Phase separation dynamics in deformable droplets

Gsell, Simon; Merkel, Matthias

doi:10.1039/d1sm01647d

Cited by 5 publications

(4 citation statements)

References 62 publications

(112 reference statements)

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“…S12 in ESI and Supporting Video 4 † ). 54 This phenomenon indicates that the occurrence of phase separation is independent of the initial shapes of the droplets.…”

Section: Resultsmentioning

confidence: 93%

Controlled evaporation-induced phase separation of droplets containing nanogels and salt molecules

2022

RSC Adv.

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“…S12 in ESI and Supporting Video 4 † ). 54 This phenomenon indicates that the occurrence of phase separation is independent of the initial shapes of the droplets.…”

Section: Resultsmentioning

confidence: 93%

Controlled evaporation-induced phase separation of droplets containing nanogels and salt molecules

2022

RSC Adv.

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“…First, much of the past work on cellular unmixing focused on already differentiated cells [47]. However, if all cells kept their initial differentiation state over time, flows driven by differential tissue tensions may at some point cease [46]. However, the cells in our system are in the process of differentiating, and the observed recirculating flows are maintained over several hours, which makes us wonder whether the flows might be maintained through patterned cell differentiation.…”

Section: Discussionmentioning

confidence: 99%

“…In contrast, our work shows for the first time that differential tissue surface tensions can drive long-range hydrodynamic flows that are maintained over a long time ( > ∼ 10 hours). In previous work, some of us have shown that such long-range flows can be important to substantially accelerate cellular unmixing [46].…”

Section: Discussionmentioning

confidence: 99%

Marangoni-like tissue flows enhance symmetry breaking of embryonic organoids

Gsell,

Tlili,

Merkel

et al. 2023

Preprint

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During early development of multi-cellular animals, cells self-organize to set up the body axes, such as the primary head-to-tail axis, based on which the later body plan is defined. Several signaling pathways are known to control body axis formation. Here, we show, however, that tissue mechanics plays an important role during this process. We focus on the emergence of a primary axis in initially spherical aggregates of mouse embryonic stem cells, which mirrors events in the early mouse embryo. These aggregates break rotational symmetry to establish an axial organization with domains of different expression profiles, e.g. of the transcription factor T/Bra and the adhesion molecule E-cadherin. Combining quantitative microscopy and physical modeling, we identify largescale tissue flows with a recirculation component and demonstrate that they significantly contribute to symmetry breaking. We show that the recirculating flows are explained by a difference in tissue surface tension across domains, akin to Marangoni flows, which we further confirm by aggregate fusion experiments. Our work highlights that body axis formation is not only driven by biochemical processes, but that it can also be amplified by tissue flows. We expect this type of amplification to operate in many other organoid and in-vivo systems.

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“…Besides theoretical frameworks, [211,212] this consideration indicates the desperate need for continuum modeling that accounts for thermodynamic parameters while calculating hydrodynamics to bridge the gap between the individual sequences and macroscopic dynamic behaviors of DNA droplets. [214,215] Such numerical modeling could extract experimentally unachievable quantitative data as well as high-resolution visualization of dynamically restructuring DNA droplets in response to stimuli.…”

Section: Beyond Coarse-grained Modelingmentioning

confidence: 99%

DNA Droplets: Intelligent, Dynamic Fluid

et al. 2022

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Figure 1. Overview of DNA droplets as a hybrid from DNA nanotechnology and liquid-liquid phase separation (LLPS) studies. A) DNA droplets, micro-scale condensates of DNA nanostructures (DNA motifs) self-assembled via sticky ends (SEs). A Y-shaped DNA motif (Y-motif) is a branched nanostructure of three single-stranded DNAs (ssDNAs) hybridized to form the branching stems. At the end of each branch, a single-stranded SE protrudes. Two basic features are highlighted: (i) Programmable interactions. DNA droplets favor sequence-specifically selective interactions as encoded in the SE sequences. (ii) Programmability in mechanical properties.The number of the SEs in a single DNA motif, serving as the valency, can be designed to determine the macroscopic rheological properties, including diffusion coefficient and viscoelastic behavior. Adapted with permission. [29]

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Phase separation dynamics in deformable droplets

Abstract: Phase separation can drive spatial organization of multicomponent mixtures. For instance in developing animal embryos, effective phase separation descriptions have been used to account for the spatial organization of different...

Cited by 5 publications

References 62 publications

Controlled evaporation-induced phase separation of droplets containing nanogels and salt molecules

Controlled evaporation-induced phase separation of droplets containing nanogels and salt molecules

Marangoni-like tissue flows enhance symmetry breaking of embryonic organoids

DNA Droplets: Intelligent, Dynamic Fluid

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