Phenotypic Diversity among Ruminal Isolates of Prevotella ruminicola: Proposal of Prevotella brevis sp. nov., Prevotella bryantii sp. nov., and Prevotella albensis sp. nov. and Redefinition of Prevotella ruminicola

Avguštin, Gorazd; Rj, Wallace; Flint, HJ

doi:10.1099/00207713-47-2-284

Cited by 199 publications

(138 citation statements)

References 6 publications

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“…Many bacteria from the Cytophaga-Flexibacter-Bacteroides phylum are specialized in the degradation of plant fibers and proteins (4,65). The results of the clone distribution and T-RFLP and FISH analyses documented an increase in the number of bacteria from the Cytophaga-Flexibacter-Bacteroides phylum toward the posterior hindgut, which is in agreement with the accumulation of acetate, lactate, and succinate in the gut fluid of the P3 gut section of Cubitermes spp.…”

Section: Vol 69 2003 Abundance and Diversity Of Termite Gut Microorsupporting

confidence: 70%

Phylogenetic Diversity, Abundance, and Axial Distribution of Bacteria in the Intestinal Tract of Two Soil-Feeding Termites ( Cubitermes spp.)

Schmitt-Wagner

Friedrich

Wagner

et al. 2003

Appl Environ Microbiol

137

124

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The hindgut of soil-feeding termites is highly compartmentalized and characterized by pronounced axial dynamics of the intestinal pH and microbial processes such as hydrogen production, methanogenesis, and reductive acetogenesis. Nothing is known about the bacterial diversity and the abundance or axial distribution of the major phylogenetic groups in the different gut compartments. In this study, we showed that the variety of physicochemical conditions is reflected in the diversity of the microbial communities in the different gut compartments of two Cubitermes species (Termitidae: Termitinae). 16S rRNA gene clones from the highly alkaline first proctodeal segment (

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Section: Vol 69 2003 Abundance and Diversity Of Termite Gut Microorsupporting

confidence: 70%

Phylogenetic Diversity, Abundance, and Axial Distribution of Bacteria in the Intestinal Tract of Two Soil-Feeding Termites ( Cubitermes spp.)

Schmitt-Wagner

Friedrich

Wagner

et al. 2003

Appl Environ Microbiol

137

124

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“…This value lies within the range reported for the species in the genus Prevotella (39-60 mol%) (Avgustin, 1997, Willems & Collins, 1995. Compared with the phylogenetically most closely related species, strain A1336 T can clearly be distinguished by its genomic DNA G+C content from P. bergensis [48 mol%; (Downes et al, 2006)] and P. multisaccharivorax [49.9 mol%; (Sakamoto et al, 2005)].…”

supporting

confidence: 79%

Prevotella colorans sp. nov., isolated from a human wound

Buhl

Willmann

Liese

et al. 2016

International Journal of Systematic and Evolutionary Microbiology

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A strain of obligately anaerobic, Gram-stain-negative and non-spore-forming rod-shaped bacterium was isolated from a human wound and characterized both phenotypically and genotypically. The strain was moderately saccharolytic and proteolytic. Phylogenetic analysis was based on full-length 16S rRNA gene sequence analysis and revealed the strain to represent a member of the genus Prevotella, but to be different from the described species, with the closest relationship to Prevotella bergensis and Prevotella multisaccharivorax. The genomic DNA G+C content was 43.2 mol%. The most abundant cellular long-chain fatty acids were 3-OH iso-C 17 : 0 , anteiso-C 15 : 0 and iso-C 15 : 0 . In view of phenotypical and biochemical characteristics as well as gene sequencing, strain A1336T is considered to represent a novel species within the genus

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“…Members of Prevotellaceae utilize various substrates, such as starch, protein, peptides, hemicellulose, and pectin, to generate a wide range of end products, mainly short-chain fatty acids, including acetate, succinate, and propionate (3,5). This bacterial family has a remarkable degree of genetic diversity (28,29) and occupies various ecological niches within the rumen (25). Our results provide further understanding regarding its consistent presence in the rumen, especially with animals fed a barley-based high-grain diet.…”

Section: Discussionmentioning

confidence: 99%

Metatranscriptomic Profiling Reveals Linkages between the Active Rumen Microbiome and Feed Efficiency in Beef Cattle

Guan

2017

Appl Environ Microbiol

268

216

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Exploring compositional and functional characteristics of the rumen microbiome can improve the understanding of its role in rumen function and cattle feed efficiency. In this study, we applied metatranscriptomics to characterize the active rumen microbiomes of beef cattle with different feed efficiencies (efficient, n ϭ 10; inefficient, n ϭ 10) using total RNA sequencing. Active bacterial and archaeal compositions were estimated based on 16S rRNAs, and active microbial metabolic functions including carbohydrate-active enzymes (CAZymes) were assessed based on mRNAs from the same metatranscriptomic data sets. In total, six bacterial phyla (Proteobacteria, Firmicutes, Bacteroidetes, Spirochaetes, Cyanobacteria, and Synergistetes), eight bacterial families (Succinivibrionaceae, Prevotellaceae, Ruminococcaceae, Lachnospiraceae, Veillonellaceae, Spirochaetaceae, Dethiosulfovibrionaceae, and Mogibacteriaceae), four archaeal clades (Methanomassiliicoccales, Methanobrevibacter ruminantium, Methanobrevibacter gottschalkii, and Methanosphaera), 112 metabolic pathways, and 126 CAZymes were identified as core components of the active rumen microbiome. As determined by comparative analysis, three bacterial families (Lachnospiraceae, Lactobacillaceae, and Veillonellaceae) tended to be more abundant in low-feed-efficiency (inefficient) animals (P Ͻ 0.10), and one archaeal taxon (Methanomassiliicoccales) tended to be more abundant in high-feed-efficiency (efficient) cattle (P Ͻ 0.10). Meanwhile, 32 microbial metabolic pathways and 12 CAZymes were differentially abundant (linear discriminant analysis score of Ͼ2 with a P value of Ͻ0.05) between two groups. Among them, 30 metabolic pathways and 11 CAZymes were more abundant in the rumen of inefficient cattle, while 2 metabolic pathways and 1 CAZyme were more abundant in efficient animals. These findings suggest that the rumen microbiomes of inefficient cattle have more diverse activities than those of efficient cattle, which may be related to the host feed efficiency variation.IMPORTANCE This study applied total RNA-based metatranscriptomics and showed the linkage between the active rumen microbiome and feed efficiency (residual feed intake) in beef cattle. The data generated from the current study provide fundamental information on active rumen microbiome at both compositional and functional levels, which serve as a foundation to study rumen function and its role in cattle feed efficiency. The findings that the active rumen microbiome may contribute to variations in feed efficiency of beef cattle highlight the possibility of enhancing nutrient utilization and improve cattle feed efficiency through modification of rumen microbial functions.KEYWORDS metatranscriptome, RNA-seq, rumen microbiome, beef cattle, feed efficiency R uminants can convert fibrous plant materials into edible meat and milk products for human consumption through rumen microbial fermentation. The symbiotic rumen microbiota mainly consists of bacteria, archaea, fungi, and ciliated protozoa (1)

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Phenotypic Diversity among Ruminal Isolates of Prevotella ruminicola: Proposal of Prevotella brevis sp. nov., Prevotella bryantii sp. nov., and Prevotella albensis sp. nov. and Redefinition of Prevotella ruminicola

Cited by 199 publications

References 6 publications

Phylogenetic Diversity, Abundance, and Axial Distribution of Bacteria in the Intestinal Tract of Two Soil-Feeding Termites ( Cubitermes spp.)

Phylogenetic Diversity, Abundance, and Axial Distribution of Bacteria in the Intestinal Tract of Two Soil-Feeding Termites ( Cubitermes spp.)

Prevotella colorans sp. nov., isolated from a human wound

Metatranscriptomic Profiling Reveals Linkages between the Active Rumen Microbiome and Feed Efficiency in Beef Cattle

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