Phenotypic and genotypic characterization of levofloxacin- and moxifloxacin-resistant Mycobacterium tuberculosis clinical isolates in southern China

Hameed, H. M. Adnan; Tan, Yi; Islam, Mahmudul; Guo, Liangxing; Chhotaray, Chiranjibi; Wang, Shuai; Li, Yong; Gao, Yurui; Tan, Shouyong; Yew, Wing Wai; Zhong, Nanshan; Liu, Jianxiong; Zhang, Tianyu

doi:10.21037/jtd.2019.11.03

Cited by 21 publications

(17 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A study from mid-East of China showed that 37% of fluoroquinolone resistance events were in MDR M. tuberculosis isolates [ 10 ]. And a total of 76% LFX resistance and 73% MFX resistance MTB isolates were identified in a study from southern China [ 11 ]. Another report from Shanghai revealed that among FQ-resistant M. tuberculosis strains, 44% were multidrug-resistant isolates [ 12 ].…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization of multidrug-resistant tuberculosis against levofloxacin, moxifloxacin, bedaquiline, linezolid, clofazimine, and delamanid in southwest of China

Jiao

Xing

et al. 2021

BMC Infect Dis

View full text Add to dashboard Cite

Objectives To explore the drug susceptibility of levofloxacin (LFX), moxifloxacin (MFX), bedaquiline (BDQ), linezolid (LZD), clofazimine (CFZ) and delamanid (DLM) against multidrug resistant tuberculosis (MDR-TB) isolates from drug resistance survey of southwest China, and to illustrate the genetic characteristics of MDR-TB isolates with acquired drug resistance. Methods A total of 339 strains were collected from smear-positive TB patients in the drug resistance survey of southwest China between January 2014 and December 2016. The MICs for the above mentioned drugs were determined for MDR-TB by conventional drug susceptibility testing. Genes related to drug resistance were amplified with their corresponding pairs of primers. Results MDR was observed in 88 (26.0%; 88/339) isolates. LFX had the highest resistance rate (50.0%; 44/88), followed by MFX (38.6%; 34/88). The resistance rate to LZD, CFZ, and DLM was 4.5% (4/88), 3.4% (3/88), and 4.5% (4/88), respectively, and the lowest resistance rate was observed in BDQ (2.3%; 2/88). Of the 45 isolates resistant to LFX and MFX, the most prevalent resistance mutation was found in gyrA with the substitution of codon 94 (34/45, 75.6%). Two strains with CFZ - BDQ cross resistance had a mutation in the Rv0678 gene. Of the four LZD resistant isolates, two carried mutations in rplC gene. For the four isolates resistant to DLM, one isolate had mutations in codon 318 of fbiC gene, and two isolates were with mutations in codon 81 of ddn gene. Conclusion This study provided evidence of the usefulness of new anti-TB drugs in the treatment of MDR-TB in China.

show abstract

Section: Discussionmentioning

confidence: 99%

Molecular characterization of multidrug-resistant tuberculosis against levofloxacin, moxifloxacin, bedaquiline, linezolid, clofazimine, and delamanid in southwest of China

Jiao

Xing

et al. 2021

BMC Infect Dis

View full text Add to dashboard Cite

show abstract

“…A study from mid-East of China showed that 37% of uoroquinolone resistance events were in MDR M. tuberculosis isolates [10]. And a total of 76% LFX resistance and 73% MFX resistance MTB isolates were identi ed in a study from southern China [11]. Another report from Shanghai revealed that among FQ-resistant M. tuberculosis strains, 44% were multidrug-resistant isolates [12].…”

Section: Discussionmentioning

confidence: 99%

Molecular Characterization of Levofloxacin, Moxifloxacin, Bedaquiline, Linezolid, Clofazimine, and Delamanid against Multidrug-resistant Tuberculosis in Southwest of China

He¹,

Jiao²,

Xing³

et al. 2021

Preprint

View full text Add to dashboard Cite

Objectives: To explore the drug susceptibility of levofloxacin (LFX), moxifloxacin (MFX), bedaquiline (BDQ), linezolid (LZD), clofazimine (CFZ) and delamanid (DLM) against multidrug resistant tuberculosis (MDR-TB) isolates from drug resistance survey of southwest China, and to illustrate the genetic characteristics of MDR-TB isolates with acquired drug resistance. Methods: A total of 339 strains were collected from smear-positive TB patients in the drug resistance survey of southwest China between January 2014 and December 2016. The MICs were determined for MDR-TB identified by conventional drug susceptibility testing. Genes related to drug resistance were amplified with their corresponding pairs of primers. Results: MDR was observed in 88 (26.0%; 88/339) isolates. LFX had the highest resistance rate (50.0%; 44/88), followed by MFX (38.6%; 34/88). The resistance rate to LZD, CFZ, and DLM was 4.5% (4/88), 3.4% (3/88), and 4.5% (4/88), respectively, and the lowest resistance rate was observed in BDQ (2.3%; 2/88). Of the 45 isolates resistant to LFX and MFX, the most prevalent resistance mutation was found in gyrA with the substitution of codon 94 (34/45, 75.6%). All two CFZ - BDQ cross resistant strains had a mutation in the Rv0678 gene. Two of four LZD resistant isolates carried mutations in rplC gene. Of the four isolates resistant to DLM, one isolate with the mutations in codon 318 of fbiC gene and two isolates in codon 81 of ddn gene. Conclusion: This study provided evidence for further application of new anti-TB drugs in the treatment of MDR-TB in China.

show abstract

“…Pulmonary tuberculosis, also known as the white plague, is a chronic infectious disease caused by Mycobacterium tuberculosis and is mainly transmitted through the respiratory tract (1)(2)(3)(4). According to the World Health Organization, pulmonary tuberculosis affects tens of millions of people worldwide each year, and the death toll can reach 3 million annually, making pulmonary tuberculosis one of the infectious diseases with the highest incidence and mortality worldwide, with China being one of the countries with the highest incidence.…”

Section: Introductionmentioning

confidence: 99%

Effects of comprehensive nursing intervention combined with respiratory functional exercises on pulmonary function and self-care ability in patients with pulmonary tuberculosis: results of a randomized trial

Xu¹,

Chen²,

Li³

2021

Ann Palliat Med

View full text Add to dashboard Cite

Background: This study was carried out based on the background that pulmonary tuberculosis seriously threatens people's physical health while the current treatment has certain limitations.Methods: A total of 150 patients with pulmonary tuberculosis who were admitted to Wuhan Jinyintan Hospital from January 2017 to December 2018 were selected as study participants. Patients were randomly divided into a control group (n=75) who were treated with routine nursing, and an experimental group (n=75) who were treated with comprehensive nursing intervention combined with respiratory functional exercises.Following treatment, the pulmonary function, self-care ability, and other indexes of the patients in both groups were compared and analyzed.Results: After nursing intervention, the pulmonary function and self-care ability of patients in the experimental group were significantly higher than those in the control group. The scores of the 6-minute walking distance (6MWD) and modified Medical Research Council (mMRC) dyspnea scale of patients in both groups were significantly higher after nursing intervention compared to before intervention. Furthermore, after intervention, the scores in the experimental group were significantly higher than those in the control group. The partial pressure of oxygen (PaO 2 ), partial pressure of carbon dioxide (PaCO 2 ), and the quality of life of patients in the experimental group after intervention were significantly better than those in the control group.Conclusions: Comprehensive nursing intervention combined with respiratory functional exercises can significantly improve the pulmonary function, self-care ability, and quality of life of patients with pulmonary tuberculosis, with obvious clinical efficacy.

show abstract

Phenotypic and genotypic characterization of levofloxacin- and moxifloxacin-resistant Mycobacterium tuberculosis clinical isolates in southern China

Cited by 21 publications

References 48 publications

Molecular characterization of multidrug-resistant tuberculosis against levofloxacin, moxifloxacin, bedaquiline, linezolid, clofazimine, and delamanid in southwest of China

Molecular characterization of multidrug-resistant tuberculosis against levofloxacin, moxifloxacin, bedaquiline, linezolid, clofazimine, and delamanid in southwest of China

Molecular Characterization of Levofloxacin, Moxifloxacin, Bedaquiline, Linezolid, Clofazimine, and Delamanid against Multidrug-resistant Tuberculosis in Southwest of China

Effects of comprehensive nursing intervention combined with respiratory functional exercises on pulmonary function and self-care ability in patients with pulmonary tuberculosis: results of a randomized trial

Contact Info

Product

Resources

About