Phenotypic characterization of human peripheral γδT‐cell subsets in glioblastoma

Belghali, Moulay Yassine; MOUMOU, Lahcen EL; Hazime, Raja; Brahimi, Maroua; MARRAKCHI, Malak EL; BELAID, Hasna AIT; Benali, S. Aït; Khouchani, Mouna; Ba‐M’hamed, Saadia; Admou, Brahim

doi:10.1111/1348-0421.13016

Cited by 3 publications

(2 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…CD4 + TIL from GBM contain a large fraction, up to 50%, of CD56 + T cells. Proportion of proliferating CD56 + CD4 + T cells was 3–4 times higher than fraction of proliferating CD56 − cells, and major fraction of CD56 + cells produced Th2 cytokines IL-4 and IL-13 ( Waziri et al, 2008 ; Belghali et al, 2022 ). Another study reports massive infiltration of CD4 + FoxP3 + CD25 high CD127 low regulatory T cells (Treg) in GBM and other metastatic brain tumors ( Hussain et al, 2006 ).…”

Section: Interaction Of Gbm With Immune Systemmentioning

confidence: 99%

Effects of glioblastoma-derived extracellular vesicles on the functions of immune cells

Musatova

Rubtsov

2023

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

Glioblastoma is the most aggressive variant of glioma, the tumor of glial origin which accounts for 80% of brain tumors. Glioblastoma is characterized by astoundingly poor prognosis for patients; a combination of surgery, chemo- and radiotherapy used for clinical treatment of glioblastoma almost inevitably results in rapid relapse and development of more aggressive and therapy resistant tumor. Recently, it was demonstrated that extracellular vesicles produced by glioblastoma (GBM-EVs) during apoptotic cell death can bind to surrounding cells and change their phenotype to more aggressive. GBM-EVs participate also in establishment of immune suppressive microenvironment that protects glioblastoma from antigen-specific recognition and killing by T cells. In this review, we collected present data concerning characterization of GBM-EVs and study of their effects on different populations of the immune cells (T cells, macrophages, dendritic cells, myeloid-derived suppressor cells). We aimed at critical analysis of experimental evidence in order to conclude whether glioblastoma-derived extracellular vesicles are a major factor in immune evasion of this deadly tumor. We summarized data concerning potential use of GBM-EVs for non-invasive diagnostics of glioblastoma. Finally, the applicability of approaches aimed at blocking of GBM-EVs production or their fusion with target cells for treatment of glioblastoma was analyzed.

show abstract

Section: Interaction Of Gbm With Immune Systemmentioning

confidence: 99%

Effects of glioblastoma-derived extracellular vesicles on the functions of immune cells

Musatova

Rubtsov

2023

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

show abstract

“…The proportion of total γδΤ cells in the peripheral blood of individuals with GBM was found to be similar to that of healthy individuals, but the absolute count showed a decreasing trend. Specifically, there was a decrease in double negative (CD4−CD8−) T γδ cells, an increase in immature γδΤ cells, a decrease in the expression levels of CD25 and CD279 (PD-1), and a significant increase in the expression levels of costimulatory markers CD27 and CD28 [ 58 ]. The balance between the two primary subsets, Vδ1 T cells to Vδ2 T cells, was disrupted.…”

Section: Characteristics Of γδτ Cell Expression In Patients With Gbmmentioning

confidence: 99%

Classification and function of γδT cells and its research progress in anti-glioblastoma

Zhao,

Zhu,

Wang

et al. 2023

Discov Onc

View full text Add to dashboard Cite

Human peripheral blood T lymphocytes are classified into alpha–beta T (αβΤ) cells and gamma–delta T (γδΤ) cells based on the difference in T cell receptors (TCRs). αβT cells are crucial for the acquired immune response, while γδΤ cells, though only a small subset, can recognize antigenic substances. These antigens do not need to be processed and presented and are not restricted by MHC. This distinguishes γδΤ cells from αβT cells and highlights their distinct role in innate immunity. Despite their small number, γδΤ cells hold significant significance in anti-tumor, anti-infection and immune regulation. Glioblastoma (GBM) represents one of the most prevalent malignant tumors within the central nervous system (CNS). Surgical resection alone proves to be an ineffective method for curing this type of cancer. Even with the combination of surgical resection, radiotherapy, and chemotherapy, the prognosis of some individuals with glioblastoma is still poor, and the recurrence rate is high. In this research, the classification, biological, and immunological functions of γδT cells and their research progress in anti-glioblastoma were reviewed.

show abstract

Effect of Cyclin-Dependent Kinase 4/6 Inhibitors on Circulating Cells in Patients with Metastatic Breast Cancer

Lobo-Martins,

Corredeira,

Cavaco

et al. 2024

Cells

View full text Add to dashboard Cite

The combination of cyclin-dependent kinase 4/6 inhibitors (CDK4/6i) with endocrine therapy (ET) is the standard-of-care for estrogen receptor (ER)-positive, HER2-negative (ER+/HER2− advanced/metastatic breast cancer (mBC). However, the impact of CDK4/6i on circulating immune cells and circulating tumor cells (CTCs) in patients receiving CDK4/6i and ET (CDK4/6i+ET) remains poorly understood. This was a prospective cohort study including 44 patients with ER+/HER2− mBC treated with CDK4/6i+ET in either first or second line. Peripheral blood samples were collected before (baseline) and 3 months (t2) after therapy. Immune cell’s subsets were quantified by flow cytometry, and microfluidic-captured CTCs were counted and classified according to the expression of cytokeratin and/or vimentin. Patients were categorized according to response as responders (progression-free survival [PFS] ≥ 6.0 months; 79.1%) and non-responders (PFS < 6.0 months; 20.9%). CDK4/6i+ET resulted in significant changes in the hematological parameters, including decreased hemoglobin levels and increased mean corpuscular volume, as well as reductions in neutrophil, eosinophil, and basophil counts. Specific immune cell subsets, such as early-stage myeloid-derived suppressor cells, central memory CD4+ T cells, and Vδ2+ T cells expressing NKG2D, decreased 3 months after CDK4/6i+ET. Additionally, correlations between the presence of CTCs and immune cell populations were observed, highlighting the interplay between immune dysfunction and tumor dissemination. This study provides insights into the immunomodulatory effects of CDK4/6i+ET, underscoring the importance of considering immune dynamics in the management of ER+/HER2− mBC.

show abstract

Phenotypic characterization of human peripheral γδT‐cell subsets in glioblastoma

Cited by 3 publications

References 48 publications

Effects of glioblastoma-derived extracellular vesicles on the functions of immune cells

Effects of glioblastoma-derived extracellular vesicles on the functions of immune cells

Classification and function of γδT cells and its research progress in anti-glioblastoma

Effect of Cyclin-Dependent Kinase 4/6 Inhibitors on Circulating Cells in Patients with Metastatic Breast Cancer

Contact Info

Product

Resources

About