SummaryThe channel plays a significant role as the multipath in Multiple Input Multiple Output‐Orthogonal Frequency Division Multiplexing (MIMO‐OFDM) systems. The precoding‐based channel estimation algorithms estimate the channel capacity at the receiver node and they provide efficient channel estimates under worst case channel scenarios. To estimate the channel capacity in the mobile node, semi‐blind scenario is a crucial task in Multi User (MU) MIMO systems. Therefore, a semi blind sparse channel estimation algorithm is demonstrated in the MU‐MIMO system to provide better performance. In the transmitter side, the signal modulation is performed with the help of Quadrature Phase Shift Keying (QPSK), and the modulated signal is given to Pulse Shaping Algorithm (PSA) to reduce the inter‐carrier interference as well as inter symbol interference. At each transmitter, the Inverse Fast Fourier Transforms (IFFT) technique is used for mapping the symbols and then the mapped signal is send to the receiver. In the receiver side, the FFT, pulse reshaping, and QPSK demodulation blocks are serially connected and perform inverse operations of the transmitter in the receiver. Then, the sparse channel is estimated at the receiver by using the semi blind sparse algorithm, where the parameters are optimized by using the Hybridized Drosophila with Virus Colony Search Optimization (HD‐VCSO). The objective function of the developed sparse channel estimation is the reduction of Minimum Mean Square Error (MMSE) in the channel. The performance of the developed sparse channel estimation algorithm is analyzed through different conventional algorithms to validate the effectiveness of the proposed algorithm.