Phenotypic and genotypic characterization of antibiotic resistance of Salmonella Heidelberg in the south of Brazil

Soares, Luana Sielski Galvão; Casella, Tiago; Kawagoe, Eric Kazuo; Filho, Vilmar Benetti; Omori, Wellington Pine; Nogueira, Mara Corrêa Lelles; Wagner, Glauber; Oliveira, Rafael Rodrigues de; Stahlhofer, Sabine Rippel; Ferreira, Fabienne Antunes; Tondo, Eduardo César; Lindner, Juliano De Dea

doi:10.1016/j.ijfoodmicro.2023.110151

Cited by 4 publications

(1 citation statement)

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“…In North America, this serovar has been commonly isolated in broilers, layers, turkeys, and even pigs [31][32][33]. In Brazil, it has been frequently found in poultry farms since the year 2000 [8,9,[34][35][36][37].…”

Section: Discussionmentioning

confidence: 99%

Rapid DNA Detection of Salmonella enterica Typhimurium and Heidelberg from Poultry Samples

Mathias,

Souza,

Kipper

et al. 2024

Poultry

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The Salmonella enterica serovars Typhimurium (S. Typhimurium), Heidelberg (S. Heidelberg), and their monophasic variants (S. 1,4,[5],12:i:-, S. 1,4,[5],12:r:- and S. 1,4,[5],12:-:1,2) are highly disseminated in poultry farming and can contaminate chicken meat, eggs, and other foods of avian origin. A time-consuming bacteriological and serological analysis is usually required to identify serovars by traditional methods. Incomplete and inconclusive serological results are frequent in routine analysis, mainly due to the occurrence of bacterial isolates presenting similar antigenic profiles. Molecular biology assays have been developed to improve the detection of specific Salmonella serovars and strains. This study aimed to develop a multiplex real-time PCR (SHTAmp) for the rapid DNA detection of S. Typhimurium, S. Heidelberg, and their monophasic variants from poultry samples. The methodology was used in the analysis of 147 field isolates from Brazilian poultry flocks previously evaluated with serological analysis. The results demonstrated that it was able to specifically and rapidly detect 21 S. Typhimurium and 57 S. Heidelberg isolates with complete antigenic formulae. Furthermore, SHTAmp was able to differentiate nine S. Typhimurium and 44 S. Heidelberg isolates with incomplete serological formulae (monophasic and aphasic variants). The complete methodology was also successfully used to detect these bacteria directly from 34 poultry samples after pre-enrichment in buffered peptone water (BPW). In conclusion, SHTAmp is a fast and accurate method to detect the two frequent and concerning serovars S. Typhimurium and S. Heidelberg directly from poultry samples.

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