Phenotype and function of IgE-binding monocytes in equine Culicoides hypersensitivity

Larson, Elisabeth M.; Babasyan, Susanna; Wagner, Bettina

doi:10.1371/journal.pone.0233537

Cited by 17 publications

(26 citation statements)

References 69 publications

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“…There are only a few studies concerning equine monocytes because of the low availability of species-specific monoclonal antibodies [ 49 , 50 , 51 ]. Furthermore, there is a lack of studies connected with the influence of exercise on monocyte function in equids.…”

Section: Discussionmentioning

confidence: 99%

The Effect of Physical Training on Peripheral Blood Mononuclear Cell Ex Vivo Proliferation, Differentiation, Activity, and Reactive Oxygen Species Production in Racehorses

Witkowska-Piłaszewicz

Pingwara

Winnicka

2020

Antioxidants

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Physical activity has an influence on a variety of processes in an athlete’s organism including the immune system. Unfortunately, there is a lack of studies regarding racehorse immune cells, especially when the horse model is compared to human exercise physiology. The aim of the study was to determine changes in immune cell proliferation, lymphocyte populations, and monocyte functionality in trained and untrained racehorses after exercise. In this study, field data were collected. The cells from 28 racehorses (14 untrained and 14 well-trained) were collected before and after exercise (800 m at a speed of about 800 m/min) and cultured for 4 days. The expression of CD4, CD8, FoxP3, CD14, MHCII, and CD5 in PBMC, and reactive oxygen species (ROS) production, as well as cell proliferation, were evaluated by flow cytometry. In addition, IL-1β, IL-4, IL-6, IL-10, IL-17, INF-γ, and TNF-α concentrations were evaluated by ELISA. The creation of an anti-inflammatory environment in well-trained horses was confirmed. In contrast, a pro-inflammatory reaction occurred in untrained horses after training. In conclusion, an anti-inflammatory state occurs in well-trained racehorses, which is an adaptational reaction to an increased workload during training.

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Section: Discussionmentioning

confidence: 99%

The Effect of Physical Training on Peripheral Blood Mononuclear Cell Ex Vivo Proliferation, Differentiation, Activity, and Reactive Oxygen Species Production in Racehorses

Witkowska-Piłaszewicz

Pingwara

Winnicka

2020

Antioxidants

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“…In contrast, we observed the most consistent expression of CD90 in MSC cultured with 10% ePL media and expression of CD45 was almost undetectable in all media. Furthermore, we made an interesting observation of CD14 detection on the majority of MSC when using an equine-specific antibody for CD14 (Kabithe et al, 2010 ; Wagner et al, 2013 ; Bonelli et al, 2017 ; Schnabel et al, 2019 ; Larson et al, 2020 ; Patel et al, 2020 ). According to the ISCT, CD14 should not be expressed on human MSC (Dominici et al, 2006 ) as it is a glycolipid anchored membrane glycoprotein expressed on monocytes and macrophages (Tesfaigzi, 2006 ; Braun et al, 2010 ) and a leukocyte-secreted molecule in response to LPS stimulation and inflammation, as demonstrated in horses (Wagner et al, 2013 ).…”

Section: Discussionmentioning

confidence: 99%

Scalable Production of Equine Platelet Lysate for Multipotent Mesenchymal Stromal Cell Culture

Hagen

Lehmann

Aurich

et al. 2021

Front. Bioeng. Biotechnol.

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Translation of multipotent mesenchymal stromal cell (MSC)-based therapies is advancing in human and veterinary medicine. One critical issue is the in vitro culture of MSC before clinical use. Using fetal bovine serum (FBS) as supplement to the basal medium is still the gold standard for cultivation of many cell types including equine MSC. Alternatives are being explored, with substantial success using platelet lysate-supplemented media for human MSC. However, progress lags behind in the veterinary field. The aim of this study was to establish a scalable protocol for equine platelet lysate (ePL) production and to test the ePL in equine MSC culture. Whole blood was harvested into blood collection bags from 20 healthy horses. After checking sample materials for pathogen contamination, samples from 19 animals were included. Platelet concentrates were prepared using a buffy coat method. Platelets, platelet-derived growth factor BB, and transforming growth factor β1 concentrations were increased in the concentrates compared with whole blood or serum (p < 0.05), while white blood cells were reduced (p < 0.05). The concentrates were lysed using freeze/thaw cycles, which eliminated the cells while growth factor concentrations were maintained. Donor age negatively correlated with platelet and growth factor concentrations after processing (p < 0.05). Finally, all lysates were pooled and the ePL was evaluated as culture medium supplement in comparison with FBS, using adipose-derived MSC from four unrelated donor horses. MSC proliferated well in 10% FBS as well as in 10% ePL. However, using 5 or 2.5% ePL entailed highly inconsistent proliferation or loss of proliferation, with significant differences in generation times and confluencies (p < 0.05). MSC expressed the surface antigens CD90, CD44, and CD29, but CD73 and CD105 detection was low in all culture media. Adipogenic and osteogenic differentiation led to similar results in MSC from different culture media. The buffy coat method is useful to produce equine platelet concentrate with increased platelet and reduced white blood cell content in large scales. The ePL obtained supports MSC expansion similar as FBS when used at the same concentration (10%). Further investigations into equine MSC functionality in culture with ePL should follow.

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“…This disproportionately high frequency of IgE-binding monocytes suggested that IgE-binding monocytes are substantially responsible for IL-8 production, independent of IgER signaling. We further explored the mechanism of IL-8 production in IgE-binding monocytes after IgE cross-linking, which are also known to exhibit unique IgEmediated functional responses (51).…”

Section: Discussionmentioning

confidence: 99%

“…6A, 6B) (48), and IgE-binding monocytes, which are characterized by IgE 1 /MHCII high / CD14 low (Fig. 6A, 6B) (51). The majority of IgE 1 /MHCII high cells are IgE-binding monocytes.…”

Section: Il-8 Production By Ige-binding Monocytes and Basophils After Ige Cross-linkingmentioning

confidence: 94%

IgE-Binding Monocytes Have an Enhanced Ability to Produce IL-8 (CXCL8) in Animals with Naturally Occurring Allergy

Larson

Babasyan

Wagner

2021

The Journal of Immunology

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IL-8 is a potent chemokine that recruits neutrophils and basophils to promote inflammation in many species. IL-8 is produced by many cell types, including monocytes. In this study, we report a novel role for IgE-binding monocytes, a rare peripheral immune cell type, to promote allergic inflammation through IL-8 production in a horse model of natural IgE-mediated allergy. We developed a mAb with confirmed specificity for both recombinant and native equine IL-8 for flow cytometric analysis. Equine IL-8 was produced by CD14+/MHC class II+/CD16− monocytes, including a subpopulation of IgE-binding monocytes, following stimulation with LPS. In addition, IgE cross-linking induced IL-8 production by both peripheral blood basophils and IgE-binding monocytes. IL-8 production was compared between healthy horses and those with a naturally occurring IgE-mediated skin allergy, Culicoides hypersensitivity. Allergic horses had significantly higher percentages of IL-8+ IgE-binding monocytes after IgE cross-linking. In contrast, frequencies of IL-8+ basophils after IgE cross-linking were similar in all horses, regardless of allergic disease, highlighting IgE-binding monocytes as a novel source of IL-8 during allergy. We concluded that IgE-binding monocytes from allergic individuals have an increased capacity for IL-8 production and likely contribute to the recruitment of innate immune cells during IgE-mediated allergy and promotion of inflammation during repeated allergen contact.

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Phenotype and function of IgE-binding monocytes in equine Culicoides hypersensitivity

Cited by 17 publications

References 69 publications

The Effect of Physical Training on Peripheral Blood Mononuclear Cell Ex Vivo Proliferation, Differentiation, Activity, and Reactive Oxygen Species Production in Racehorses

The Effect of Physical Training on Peripheral Blood Mononuclear Cell Ex Vivo Proliferation, Differentiation, Activity, and Reactive Oxygen Species Production in Racehorses

Scalable Production of Equine Platelet Lysate for Multipotent Mesenchymal Stromal Cell Culture

IgE-Binding Monocytes Have an Enhanced Ability to Produce IL-8 (CXCL8) in Animals with Naturally Occurring Allergy

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