Phenotypic and Genotypic Characterization of Recently Isolated Multidrug-Resistant Acinetobacter baumannii Clinical and Aquatic Strains and Demonstration of Silver Nanoparticle Potency

Gheorghe-Barbu, Irina; Corbu, Viorica Maria; Vrancianu, Corneliu Ovidiu; Marinas, Ioana Cristina; Popa, Marcela; Dumbravă, Andreea Ștefania; Niță-Lazăr, Mihai; Pecete, Ionut; Muntean, Andrei Alexandru; Popa, Mircea Ioan; Marinescu, Liliana; Ficai, Denisa; Ficai, Anton; Czobor Barbu, Ilda

doi:10.3390/microorganisms11102439

Cited by 3 publications

(1 citation statement)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The reagents isopropyl alcohol, NH 3 solution 25%, hydrogen peroxide 35%, HCl 37%, MPTMS, and MPTES were purchased from Sigma-Aldrich (Darmstadt, Germany). The dithizone was acquired from Chinoin (Ciudad de Mexico, Mexico), the thioindigo was from (TCI-Tokyo Chemical Industry Co., Tokyo, Japan), and the AgNPs used were synthesized and characterized according to previous studies [17,18]. The AgNPs used in this study were synthesized by the solvothermal method with polyethylene glycol as a reduction component, at 260 • C and 3 bar pressure for 1 h. The results in the case of the solvothermal reaction showed that the nanoparticles were spherical with an average size of 50 nm (HT4 synthesis from our previous paper [17]).…”

Section: Chemicalsmentioning

confidence: 99%

Decoration of a Glass Surface with AgNPs Using Thio-Derivates for Environmental Applications

Ilie,

Spoială,

Motelica

et al. 2024

Coatings

Self Cite

View full text Add to dashboard Cite

The aim of this study is to decorate a glass surface with silver nanoparticles (AgNPs) and further prove its efficiency in the removal of some thio-derivatives—potential pollutants from water. Therefore, grafting the surface of glass-based platforms with AgNPs will strongly influence their interaction with other substances or molecules. The most commonly used molecules for glass-based platform functionalization/modification are organosilanes. In this case, the main interest is in thioalkyl organosilanes because, after silanization, the thio (-SH) functional groups that have a high affinity for AgNPs can intermediate their binding on the surface. By decorating the glass platforms with AgNPs, these surfaces become active for the adsorption of dyes from wastewater. Certainly, in this case, the dyes must bear -SH groups to ensure a high affinity for these surfaces. Therefore, the desired purpose of this study was to develop glass-based platforms decorated with AgNPs able to bind model molecules—dyes from aqueous media (dithizone—DIT and thioindigo—TIO), with these platforms being potentially used for environmental applications.

show abstract

Section: Chemicalsmentioning

confidence: 99%

Decoration of a Glass Surface with AgNPs Using Thio-Derivates for Environmental Applications

Ilie,

Spoială,

Motelica

et al. 2024

Coatings

Self Cite

View full text Add to dashboard Cite

show abstract

Phenotypic and Genotypic Characterization of Resistance and Virulence Markers in Candida spp. Isolated from Community-Acquired Infections in Bucharest, and the Impact of AgNPs on the Highly Resistant Isolates

Corbu,

Georgescu,

Marinas

et al. 2024

JoF

View full text Add to dashboard Cite

Background: This study aimed to determine, at the phenotypic and molecular levels, resistance and virulence markers in Candida spp. isolated from community-acquired infections in Bucharest outpatients during 2021, and to demonstrate the efficiency of alternative solutions against them based on silver nanoparticles (AgNPs). Methods: A total of 62 Candida spp. strains were isolated from dermatomycoses and identified using chromogenic culture media and MALDI-TOF MS, and then investigated for their antimicrobial resistance and virulence markers (VMs), as well as for metabolic enzymes using enzymatic tests for the expression of soluble virulence factors, their biofilm formation and adherence capacity on HeLa cells, and PCR assays for the detection of virulence markers and the antimicrobial activity of alternative solutions based on AgNPs. Results: Of the total of 62 strains, 45.16% were Candida parapsilosis; 29.03% Candida albicans; 9.67% Candida guilliermondii; 3.22% Candida lusitaniae, Candia pararugosa, and Candida tropicalis; and 1.66% Candida kefyr, Candida famata, Candida haemulonii, and Candida metapsilosis. Aesculin hydrolysis, caseinase, and amylase production were detected in the analyzed strains. The strains exhibited different indices of adherence to HeLa cells and were positive in decreasing frequency order for the LIP1, HWP1, and ALS1,3 genes (C. tropicalis/C. albicans). An inhibitory effect on microbial growth, adherence capacity, and on the production of virulence factors was obtained using AgNPs. Conclusions: The obtained results in C. albicans and Candida non-albicans circulating in Bucharest outpatients were characterized by moderate-to-high potential to produce VMs, necessitating epidemiological surveillance measures to minimize the chances of severe invasive infections.

show abstract

Tracing Acinetobacter baumannii’s Journey from Hospitals to Aquatic Ecosystems

Gheorghe-Barbu,

Dragomir,

Gradisteanu Pircalabioru

et al. 2024

Microorganisms

View full text Add to dashboard Cite

Background: This study provides a comprehensive analysis of Acinetobacter baumannii in aquatic environments and fish microbiota by integrating culture-dependent methods, 16S metagenomics, and antibiotic resistance profiling. Methods: A total of 83 A. baumannii isolates were recovered using culture-dependent methods from intra-hospital infections (IHI) and wastewater (WW) and surface water (SW) samples from two southern Romanian cities in August 2022. The antibiotic susceptibility was screened using disc diffusion, microdilution, PCR, and Whole Genome Sequencing assays. Results: The highest microbial load in the analyzed samples was found in Glina, Bucharest, for both WW and SW samples across all investigated phenotypes. For Bucharest isolates, the resistance levels corresponded to fluoroquinolones > aminoglycosides > β-lactam antibiotics. In contrast, A. baumannii from upstream SW samples in Târgoviște showed the highest resistance to aminoglycosides. The blaOXA-23 gene was frequently detected in IHI, WW, and SW isolates in Bucharest, but was absent in Târgoviște. Molecular phylogeny revealed the presence of ST10 in Târgoviște isolates and ST2 in Bucharest isolates, while other minor STs were not specifically correlated with a sampling point. Using 16S rRNA sequencing, significant differences in microbial populations between the two locations was identified. The low abundance of Alphaproteobacteria and Actinobacteria in both locations suggests environmental pressures or contamination events. Conclusions: These findings indicate significant fecal contamination and potential public health risks, emphasizing the need for improved water quality monitoring and management.

show abstract

Phenotypic and Genotypic Characterization of Recently Isolated Multidrug-Resistant Acinetobacter baumannii Clinical and Aquatic Strains and Demonstration of Silver Nanoparticle Potency

Cited by 3 publications

References 45 publications

Decoration of a Glass Surface with AgNPs Using Thio-Derivates for Environmental Applications

Decoration of a Glass Surface with AgNPs Using Thio-Derivates for Environmental Applications

Phenotypic and Genotypic Characterization of Resistance and Virulence Markers in Candida spp. Isolated from Community-Acquired Infections in Bucharest, and the Impact of AgNPs on the Highly Resistant Isolates

Tracing Acinetobacter baumannii’s Journey from Hospitals to Aquatic Ecosystems

Contact Info

Product

Resources

About