Phenotypic and Genotypic Antimicrobial Resistance of Lactococcus Sp. Strains Isolated from Rainbow Trout (Oncorhynchus Mykiss)

Abstract

A current profile of antimicrobial resistance and plasmid of 29 Lactococcus garvieae and one Lactococcus lactis strains isolated from rainbow trouts (Oncorhynchus mykiss) from farms throughout Turkey were investigated. All isolates were sensitive to penicillin G (90%), ampicillin (86.7%), florfenicol (83.3%), amoxicillin (80.1%), and tetracycline (73.4%), and resistant to trimethoprim+sulfamethoxazole (86.6%) and gentamycin (46.6%) by disc diffusion method. Twenty-eight (93%) isolates had two to seven antibiotic resistance genes (ARGs) determined by PCR. The most prevalent ARGs were tetracycline (tetB), erythromycin (ereB), and β-lactam (blaTEM). Bacterial strains were also screened for plasmid DNA by agarose gel electrophoresis and two strains harboured plasmids, with sizes ranging from 3 to 9 kb.

Efficacy of a formalin‐inactivated Lactococcus garvieae vaccine in farmed grey mullet (Mugil cephalus)

“…Antibiotics are commonly used to treat lactococcosis (Raissy & Ansari, 2011; Türe & Boran, 2015). However, most of the strains have gained resistance to several antibiotics, restricting their use and posing serious challenge during a lactococcosis outbreak in fish culture.…”

Identification of bacterial pathogens and determination of their antibacterial resistance profiles in some cultured fish in Turkey

“…In the last decade there has started to be awareness about the disease in Turkey. It was demonstrated that β-lactam antibiotics and florfenicol were the most effective compounds in growth inhibition of L. garvieae (25). In the present study, L. garvieae was isolated from fish from two trout farms.…”

“…Antibiotic discs were dispensed onto the surface of the plates, and the plates were incubated aerobically for 22 h at 30 and 37°C for Gram positive and negative bacteria respectively. After incubation, the zone diameters were measured (25).…”

Lactococcus garvieae İzolatlarının Antimikrobiyal Direnç Profillerinin Fenotipik ve Genotipik Olarak Belirlenmesi

“…Ülkemizde ve dünyada L. garvieae izolatlarının sahip oldukları antibiyotik direnç genlerinin ortaya konulduğu sınırlı sayıda çalışma bulunmaktadır. Türe ve Boran [26] [22]. Aynı çalışmada çalışmamızda bulduğumuz tek tetrasiklin direnç geni olan tetD ise %15,3 oranında saptanmıştır.…”

“…İran'da yapılan çalışmada 49 L. garvieae izolatının %65,3'ü enrofloksasin'e, %42,8'i eritromisin'e, %40,8'i kloramfenikol ve trimetoprim+sulfametoksazol ve %38,7'sinin tetrasiklin'e karşı dirençli olduğu bulunmuş ve test edilen 49 izolatın tamamını en az bir tetrasiklin direnç geni taşıdığı saptanmıştır [22]. Ülkemizde L. garvieae izolatlarında antibiyotik direnç genlerinin varlığının belirlendiği bir çalışmada izolatların en yaygın olarak tetrasiklin (tetB) direnç geni taşıdığı ortaya konulmuştur [26].…”