Phenotypic expression of parasite susceptibility to Haemonchus contortus in Pelibuey sheep

Ojeda-Robertos, Nadia Florencia; Torres-Acosta, J.F.J.; González-Gardúño, Roberto; Notter, D. R.

doi:10.1016/j.vetpar.2017.04.015

Cited by 12 publications

(7 citation statements)

References 23 publications

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“…Goats that had lower FEC, indicating greater resistance to infection, under natural grazing conditions appeared much more resistant, based on indicators of parasite resistance, and also had greater expression of five of the six candidate genes in response to a subsequent controlled GIN infection. The high repeatability for indicators of parasite resistance across infection cycles was consistent with results reported for Pelibuey hair sheep [63], and differences in expression of genes involved in immune function have been reported between resistant and susceptible sheep breeds [64]. The results of present study show that the majority of candidate genes selected for this study were differentially expressed in resistant and susceptible goats, further supporting the potential value of SNP in these genes as markers for GIN resistance in goats.…”

Section: Discussionsupporting

confidence: 87%

Single nucleotide polymorphisms in candidate genes are significantly associated with resistance to Haemonchus contortus infection in goats

Alam

Omar

Faruque

et al. 2019

J Animal Sci Biotechnol

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BackgroundHaemonchosis is a major economic problem in goat production in humid, tropical and subtropical regions. The disease is caused by an abomasal nematode, Haemonchus contortus, which is highly pathogenic in small ruminants. The aim of this study was to identifying single-nucleotide polymorphisms (SNP) that were associated with fecal egg counts (FEC) and could be used as markers to identify resistance to H. contortus in goats.ResultsTen novel variants in the CIITA, ATP2A3, HSPA8, STAT5B, ESYT1, and SERPING1 genes were associated with FEC in goats with a nominal significance level of P < 0.05. Two missense mutation in the exon region of the caprine CIITA gene resulted in replacement of arginine with cysteine at position 9473550 (R9473550C) and aspartic acid with glutamic acid at position 9473870 (D9473870E). Chinese goat breeds had significantly higher FEC than Bangladeshi goat breeds within their respective genotypes. Polymorphism information content (PIC), effective allele number (Ne), and heterozygosity (He) were greatest for the STAT5B_197_A > G SNP locus in all goat breeds. Pairwise coefficients of linkage disequilibrium (D´, r2) revealed complete LD (r2 = 1) between significant SNP polymorphisms in CIITA and SERPING1 and strong LD (r2 = 0.93 and 0.98) between polymorphisms in HSPA8 and ATP2A3, respectively. Correlation coefficient (r) between FEC and body weight (BW) was significantly positive (r = 0.56***, P < 0.001) but that between FEC and packed cell volume (PCV) was negatively significant (r = − 0.47**, P < 0.01) in the total population of goats. On the other hand, correlation coefficient (r) between BW and PCV was not significant in total population of goats. Association analysis revealed that haplotypes within ATP2A3, HSPA8, and SERPING1 were significantly associated with FEC. Quantitative real-time PCR revealed that the relative expression of mRNA was higher (P < 0.001) for resistant, compared to susceptible, groups of goats for all candidate genes except CIITA.ConclusionsThis study identified SNP markers that can potentially be used in marker-assisted selection programs to develop goat breeds that are resistant to H. contortus.Electronic supplementary materialThe online version of this article (10.1186/s40104-019-0327-8) contains supplementary material, which is available to authorized users.

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Section: Discussionsupporting

confidence: 87%

Single nucleotide polymorphisms in candidate genes are significantly associated with resistance to Haemonchus contortus infection in goats

Alam

Omar

Faruque

et al. 2019

J Animal Sci Biotechnol

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“…Filtration was made to avoid debris, and eggs were collected by sedimentation process using slow centrifugation at 100 rpm for 2 min. Coprocultures were maintained under laboratory conditions (with 25 0 C and 80% humidity) and moistened and aerated daily for eight days (Ojeda-Robertos et al 2017). A culture of third-stage (infective stage) larvae (L 3 ) in vitro condition was recovered following the slandered Baerman technique (Hansen & Perry 1994;Ojeda-Robertos et al 2017).…”

Section: Experimental Sitementioning

confidence: 99%

“…Coprocultures were maintained under laboratory conditions (with 25 0 C and 80% humidity) and moistened and aerated daily for eight days (Ojeda-Robertos et al 2017). A culture of third-stage (infective stage) larvae (L 3 ) in vitro condition was recovered following the slandered Baerman technique (Hansen & Perry 1994;Ojeda-Robertos et al 2017). Harvested larvae were counted in each of five 20ul aliquots, and larval density (larvae/ml) was estimated.…”

Section: Experimental Sitementioning

confidence: 99%

“…Eight goats (4 resistant and 4 susceptible ) were selected to dry lot for challenge trial and treated with broad-spectrum anthelmintic viz., Ivermectin (0.25 mg/kg body weight) and 15 days later, with levamisole (8 mg/kg body weight) to ensure the animals were completely dewormed (Bambou et al 2013; Kurukulasuriya et al 2018). In dry lot, each goat were fed 30 g/kg BW of balance feed including GINfree fresh grass per day on a dry-matter basis, and a concentrated mixture was provided twice per day at a rate of 1.5% of BW containing 18% crude protein and 10.6UJ/kg of metabolizable energy on a dry matter basis (Ojeda-Robertos et al 2017). When all experimental goats achieved a FEC of zero ensured by microscopic examination, resistant and susceptible goats were inoculated with a single dose of 5,000 thirdstage (L3) larvae of H. contortus (Notter et al 2003;Getachew et al 2015;Kurukulasuriya et al 2018).…”

Section: Nematode Challenge Trialmentioning

confidence: 99%

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Assessment of genetic resistance and abomasal tissues expression of Yichang white goat experimentally challenge with Haemonchus contortus infection

et al. 2019

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Our previous association study revealed the mutation in candidate immune genes (NOD1 & NLRP9) was significantly associated with FEC of Haemonchus contortus infection in Yichang white goats, but the relative expression of mRNA of those genes associated with resistance to H. contortus was not investigated. Aim of the current experiment was to evaluate the susceptible and resistant individuals to nematode infection within the population of Yichang white goat (YWG) and assess the differential level of mRNA expression of those candidate genes in the abomasal tissues of susceptible and resistant goats. Fecal egg count (FEC) was determined using a modified McMaster technique, and the hematological parameter was measured by Mindray auto hematology analyzer. Phenotype data were collected and analyzed using a generalized linear model with SAS statistical program. Field investigate revealed that the prevalence (76%) with maxium parasite load (734.34±84.21epg) of H. contortus occurred in August within the experimental flock FEC in resistant group (103.38±1.20epg) and susceptible group (1180.25±43.53epg) group demonstrated the presence of two distinct goat populations within this breed. Four resistant and four susceptible goats were selected from each group. The parasite infection was established by artificially challenge with 5000 infective L3 larvae of H. contortus. Abomasal tissues were collected from all experimental goats after 42 days of post-infection. FEC, Body weight, packed cell volume, and hemoglobin value were significantly different (P < 0.01) between resistant and susceptible group of goats. Quantitative real-time PCR in abomasal tissues revealed that the expression level of mRNA for NOD1 (P < 0.00001), IFNG (P < 0.0001), NLRP9, TLR8, IL32, (P < 0.001) and IGF1 (P < 0.01) was higher in resistant goat compared to susceptible, except SFTPA1. These findings revealed the presence of genetic resistant individuals to H. contortus within the goat breed and expression of NOD1 and NLRP9 genes proved the positive finding of our previous study. Presence of genetic resistant individuals in Yichang white goat YWG breed could be a good candidate for selective breeding and highly expressed genes related to resistant could be used as biomarkers to develop H. contortus resistant goat population. Progressive Agriculture 30 (2): 194-208, 2019

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“…With many variants, oral challenge of lambs with infective larvae (L3) and their subsequent challenge with either artificial or natural infection is the most widely used protocol (Saccareau et al ., 2017), where the level of antigenic stimulus is an important event in the speed of acquisition of acquired immunity. In a previous study, it is reported that in Pelibuey lambs initially infected orally with 7500 L3 of H. contortus , it was possible to successfully segregate RES and susceptible (SUS) individuals at the end of that infection according to their average FEC (Ojeda-Robertos et al ., 2017).…”

Section: Introductionmentioning

confidence: 99%

Artificial Haemonchus contortus infection as a strategy to induce protective immune response to natural infection in Pelibuey lambs

et al. 2020

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The objective of this study was to evaluate the reduction in nematode faecal egg count (FEC) in Pelibuey lambs segregated as resistant (RES), susceptible (SUS) and intermediate (INT) to gastrointestinal nematodes. Twenty-nine weaned Pelibuey lambs, aged five months old, free of nematode infection, were used. Nine lambs were RES, six were SUS and 14 were INT lambs. The study consisted of two phases: in Phase 1 the lambs were infected experimentally with Haemonchus contortus. In Phase 2, the lambs were naturally infected by grazing. Faecal and blood samples were taken every week. The packed cell volume and total protein were quantified. The FEC value (FECmax) per lamb was recorded together with a natural reduction in FEC in the two phases. The data were analysed with a model of measures repeated over time. During Phase 1, the RES lambs showed the lowest FEC (1061 ± 1053) compared to the other groups (INT: 2385 ± 1794 eggs per gram of faeces (EPG); and SUS: 3958 ± 3037 EPG). However, in Phase 2 no significant differences (p > 0.05) were observed between the groups of lambs (RES: 275 ± 498 EPG; SUS: 504 ± 1036 EPG; and INT: 603 ± 1061 EPG). At the end of Phase 1, the FEC of RES lambs was naturally reduced by 75.5% in respect to FECmax (p < 0.05), and at the end of Phase 2 the reduction in FEC was 90% in respect to FECmax (p > 0.05); the same behaviour was observed in RES and SUS lambs. It is concluded that the artificial infection in the lambs induced a more rapid immune response in RES than SUS lambs, and all lambs developed high acquired resistance by continuous infection.

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Phenotypic expression of parasite susceptibility to Haemonchus contortus in Pelibuey sheep

Cited by 12 publications

References 23 publications

Single nucleotide polymorphisms in candidate genes are significantly associated with resistance to Haemonchus contortus infection in goats

Single nucleotide polymorphisms in candidate genes are significantly associated with resistance to Haemonchus contortus infection in goats

Assessment of genetic resistance and abomasal tissues expression of Yichang white goat experimentally challenge with Haemonchus contortus infection

Artificial Haemonchus contortus infection as a strategy to induce protective immune response to natural infection in Pelibuey lambs

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