Phenotypic and Molecular Analysis of Oleate Content in the Mutant Soybean Line M23

Abstract

the -6 position mediated by the enzyme -6 fatty acid desaturase. Okuley et al. (1994) isolated the cDNA en-Soybean [Glycine max (L.) Merr.] oil with elevated oleate content coding the microsomal -6 fatty acid desaturase from would be useful for food and industrial applications that require increased oxidative stability. The first objective of this study was to Arabidopsis thaliana (L.) Heynh. as the Fad2 gene. Hepdetermine if molecular selection for the Fad2-1 deletion associated pard et al. (1996) found two clones encoding -6 fatty with the ol allele in the mid-oleate mutant line M23 could be used acid desaturase in soybean that they designated Fad2-1 to identify mid-oleate individuals in a breeding program. The second and Fad2-2. Heppard et al. (1996) found Fad2-1 to be objective was to determine if modifying genes affect phenotypic exstrongly expressed in developing seeds while Fad2-2 was pression of oleate content in individuals homozygous for the deleexpressed in both vegetative tissue and developing tion from a cross between M23 and Archer, a cultivar with normal seeds. oleate content. The segregation among 88 F 2 plants from the cross Kinoshita et al. (1998) used the cDNA of Fad2-1 and satisfactorily fit a ratio of 1:2:1 homozygous normal (OlOl)/heterozy-Fad2-2 as probes to screen 40 F 2 plants from the cross gous (Olol)/homozygous (olol) for the Fad2-1 deletion on the basis

Soybean Oil: Genetic Approaches for Modification of Functionality and Total Content

“…In the soybean M23 mutant, the mid-oleic acid phenotype is associated with a lesion in one of the FAD2-1 genes (Alt et al, 2005a). However, even under optimal environmental conditions, the M23 genotype and the other mid-oleic acid genotypes require pyramiding of multiple genetic alleles for full penetration of the mid-oleic acid phe- notype (Bachlava et al, 2008b;Monteros et al, 2008).…”

Phenotypic and Genomic Analyses of a Fast Neutron Mutant Population Resource in Soybean

“…Several soybean cultivars with increased oleate content are available, one of which is the midoleate mutant line M23 (Rahman et al, 1994) derived from x-ray mutagenized seed of the Bay soybean cultivar. Genetic studies indicated that the midoleate pheno- type of M23 is associated with the deletion of FAD2-1A (Alt et al, 2005;Sandhu et al, 2007;Anai et al, 2008). Given the agronomic importance of M23 in current breeding programs (Scherder et al, 2008) for increased seed oleate content, CGH could also be utilized to define the presence of other genetic lesions in the M23 genome, in addition to the previously reported FAD2-1A deletion.…”

Tocopherol Content of Soybean Lines with Mid‐oleate and 1%‐Linolenate

“…The mid-oleate content of the two MO parents, AX18896-2 and AX18895-6, was derived from M23, a mid-oleate mutant line with the ol allele developed at Saga University, Japan (Rahman et al, 1994;Takagi and Rahman, 1996;Alt et al, 2005). In additional to the ol allele, the two MO parents had, on the average, 25% of their genetic background from M23 because they were two crossing generations away from the mutant line.…”

“…Sandhu et al (2007) determined that the Fad2-1 modification producing mid-oleate content in M23 was caused by a deletion of the Fad2-1a gene and that the Fad2-1b gene was not altered. Alt et al (2005) with a range of 252 to 424 g kg -1 , and the mean for olol was 394 g kg -1 with a range of 350 to 451 g kg -1 . The overlapping ranges of oleate content among the three genotypes indicated the presence of modifying genes for oleate content.…”