Phenotypic effect of a single nucleotide polymorphism on SSC7 on fetal outcomes in PRRSV-2 infected gilts

Ko, Haesu; Sammons, Jourdyn; Pasternak, J. Alex; Hamonic, Glenn; Starrak, Gregory; MacPhee, Daniel J.; Detmer, Susan E.; Plastow, Graham; Harding, John C. S.

doi:10.1016/j.livsci.2021.104800

Cited by 8 publications

(18 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Fetal body weights were recorded post-blood collection, and morphometric images and analysis were done similar to that reported in Ko et al. [ 39 ]. In short, each fetus was imaged in left and right lateral recumbence using a fixed and level camera, with calibration chips included in each image.…”

Section: Methodsmentioning

confidence: 99%

“…However, three meconium-stained fetuses were chosen because of a lack of viable fetuses within a uterine horn. Blood was collected from the axillary artery of all fetuses immediately after removal from the maternal tract, and serum was later separated and stored at −20 • C. Fetal body weights were recorded postblood collection, and morphometric images and analysis were done similar to that reported in Ko et al [39]. In short, each fetus was imaged in left and right lateral recumbence using a fixed and level camera, with calibration chips included in each image.…”

Section: Blood and Tissue Collectionmentioning

confidence: 99%

See 1 more Smart Citation

Compensatory mechanisms in response to induced hypothyroidism in the late gestation pig fetus

Ison

Kent-Dennis

Fazioli

et al. 2023

Biology of Reproduction

View full text Add to dashboard Cite

To understand the effect of fetal thyroid gland disruption on development in swine, we evaluated thyroid hormone levels, growth and developmental characteristics, and gene expression associated with thyroid hormone metabolism in late gestation fetuses exposed to methimazole (MMI). Pregnant gilts were given either oral MMI or equivalent sham from gestation day 85–106 (n = 4/group), followed by intensive phenotyping of all fetuses (n = 120). Samples of liver (LVR), kidney (KID), fetal placenta (PLC) and the corresponding maternal endometrium (END) were collected from a subset of fetuses (n = 32). Fetuses exposed to MMI in utero were confirmed hypothyroid, with a significant increase in thyroid gland size, goitrous thyroid histology, and dramatically suppressed thyroid hormone in serum. In the dams, no differences in temporal measurements of average daily gain, thyroid hormone, or rectal temperatures relative to controls suggest that MMI had little effect on maternal physiology. However, fetuses from MMI treated gilts exhibited significant increases in body mass, girth, and vital organ weights, but no differences in crown-rump length or bone measurements suggest non-allometric growth. The PLC and END showed a compensatory decrease in expression of inactivating deiodinase (DIO3). Similar compensatory gene expression was observed in fetal KID and LVR with a downregulation of all deiodinases (DIO1, DIO2, DIO3). Minor alterations in the expression of thyroid hormone transporters (SLC16A2 and SLC16A10) were observed in PLC, KID and LVR. Collectively, MMI crosses the fetal placenta of the late gestation pig, resulting in congenital hypothyroidism, alterations in fetal growth, and compensatory responses within the maternal fetal interface.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Blood and Tissue Collectionmentioning

confidence: 99%

Compensatory mechanisms in response to induced hypothyroidism in the late gestation pig fetus

Ison

Kent-Dennis

Fazioli

et al. 2023

Biology of Reproduction

View full text Add to dashboard Cite

show abstract

“…The value in this measure is derived from an established ratio of production (1:13) in the healthy thyroid, however such a ratio has not been effectively established in a healthy fetal pig throughout gestation. The second stems from our past investigations into fetal thyroid hormone response to PRRSV ( Pasternak et al, 2020b ; Ko et al, 2022 ) which indicates that the relative response of these two hormones is dependent on fetal phenotype. In short fetuses classified as viable show a decrease in both T3 and T4 while those classified as meconium stained primarily have a decrease in T4 while maintaining near normal levels of T3.…”

Section: Methodsmentioning

confidence: 99%

“…Severe illness can result in a dramatic decrease in serum T3 and T4 levels, collectively called non-thyroidal illness syndrome (NTIS) ( Wajner and Maia, 2012 ). Previous research has shown that piglet and fetal serum thyroid hormone (i.e., T3 and/or T4) levels decrease rapidly in response to PRRSV infection ( Pasternak et al, 2020b ; Pasternak et al, 2021 ; Ko et al, 2022 ). Recent assessment of fetal thyroid hormone levels following late gestation PRRSV challenge demonstrated a significant relationship between hormone levels and previously identified single nucleotide polymorphisms (SNPs) associated with fetal survival ( Ko et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

“…Previous research has shown that piglet and fetal serum thyroid hormone (i.e., T3 and/or T4) levels decrease rapidly in response to PRRSV infection ( Pasternak et al, 2020b ; Pasternak et al, 2021 ; Ko et al, 2022 ). Recent assessment of fetal thyroid hormone levels following late gestation PRRSV challenge demonstrated a significant relationship between hormone levels and previously identified single nucleotide polymorphisms (SNPs) associated with fetal survival ( Ko et al, 2022 ). However, the genetic control of T3 and T4 levels during infection is not completely understood.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Genome wide association study of thyroid hormone levels following challenge with porcine reproductive and respiratory syndrome virus

Goor

Pasternak²,

Walugembe³

et al. 2023

Front. Genet.

Self Cite

View full text Add to dashboard Cite

Introduction: Porcine reproductive and respiratory syndrome virus (PRRSV) causes respiratory disease in piglets and reproductive disease in sows. Piglet and fetal serum thyroid hormone (i.e., T3 and T4) levels decrease rapidly in response to Porcine reproductive and respiratory syndrome virus infection. However, the genetic control of T3 and T4 levels during infection is not completely understood. Our objective was to estimate genetic parameters and identify quantitative trait loci (QTL) for absolute T3 and/or T4 levels of piglets and fetuses challenged with Porcine reproductive and respiratory syndrome virus.Methods: Sera from 5-week-old pigs (N = 1792) at 11 days post inoculation (DPI) with Porcine reproductive and respiratory syndrome virus were assayed for T3 levels (piglet_T3). Sera from fetuses (N = 1,267) at 12 or 21 days post maternal inoculation (DPMI) with Porcine reproductive and respiratory syndrome virus of sows (N = 145) in late gestation were assayed for T3 (fetal_T3) and T4 (fetal_T4) levels. Animals were genotyped using 60 K Illumina or 650 K Affymetrix single nucleotide polymorphism (SNP) panels. Heritabilities, phenotypic correlations, and genetic correlations were estimated using ASREML; genome wide association studies were performed for each trait separately using Julia for Whole-genome Analysis Software (JWAS).Results: All three traits were low to moderately heritable (10%–16%). Phenotypic and genetic correlations of piglet_T3 levels with weight gain (0–42 DPI) were 0.26 ± 0.03 and 0.67 ± 0.14, respectively. Nine significant quantitative trait loci were identified for piglet_T3, on Sus scrofa chromosomes (SSC) 3, 4, 5, 6, 7, 14, 15, and 17, and collectively explaining 30% of the genetic variation (GV), with the largest quantitative trait loci identified on SSC5, explaining 15% of the genetic variation. Three significant quantitative trait loci were identified for fetal_T3 on SSC1 and SSC4, which collectively explained 10% of the genetic variation. Five significant quantitative trait loci were identified for fetal_T4 on SSC1, 6, 10, 13, and 15, which collectively explained 14% of the genetic variation. Several putative immune-related candidate genes were identified, including CD247, IRF8, and MAPK8.Discussion: Thyroid hormone levels following Porcine reproductive and respiratory syndrome virus infection were heritable and had positive genetic correlations with growth rate. Multiple quantitative trait loci with moderate effects were identified for T3 and T4 levels during challenge with Porcine reproductive and respiratory syndrome virus and candidate genes were identified, including several immune-related genes. These results advance our understanding of growth effects of both piglet and fetal response to Porcine reproductive and respiratory syndrome virus infection, revealing factors associated with genomic control of host resilience.

show abstract

Effect of porcine reproductive and respiratory syndrome virus 2 on tight junction gene expression at the maternal-fetal interface

et al. 2022

View full text Add to dashboard Cite

Phenotypic effect of a single nucleotide polymorphism on SSC7 on fetal outcomes in PRRSV-2 infected gilts

Cited by 8 publications

References 53 publications

Compensatory mechanisms in response to induced hypothyroidism in the late gestation pig fetus

Compensatory mechanisms in response to induced hypothyroidism in the late gestation pig fetus

Genome wide association study of thyroid hormone levels following challenge with porcine reproductive and respiratory syndrome virus

Effect of porcine reproductive and respiratory syndrome virus 2 on tight junction gene expression at the maternal-fetal interface

Contact Info

Product

Resources

About