Phase Transitions of Binary Lipid Mixtures: A Combined Study by Adiabatic Scanning Calorimetry and Quartz Crystal Microbalance with Dissipation Monitoring

Losada-Pérez, Patricia; Mertens, Nathalie; Medio-Vasconcelos, B. de; Slenders, Eli; Peeters, Marloes; Grinsven, Bart van; Gruber, Jonas; Glorieux, Christ; Pfeiffer, Helge; Wagner, Patrick; Thoen, Jan

doi:10.1155/2015/479318

Cited by 35 publications

(29 citation statements)

References 48 publications

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“…At relatively lower T (or higher T m ) s o is evidenced to exist as a macroscopic phase separated state via fluorescence experiments, AFM, and NMR. s o can disappear at physiological temperatures due to presence of Chol (11,(48)(49)(50)(51) or unsaturated lipids, (52)(53)(54)(55) which lower the T m of saturated lipids. At high (≳40 mol%) Chol concentrations macroscopic phase separations disappear.…”

Section: Tous(2)mentioning

confidence: 99%

“…The impact of Chol concentration on the structure and dynamics associated with liquid phase behavior in lipid bilayers was investigated using a CG ternary lipid mixture observed to achieve macroscopic phase separation in molecular dynamics simulation. Simulations of DPPC, DIPC, and Chol lipids at 0, 3,7,13,22,30,42,53, and 61 mol% Chol, maintaining DPPC and DIPC at equimolar ratios, were performed. Three 11 µs replicate trajectories of each system were sampled representing a total of 3 x 9 x 11 µs = 297 µs of simulation.…”

Section: A Spatial and Structural Equilibrationmentioning

confidence: 99%

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Regimes of Complex Lipid Bilayer Phases Induced by Cholesterol Concentration in MD Simulation

Pantelopulos

Straub

2018

Preprint

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Cholesterol is essential to the formation of phase separated lipid domains in membranes. Lipid domains can exist in different thermodynamic phases depending on the molecular composition, and play significant roles in determining structure and function of membrane proteins. We investigate the role of cholesterol in the structure and dynamics of ternary lipid mixtures displaying phase separation using Molecular Dynamics simulations, employing a physiologically-relevant span of cholesterol concentration. We find that cholesterol can induce formation of three regimes of phase behavior, I) miscible liquid disordered bulk, II) phase separated, domain registered coexistence of liquid disordered and liquid ordered and domains, and III) phase separated, domain-antiregistered coexistence of liquid-disordered and newly-identified nanoscopic gel domains composed of cholesterol threads we name "cholesterolic gel" domains. These findings are validated and discussed in the context of current experimental knowledge, models of cholesterol spatial distributions, and models of ternary lipid mixture phase separation.These collected observations substantially enhance our understanding of the role of Chol in complex phase behavior in ternary lipid mixtures and provide a framework for exploring structure and dynamics of domain formation in future computational, theoretical, and experimental investigations. ASSOCIATED CONTENT Supporting InformationSee supporting information for illustrative demonstrations of order parameters mix , ) , and f , explanation and illustraton of 50% miscibility mapping to mix , and visualizations of f and | f | in all systems. Additionally, order parameters measured for transleaflet clusters and illustrative demonstration of the clustering approach used is supplied.

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Section: Tous(2)mentioning

confidence: 99%

Section: A Spatial and Structural Equilibrationmentioning

confidence: 99%

Regimes of Complex Lipid Bilayer Phases Induced by Cholesterol Concentration in MD Simulation

Pantelopulos

Straub

2018

Preprint

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“…Although not specified on the current data sheet [33], older versions mention that this material is a mixture of alkanes. It is described as a solidliquid PCM with a melting region of [38][39][40][41][42][43] C, and it stores 174 kJ kg À1 between 35 and 50°C. Three samples were prepared.…”

Section: Experimental Details Rt42 Samplesmentioning

confidence: 99%

“…novel Peltier-element-based adiabatic scanning calorimeter (pASC), which provides greater user-friendliness and also allows smaller samples [31,32,37,38]. At the core of the pASC, as can be seen in the schematic depiction in Fig.…”

Section: Pascmentioning

confidence: 99%

A new calorimetric technique for phase change materials and its application to alkane-based PCMs

Duponchel

Longuemart

Glorieux

et al. 2016

Mater Renew Sustain Energy

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The correct determination of the phase transition behaviour of phase change materials (PCMs) is paramount for assessing their application potential. In this work, the merits of a novel calorimetric technique, Peltierelement-based adiabatic scanning calorimetry (pASC), for PCM characterisation are investigated, especially in comparison with the commonly-used differential scanning calorimeter (DSC). A comparative study of two alkanebased PCMs, the commercial mixture RT42 and the pure alkane tricosane (C23) with these two techniques shows that pASC provides data at much higher resolution than DSC, due to its operation in thermodynamic equilibrium. Specifically the rate-dependence and deformation that is inherent to DSC experiments is absent in pASC. In addition, the enthalpy of the PCM is directly obtained. pASC results easily conform to the accuracy limits that are proposed in literature for the transition temperature and storage capacity of PCMs.

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“…Since the lipid phase transition influences the surface potential of the liposome reflecting a sharp change in the ZP during the transition, it was proposed as a screening method for transition temperatures in complex systems, given its high sensitivity and small amount of sample required, that is, 70% less than that required in the use of conventional calorimeters. Many experimental studies dependent on temperature have been reported for lipid systems but predicting mathematical model for the phase behavior was absent [11][12][13].…”

Section: Introductionmentioning

confidence: 99%

Lipidic nanoparticules: a model function to predict the transition temperature of DPPC-DMPC mixtures

Buffo¹,

Sierra²,

Pedroni³

et al. 2017

Adv Mater Sci

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Thermotropic behavior of unilamellar liposomes prepared from binary mixtures of phosphatidylcholines in aqueous suspensions was analyzed by means of the temperature dependence of the Zeta Potential (ZP). This technique presents high sensitivity and small amount of sample required, that is, 70% less than that required in the use of conventional calorimeters. A model function is proposed to adjust the thermotropic behavior of the DPPC-DMPC system. From the adjustment, temperature transition (T transition ) data as a function of the composition are obtained. The objective of this paper is to propose, from those few T transition -composition data, a function that allows obtaining T transition for any composition of the mentioned system. Moreover, graph of phase boundaries for these mixed-lipid vesicles was constructed by plotting the delimiting temperatures of the main phase transition as a function of the lipid composition of the vesicle. The combination of the zeta potential technique and the mathematical processing of their data would be a simple and useful tool for determining the transition temperatures of more complex mixtures.

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Phase Transitions of Binary Lipid Mixtures: A Combined Study by Adiabatic Scanning Calorimetry and Quartz Crystal Microbalance with Dissipation Monitoring

Cited by 35 publications

References 48 publications

Regimes of Complex Lipid Bilayer Phases Induced by Cholesterol Concentration in MD Simulation

Regimes of Complex Lipid Bilayer Phases Induced by Cholesterol Concentration in MD Simulation

A new calorimetric technique for phase change materials and its application to alkane-based PCMs

Lipidic nanoparticules: a model function to predict the transition temperature of DPPC-DMPC mixtures

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