Phenotypes of stem cells from diverse origin

Abstract

Stem cells have turned into promising tools for studying the mechanisms of development, regeneration, and for cell therapy of various disorders. Stem cells are found in the embryo and in most adult tissues participating in endogenous tissue regeneration.

Adult Stem Cell Mobilization Enhances Intramembranous Bone Regeneration: A Pilot Study

“…2C). The output variables included [19,55] Lineage panel* APC HSC(À) [48], EPC(À), MSC(À) * Recognizes CD3e, CD11b, CD45R/B220, Ly-76, Ly-6G, and Ly-6C; PE = phycoerythrin; FITC = fluorescein isothiocyanate; APC = allophycocyanin; PerCP = peridinin-chlorophyll-protein complex; MSC = mesenchymal stem cell; HSC = hematopoietic stem cell; EPC = endothelial progenitor cell.…”

CD133 positive progenitor endothelial cell lines from human cord blood

“…Additionally, in each individual EPC donor, different stages of their differentiation are supposed to be present and, furthermore, EPC phenotype may differ according to the nature of the tissue they originate from (12).…”

“…After the separation step, several markers (CD45, CD13, CD31, CD105, CD144, CD202b, Ulex Agglutinin-1 (UEA-1)) were usually used to characterize the obtained cell populations and to discriminate the putative EPCs from hematopoietic stem cells (HSCs) (12,13). Keeping in mind that both endothelial and hematopoietic lineages originate from a common progenitor-hemangioblast-the closer the progenitors are, the higher are their similarities.…”

Skin extracellular matrix components accelerate the regenerative potential of Lin− cells

“…Several stem and progenitor cell populations, expressing significant regenerative properties, are found in the bone marrow [13]. To obtain pure cell populations is still a challenge as the primitive cells do not possess a well defined phenotype; the same marker could be expressed on the surface of different stem/progenitor cells [14][15][16]. Bone marrow cells bearing no surface markers, specific for mature cells (Lin¯ cells) were used in our study.…”