In 2012 Almeida et al 11 . started the studies with Rhodnius prolixus and synchrotron microtomography using propagation phase contrast technique, a monochromatic beam and phase retrieval, providing excellent images of the insect’ morphology.…”

Section: Introductionmentioning

confidence: 99%

“…In 2012 Almeida et al 11 started the studies with Rhodnius prolixus and synchrotron microtomography using propagation phase contrast technique, a monochromatic beam and phase retrieval, providing excellent images of the insect' morphology. After 2014, the next works about Rhodnius prolixus and synchrotron microtomography were performed using the propagation phase contrast technique with a polychromatic beam, to decrease the measurement time and improve the resolution.…”

Section: Introductionmentioning

confidence: 99%

Investigation of Rhodnius prolixus head anatomy using microtomography

Sena

Silva

Nogueira

et al. 2023

Journal of Microscopy

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In the last years, microtomography has proved to be a powerful technique on insects' studies, allowing a detailed view of the structures' internal with a high resolution. One of the most important advantages about the use of microtomography in these studies is the fact that the dissection is not necessary, which decreases considerably the number of samples used on the insects' research. Some insects are used constantly in studies about morphology, metamorphosis, and reproduction, because they work as a model for others, and Rhodnius prolixus is one of the most studied in this group. This insect is also one of the main insect vectors of Chagas disease that kills around 12,000 people every year in Latin America. Some studies using laboratory microtomography conventional scanners combining with the correct staining methods have proved that it could be a powerful tool in biological research, allowing the visualisation of low-density tissues. The main goal of the present work was to use staining protocols to study Rhodnius prolixus with laboratory microtomography conventional scanners. The experiments were carried out at the imaging lab in the Theoretical Biology Department, University of Vienna, using an Xradia MicroXCT and at the University of Oslo, using a Skyscan 2211.

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Phase contrast X-ray synchrotron imaging for assessing external and internal morphology of Rhodnius prolixus

Cited by 12 publications

References 24 publications

Three-dimensional reconstruction of blood vessels in the rabbit eye by X-ray phase contrast imaging

Three-dimensional reconstruction of blood vessels in the rabbit eye by X-ray phase contrast imaging

Effects of different fixation methods on the study of Rhodnius prolixus head using 3D microCT imaging

Investigation of Rhodnius prolixus head anatomy using microtomography

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