Phenotypic and genotypic approach to characterize a Trueperella pecoris strain isolated from necrotic vestibulitis of a camel (Camelus dromedarius)

Abstract

Significance and Impact of the Study:The current study provides a first detailed characterization of a strain of Trueperella pecoris isolated from necrotic vestibulitis of a 10-year-old camel. The T. pecoris strain was successfully identified by CAMP-like hemolytic reactions, API-Coryne test system and MALDI-TOF MS. Also, five molecular target: 16S rRNA gene, 16S-23S rDNA intergenic spacer region is not a gene, gap, tuf and rpoB of T. pecoris were evaluated by partial sequence analyses of the amplified target.

Identification of the novel potential pathogen Trueperella pecoris with interspecies significance by LAMP diagnostics

“…Trueperella pecoris is a novel bacterial species that appears to be related to several animal diseases, including mastitis in cattle, bronchopneumonia in pigs, and impairments of the reproductive tract in ruminants 1 , 4 . In addition, the presence of T. pecoris in the bovine mammary gland suggests that human infection through consumption of contaminated milk should also be considered, as the human pathogenic properties of Trueperella spp.…”

“…Strains were derived from the inhouse culture collection of the Institute of Food Quality and Food Safety, University of Veterinary Medicine Hannover, Foundation (Hannover, Germany), the German Collection of Microorganisms and Cell Cultures GmbH (Braunschweig, Germany), the National Collection of Type Cultures (Salisbury, UK), and from the RIPAC-LABOR GmbH (Potsdam, Germany, ENOVAT European strain database). For inclusivity testing, previously described T. pecoris strains originating from cattle and camel 1 , 4 as well as further T. pecoris isolates were considered (Table 1 ). Positive results were defined by the appearance of a fluorescence signal associated with a specific melting temperature.…”

“…However, other species of the genus Trueperella , such as T. abortisuis and T. pyogenes have also been associated with pathological manifestations in pigs, including abortion or multi-organ inflammation 2 , 3 . Recently, another T. pecoris strain was isolated from the necrotic vestibulitis of a camel 4 .…”