Phenolic characterization and antimicrobial activity of folk medicinal plant extracts for their applications in olive production

Abstract

Phytophthora spp is important in plant pathology due to the importance of the diseases it causes. In olive trees, severe damages are caused by the disease known as "dry branch" occasioned by Phytophthora nicotianae, P. citrophthora and P. palmivora. Much effort has been made to find efficient methods of control, with a low negative impact on environment. In this regard, treatment with plant extracts is a valid strategy. The aims of the present study are (i) to determine the polyphenol composition of extracts of Thymus vulgaris, Origanum vulgare, Matricaria recutita, and Larrea divaricata by CZE, (ii) correlate the analytical composition of these extracts with the inhibition on the mycelial growth, and (iii) determine the individual antimicrobial activity of the most active ingredients. A simple methodology was developed for the determination of catechin, naringenin, cinnamic acid, syringic acid, chlorogenic acid, apigenin, vanillic acid, luteolin, quercetin, and caffeic acid in plant extracts by CZE. The extraction of phenolic compounds in extract was performed by a miniaturized solid phase extraction using a home-made minicolumn packed with suitable filtering material (C18 , 50 mg). The optimized analyses conditions were: 30 mM boric acid buffer, pH 9.50; capillary, 57 cm full length, 50 cm effective length, 75 μm id, hydrodynamic injection 30 mbar, 2 s; 25 kV; 25°C, detection by UV absorbance at 290 nm. Sample results suggest that phenolic composition seems to have a great influence on inhibition of pathogens. The highest inhibitions of mycelial growth were observed for cinnamic acid and naringenin.

Recent advances in the application of capillary electromigration methods for food analysis and Foodomics

“…Polyphenol composition of four different plant extracts from Thymus vulgaris , Origanum vulgare , Matricaria recutita , and Larrea divaricate was studied in order to correlate their composition with the inhibition of the disease and to determine the antimicrobial capacity of each individual phenolic compound against Phytophthora spp . . Thus, a SPE followed by a CZE method using 30 mM boric acid buffer at pH 9.50 was applied to all extracts.…”

Determination of Total Apigenin in Herbs by Micellar Electrokinetic Chromatography with UV Detection

“…Şanli and Lunte determined flavonoids including API in Chamomile and Linden extracts with the use of capillary zone electrophoresis [ 12 ]. Boiteux et al studied antimicrobial activity of plant extracts of chamomile, oregano, thyme, and creosote bush [ 13 ]. Honegr et al used large-volume sample stacking with polarity switching for determination of natural polyphenols in Orthosiphon stamineus Benth [ 14 ].…”

In vitro and in vivo efficacy of Larrea divaricata extract for the management of Phytophthora palmivora in olive trees

“…In view of the above, L. divaricata extract represents a great alternative for the in vitro control of P. palmivora. Noteworthy is that the antimicrobial activity of L. divaricata extract has been related with the presence of phenolic compounds (3). In this study, piperine was the only alkaloid found in the extract by HPLC-MWD analysis.…”