Phenolic Analysis and In Vitro Biological Activity of Red Wine, Pomace and Grape Seeds Oil Derived from Vitis vinifera L. cv. Montepulciano d’Abruzzo

Abstract

Grape pomace is commonly considered a waste product of monovarietal red wine production. Methods: HPLC-DAD analysis was performed to determine the polyphenol and flavonoid contents of all the extracts obtained from Montepulciano d’Abruzzo red wine and grape skins whereas, GC-MS was applied to the determination of fatty acid composition in grape seeds oil. Biological characterization involves antioxidant and antimicrobial assays for all the extracts and seeds oil; Their ability to inhibit α-glucosidase, α-amylase, α-tyrosinase, and ChE enzymes was also detected, together with anti-inflammatory activity on wine, grape skin extracts, and seeds oil by lipoxygenase (5-LOX) and LPS-stimulated macrophage release assays. Data indicate significative polyphenols content (199.31 ± 7.21 mgGAE/g), antioxidant (CUPRAC assay (1036.98 mgTE/g)), enzymatic inhibition (α-tyrosinase: 151.30 ± 1.20 mgKAE/g) and anti-inflammatory activities for wine-organic extract 2, while the antimicrobial activity of grape skin decoction is higher than those reported by wine extracts on three bacterial strains. Interestingly only dealcoholized wine and wine-aqueous extract exerts inhibitory effects on α-glucosidase (20.62 ± 0.23 mmolACAE/g and 19.81 ± 0.03 mmolACAE/g, respectively), while seeds oil is rich in oleic and linoleic acids. These results confirm the strong antioxidant properties of Montepulciano d’Abruzzo grape pomace, suggesting the potential use of this waste product as functional food supplements in the human diet and in cosmeceutics.

Phytochemical Studies on Two Unexplored Endemic Medicinal Plants of India, Barleria terminalis and Calacanthus grandiflorus

“…HPLC analysis confirmed the presence of potent phenolics and phenylethanoid glycoside (verbascoside). Bioactive compounds found in plants also play important role in pharmaceutics and food industry, where some secondary metabolites showed potential biological activities ( Nescatelli et al, 2017 ; Stefanucci et al, 2018 ; Uysal et al, 2019 ; Lekhak et al, 2021 ; Mollica et al, 2021 ; Patel and Ghane, 2021 ). Ranade et al (2016) concluded that among phenolics, GA and CA were found the most common and abundant in leaves and stem of B. prionitis .…”

Myeloperoxidase-Oxidized LDL Activates Human Aortic Endothelial Cells through the LOX-1 Scavenger Receptor

“…The latter drugs may include naturally occurring antioxidants with anti-inflammatory functions that have been demonstrated to inhibit LOX-1 expression and activity in vascular cells [ 37 ]. Food that is rich in polyphenols and flavonoids was shown to have strong antioxidant and anti-inflammatory activity [ 38 , 39 ]. Indeed, procyanidins were reported to significantly decrease oxidized LDL uptake in LOX-1 expressing cells in vitro by inhibiting the binding of the scavenger receptor to its modified LDL ligand.…”

Chemometric Optimization of Biologically Active Compounds Extraction from Grape Marc: Composition and Antimicrobial Activity

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