Sesame is one of the important oil crops in Egypt. It can be infected by fungus Fusarium oxysporum f.sp. sesami which causing wilt disease. Among totally 6 isolates of Fusarium spp. isolated from diseased sesame plants, only isolates of F. oxysporum were found to be significantly pathogenic on sesame Giza-32 cultivar and showed the same ideal wilt symptoms. The objective of this investigation was aimed to; screened 86 sesame genotypes for wilt resistance during summer seasons of 2016 and 2017 under artificial infestation and field conditions with isolate FO2. In first season, a significant differences between genotypes under study in trait of disease infection %. Ten lines were resistance i.