Phase i imaging study of Hodgkin's disease and non-hodgkin's lymphomas with radiolabeled antibody to eosinophil peroxidase

Samoszuk, Michael; Anderson, A. L.; Wang, F.; Braunstein, Philip; Majmundar, Haresh; Ravel, Jyoti; Ramzi, Eiman; Slater, Lewis M.

doi:10.1007/bf02789477

Cited by 3 publications

(5 citation statements)

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“…The monoclonal antibody directed against EPO that we used in this study has already been radiolabeled and administered to a variety of patients. 5 These studies have clearly shown that the antibody localizes only to sites of active eosinophil degranulation , such as those in certain tumors and allergic nasal polyps. Although eosinophil degranulation is not a specific property of endometriosis or cancer, it may well prove to be a useful marker for tissue remodeling that is associated with a variety of diseases.…”

Section: Discussionmentioning

confidence: 99%

“…3,5,7 The intense, localized, red-brown staining of EPO and eosinophils was readily distinguishable from hemosiderin-laden macrophages, which had a granular, olive brown appearance ( Figure 1G) that persisted even in the absence of primary antibody.…”

Section: Epo Expression In Endometriosis and Normal Endometriamentioning

confidence: 99%

“…3 Its derivation, origin, and other properties are described in detail in previous publications. 3,5 Biotinylated horse anti-mouse second antibody (50 l of a 1:200 dilution of stock) was added and incubated for 30 minutes under humid conditions. Horse serum (HS) from Vectastain (Vector Labs, Burlingame, CA) was the blocking agent.…”

Section: Immunohistochemistrymentioning

confidence: 99%

“…4 Degranulating eosinophils have been identified in many lymphomas, breast cancers, and in most cases of endometrial cancer that were studied. [5][6][7] In normal endometrium, eosinophils accumulate just before and during menstruation. 8 This process is regulated, at least in part, by estradiol, 9,10 which controls the production of an eosinophil chemoattractant factor in rat.…”

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confidence: 99%

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Degranulating Eosinophils in Human Endometriosis

Blumenthal

Samoszuk

Taylor

et al. 2000

The American Journal of Pathology

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Degranulating eosinophils have been described in most endometrial cancers. We hypothesized that endometriosis (ectopic, nonneoplastic endometrial tissue) would be an appropriate model system for determining whether eosinophil degranulation is part of a specific immune response to endometrial cancer or if it is related to the more general phenomenon of tissue remodeling (wound healing) that is common to both disorders. To test this hypothesis, we performed immunohistochemistry and Western blotting to evaluate the presence of eosinophil peroxidase (a marker of eosinophil degranulation) in normal endometrium (n = 20) and endometriosis samples (n = 24) and to define the coexpression of three eosinophil chemoattractants: interleukin-5 (IL-5), eotaxin, and regulated on activator-normal T cell expressed and secreted (RANTES). There was focally intense deposition of eosinophil peroxidase in the fibrotic connective tissue and blood vessels of 21 of 24 human endometriosis specimens; two samples showed weak staining, and only one tissue was negative for eosinophil degranulation. None of the 10 normal proliferative endometrial specimens had evidence of eosinophil degranulation, and four of 10 secretory tissues stained only weakly for eosinophil peroxidase. The presence of degranulating eosinophils was also associated with the presence of eotaxin and IL-5 in some samples and with RANTES in others. We conclude that the abundant presence of degranulating eosinophils in the fibrous regions of endometriosis supports the interpretation that eosinophils are involved in general tissue remodeling and wound healing rather than a tissue-directed immune response.

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Section: Discussionmentioning

confidence: 99%

Section: Epo Expression In Endometriosis and Normal Endometriamentioning

confidence: 99%

Section: Immunohistochemistrymentioning

confidence: 99%

mentioning

confidence: 99%

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Degranulating Eosinophils in Human Endometriosis

Blumenthal

Samoszuk

Taylor

et al. 2000

The American Journal of Pathology

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“…These proteins were consequently not further validated by immunohistochemistry. For instance, hemopexin protein was found elevated in the serum of patients with HL [ 29 ]; eosinophil peroxidase has already been used for radio-immunodetection of Hodgkin's disease [ 30 ]; coagulation factor XIII was found to be expressed by macrophages and involved in the stabilization of fibrin deposits in the tumour stroma of HL samples [ 31 ]; and nucleoside diphosphate kinase A expression was found to be a prognostic factor for classical HL [ 32 ].…”

Section: Discussionmentioning

confidence: 99%

Identification of stromal proteins overexpressed in nodular sclerosis Hodgkin lymphoma

et al. 2011

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Hodgkin lymphoma (HL) represents a category of lymphoid neoplasms with unique features, notably the usual scarcity of tumour cells in involved tissues. The most common subtype of classical HL, nodular sclerosis HL, characteristically comprises abundant fibrous tissue stroma. Little information is available about the protein composition of the stromal environment from HL. Moreover, the identification of valid protein targets, specifically and abundantly expressed in HL, would be of utmost importance for targeted therapies and imaging, yet the biomarkers must necessarily be accessible from the bloodstream. To characterize HL stroma and to identify potentially accessible proteins, we used a chemical proteomic approach, consisting in the labelling of accessible proteins and their subsequent purification and identification by mass spectrometry. We performed an analysis of potentially accessible proteins in lymph node biopsies from HL and reactive lymphoid tissues, and in total, more than 1400 proteins were identified in 7 samples. We have identified several extracellular matrix proteins overexpressed in HL, such as versican, fibulin-1, periostin, and other proteins such as S100-A8. These proteins were validated by immunohistochemistry on a larger series of biopsy samples, and bear the potential to become targets for antibody-based anti-cancer therapies.

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Radiopharmaceuticals and Medical Imaging: Development of Radioimmumoconjugates for Radioimmunoimaging and Radioimmunotherapy of Malignant Diseases

Niu

Chen

2011

Pharmaceutical Sciences Encyclopedia

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Radioimmunopharmaceuticals or radioimmunoconjugates (RICs) are using antibody (Ab) or antibody derivatives as targeting carriers, taking advantage of the specific binding between antibody and antigen. In this chapter, we will discuss several aspects of RIC development and their application in radioimmunoimaging (RII) and radioimmunotherapy (RIT) for malignant diseases, including lymphoma, prostate cancer, colon cancer, and breast and ovarian cancers.

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Phase i imaging study of Hodgkin's disease and non-hodgkin's lymphomas with radiolabeled antibody to eosinophil peroxidase

Cited by 3 publications

References 1 publication

Degranulating Eosinophils in Human Endometriosis

Degranulating Eosinophils in Human Endometriosis

Identification of stromal proteins overexpressed in nodular sclerosis Hodgkin lymphoma

Radiopharmaceuticals and Medical Imaging: Development of Radioimmumoconjugates for Radioimmunoimaging and Radioimmunotherapy of Malignant Diseases

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