Phenolic acids and a static magnetic field change the expression of transforming growth factor β isoforms in amelanotic melanoma cells

Abstract

Background Melanoma is an aggressive type of cancer that can metastasize to numerous other organs. TGFβ is one of the key signaling pathways in melanoma progression. Previous studies on various types of cancer have shown that both: polyphenols and a static magnetic field (SMF) can be potential chemopreventive/therapeutic agents. Therefore, the aim of the study was to evaluate the effect of a SMF and selected polyphenols on the transcriptional activity of TGFβ genes in melanoma cells. Methods and results Experiments were performed on the C32 cell line treated with caffeic or chlorogenic acids, and with simultaneous exposure to a moderate-strength SMF. The RT-qPCR method was used to determine the mRNA level of genes encoding the TGFβ isoforms and their receptors. The concentration of the TGFβ1 and TGFβ2 proteins were also measured in the cell culture supernates. The first response of C32 melanoma cells to both factors is the reduction of TGFβ levels. Then, mRNA level of these molecules returned to values close to pre-treatment level by the end of experiment. Conclusion Our study results demonstrate the potential of polyphenols and a moderate-strength SMF to support cancer therapy by altering TGFβ expression, which is a very promising topic for the diagnosis and treatment of melanoma.

The Effect of a Rotating Magnetic Field on the Regenerative Potential of Platelets

“…Kimsa-Dudek et al [56] studied the effect of phenolic acids and a static magnetic field on the expression of TGF-β isoforms in amelanotic melanoma cells. They observed a significant decrease in the concentration of TGF-β1 and TGF-β2 proteins in cells treated with SMF and phenolic acid.…”

Spice-Derived Phenolic Compounds: Potential for Skin Cancer Prevention and Therapy

“…Caffeic acid also significantly upregulated mRNA expression of the Casp3 and caspase-3 activity in C32 cells [216]. Later, they reported that both phenolic acids in the same melanoma cell line in combination with SMF decreased TGFβ protein levels [217]. Moreover, ChA in C32 cells suppressed the viability of melanoma cells and the expression of genes of antioxidant enzymes superoxide dismutase (SOD1, SOD2) and glutathione peroxidase (GPX1), as well as activity of SOD, GPx, and catalase (CAT).…”