Phenolic Content and Antioxidant Activity of Different Extracts From Ocimum Basilicum, Apium Graveolens and Lepidium Sativum Seeds

Abstract

The antioxidative properties and total phenolic contents of methanol, ethanol and water extracts from Ocimum basilicum, Apium graveolens and Lepidium sativum seeds were investigated and the results were compared with standards.The methanol extracts of the seeds had significantly higher (P < 0.05) superoxide radical scavenging activity with the concentration for 50% inhibition (IC 50 ) value of 98.73 mg/mL for O. basilicum, 121.57 mg/mL for A. graveolens and 166.16 mg/mL for L. sativum. The results indicated that all the seed extracts showed excellent H 2 O 2 scavenging activities. IC 50 values for H 2 O 2 scavenging activity by methanol extract of O. basilicum, A. graveolens, L. sativum and butylated hydroxyanisole were found to be as 49.9, 52.3, 65.2 and 49.6 mg/mL, respectively. O. basilicum methanol extract had significantly (P < 0.05) higher 1,1-diphenyl-2-picryl-hydrazyl scavenging effect than the other two seeds. The methanol extracts of three seeds were better in Fe +2 chelating activity, reducing power and higher in the content of total phenol as compared with ethanol and water extracts. PRACTICAL APPLICATIONSRecent epidemiological studies have strongly suggested that consumption of certain plant materials such as seeds, leaves, fruits and roots may reduce the risk of chronic diseases related to oxidative stress on account of their antioxidant activity and promote general health benefits. In this study, all seeds extracts exhibited good antioxidant activity (78.3-96.9%) at concentration 1 Corresponding 62 500 mg/mL. Total phenolic content in the seed extracts ranged from 51 to 92 mg GAE/100 g extract. In addition, there was a statistically significant correlation between the amount of phenolic compounds and antioxidant activity (R 2 = 0.9058, P < 0.05) in all the seed extracts. Therefore, O. basilicum, A. greveolens and L. sativum seed extracts have the potential to be developed into dietary supplements and nutraceuticals. 63 PHENOLIC CONTENT AND ANTIOXIDANT ACTIVITY 65 PHENOLIC CONTENT AND ANTIOXIDANT ACTIVITY

Antimicrobial, Antioxidant and Anti-inflammatory Characteristics of Combination (<i>Cassia fistula</i> and <i>Ocimum basilicum</i>) Extract as Natural Preservative to Control &amp; Prevent Food Contamination

“…Obtained cana fistula IC 50 results are in agreement with [40] while lower values were reported by [41]. Basil obtained IC 50 results was better than previously reported by [42] who reported higher values. Mixing the two plant aqueous extracts affected positively by arising inhibition % and lowering mix IC 50 into (29.88 µg/mL).…”

Apium GraveolensExtract Attenuates Adjuvant Induced Arthritis by Reducing Oxidative Stress

“…The major bioactive compounds detected in A. graveolens can include vitamins, minerals and a high amount of dietary fibers in addition to non‐nutrient chemicals such as a class of phenolic compounds (furanocoumarins, caffeic acid, ferulic acid, etc.) (Aydemir and Becerik ; Jung et al . ; Yao and Ren ; Baananou et al .…”

Authentication of herbal drug Tukhm‐e‐balango (Lallemantia royleana Benth.) using microscopic, pharmacognostic, and phytochemical characterization

“…Whereas, flavonoid contents were higher in O. basilicum (18.16 mg QE/g, DW) as compared to L. royleana (16.03 mg QE/g, DW) (Figure ). According to Aydemir and Becerik (), total phenolic contents in O. basilicum ranged from 74 to 92 mg GAE/100 g in different solvent extracts. Variations in phenolic contents of different solvent extracts are attributed to polarities of different compounds present in seeds.…”

“…Amanzadeh, Dehaghi, Gohari, Monsef‐Esfahani, and Ebrahimi () analyzed the antioxidant activity of essential oil of L. iberica by DPPH radical scavenging assay and reported that essential oil of aerial parts showed the highest radical scavenging potential. Aydemir and Becerik () evaluated the antioxidant activity of different extracts from O. basilicum, Lepidium sativum, and Apium graveolens seeds and recorded 56.4% scavenging activity in methanolic extract of O. basilicum seeds at a concentration 250 μg/ml. In literature, antioxidant activity of O. basilicum leaves (60.75%) has also been reported by Zakaria, Aziz, Lachimanan, Sreenivasan, and Rathinam ().…”