Phenotypic and functional characterization in vitro of a multipotent epithelial cell present in the normal adult human breast

Stingl, John; Eaves, Connie J.; Kuusk, Urve; Emerman, Joanne T.

doi:10.1111/j.1432-0436.1998.00201.x

Cited by 216 publications

(65 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although no mention of receptor status was included in their description, on the basis of the figures, none of these lesions demonstrated the characteristic apocrine morphology. The authors concluded that CD10 positivity in metastatic tumors cannot rule out the breast as primary, and related the phenotype to the CD10-positive progenitor cells capable of differentiating towards luminal epithelial cells and MECs described by Stingl et al [34]. …”

Section: Discussionmentioning

confidence: 99%

CD10 Immunohistochemical Expression in Apocrine Lesions of the Breast

Kővári

Báthori

2015

Pathobiology

View full text Add to dashboard Cite

Objective: In the breast, CD10 is expressed by myoepithelial cells (MECs), and apocrine metaplasia has also been mentioned as being positive with this marker. Apocrine lesions have been explored for the expression of CD10. Methods: The apocrine lesions studied included 11 cysts, 6 cases of apocrine adenosis, 2 of apocrine metaplasia or hyperplasia in papilloma, 13 ductal carcinomas in situ (DCIS) and invasive carcinomas (14 ductal and 4 lobular). Results: Benign apocrine lesions showed complete or partial luminal CD10 staining, although most cases included parts without staining, and 2 lesions were completely negative. The MECs were often but not always positive. Nine of the 13 cases of apocrine DCIS displayed no luminal staining, but 4 demonstrated very focal luminal positivity. The MECs around the DCIS showed a spectrum of staining from nil to strong and complete. Only 4 invasive carcinomas demonstrated luminal/membranous staining. Cytoplasmic CD10 positivity was seen focally in 4 invasive cancers and in 3 DCIS. Conclusion: CD10 positivity is luminal/membranous in most benign apocrine lesions, the staining being nonuniversal and sometimes focal. Analogous staining in apocrine malignancies seems rarer in DCIS and even rarer in invasive apocrine carcinomas, but atypical cytoplasmic positivity may also occur. CD10 is not an ideal myoepithelial marker in apocrine lesions.

show abstract

Section: Discussionmentioning

confidence: 99%

CD10 Immunohistochemical Expression in Apocrine Lesions of the Breast

Kővári

Báthori

2015

Pathobiology

View full text Add to dashboard Cite

show abstract

“…A third distinct cell type has also been described. Based on its surface marker profile (MUC − /ESA + ), presumably it is located in between the luminal epithelial (MUC + /ESA + ) and myoepithelial (MUC − /ESA − ) layer [Stingl et al, 1998;Gudjonsson, et al, 2002b]. Evidence for such a location has been provided also from studies of the mouse mammary gland, where they are referred to as small light cells, are abluminal in location, and rest either directly on the basement membrane or on the luminally oriented surface of a myoepithelial cell [Smith and Chepko, 2001].…”

Section: Evidence That Mammary Stem Cells Existmentioning

confidence: 99%

“…With flow cytometry, sialomucin-positive, CD10-negative, epithelial-specific antigen-positive (Muc + /CD10 − /ESA + ), Muc − /CD10 + /ESA + , and Muc − /CD10 − /ESA − cellular subpopulations were isolated and their functional potential was analyzed in culture assays [Stingl et al, 1998[Stingl et al, , 2001. Two-dimensional cultures of Muc − /CD10 + /ESA + primary cells, with calf serum-containing media, yielded mixed colonies with ESA + /Muc + luminal cells at the center surrounded by K14 + teardrop-shaped myoepithelial-like cells at the periphery.…”

Section: Reagents and Materialsmentioning

confidence: 99%

“…Conversely, Muc + /CD10 − /ESA + cells gave rise to alveolar-like, K18 + luminal cells, and Muc − /CD10 + /ESA − cells gave rise to myoepithelial cells in 2D. When cultured in 3D collagen gels, Muc + /CD10 − /ESA + cells gave rise to K18 + /19 + spheroids, whereas Muc − /CD10 − /ESA + cells generated spheroids with a K14 + /α-SMA − outer layer and a Muc + interior layer when cultured in the absence of EGF [Stingl et al, 1998]. In the presence of EGF, the Muc − /CD10 − /ESA + cells generated branching terminal ductal lobular unit (TDLU)-like structures [Stingl et al, 2001].…”

Section: Reagents and Materialsmentioning

confidence: 99%

See 1 more Smart Citation

Culturing Mammary Stem Cells

LaBarge

Petersen

Bissell

2007

Culture of Human Stem Cells

View full text Add to dashboard Cite

“…Recently, we and others have provided evidence that the stem cells of the mouse and human mammary gland may be contained within the luminal epithelial lineage [5,[97][98][99][100] (reviewed in [101]). Thus, we have shown previously in primary cultures that a subset of the luminal epithelial cells convert to myoepithelial cells in culture, suggesting the existence of a progenitor cell [99].…”

Section: E6-and E7-immortalized Cell Lines As a Model For Breast Morpmentioning

confidence: 99%

Immortalization protocols used in cell culture models of human breast morphogenesis

Gudjonsson

Villadsen²,

Rønnov‐Jessen³

et al. 2004

CMLS, Cell. Mol. Life Sci.

View full text Add to dashboard Cite

Defining the key players in normal breast differentiation is instrumental to understanding how morphogenesis becomes defective during breast cancer progression. During the past 2 decades much effort has been devoted to the development of technologies for purification and expansion of primary human breast cells in culture and optimizing a relevant microenvironment, which may help to define the niche that regulates breast differentiation and morphogenesis. In contrast to the general property of cancer, normal human cells have a finite lifespan. After a defined number of population doublings, normal cells enter an irreversible proliferation-arrested state referred to as replicative senescence. To overcome this obstacle for continuous long-term studies, replicative senescence can be bypassed by treatment of cells with chemical agents such as benzopyrene, by radiation or by transfection with viral oncogenes or the gene for human telomerase (human telomerase reverse transcriptase, hTERT). A drawback of some of these protocols is a concurrent introduction of chromosomal changes, which sometimes leads to a transformed phenotype and selection of a subpopulation, which may not be representative of the tissue of origin. In recent years, we have sought to establish immortalized primary breast cells, which retain crucial characteristics of their original in situ tissue pattern. This review discusses various approaches to immortalization of breast-derived epithelial and stromal cells and the application of such cell lines for studies on human breast morphogenesis.

show abstract

Phenotypic and functional characterization in vitro of a multipotent epithelial cell present in the normal adult human breast

Cited by 216 publications

References 39 publications

CD10 Immunohistochemical Expression in Apocrine Lesions of the Breast

CD10 Immunohistochemical Expression in Apocrine Lesions of the Breast

Culturing Mammary Stem Cells

Immortalization protocols used in cell culture models of human breast morphogenesis

Contact Info

Product

Resources

About