Phenotypic, genetic and expression profiling of a vindoline-rich genotype of Catharanthus roseus

Mall, Maneesha; Singh, Pooja; Kumar, Ritesh; Shanker, Karuna; Gupta, Anil Kumar; Khare, Puja; Shasany, Ajit Kumar; Khatoon, Sayyada; Sundaresan, Velusamy; Baskaran, K.; Yadav, Seema; Shukla, Ashutosh K.

doi:10.1016/j.sajb.2021.02.004

Cited by 6 publications

(3 citation statements)

References 34 publications

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“…Analogously, it has been postulated that intrusive growth of laticifers may also be affected by enzymes like PME through pectin modification (Hagel et al, 2008). In case of C. roseus , the results obtained in our present and previous (Mall et al, 2021) studies seem to support this hypothesis. The pattern of CrPME transcript abundance and PME specific activity in the three familial C. roseus genotypes was similar to the pattern of laticifer number and vindoline (laticifer‐specific TIA) content in them ‐ CIM‐Sushil > Dhawal > Nirmal.…”

Section: Discussionsupporting

confidence: 88%

“…Experimental plants were kept in a glasshouse under standard conditions (25 ± 2°C, 16/8 h light/dark photoperiod, 60–75% relative humidity). The leaf vindoline content (% dry weight basis) of the three familial genotypes was in the order: CIM‐Sushil (~0.20%) > Dhawal (0.09%) > Nirmal (0.04%) (Gupta et al, 2020; Mall et al, 2021). For the comparative experiments (for PME protein activity and gene expression), all three genotypes were used, whereas all other experiments were carried out on the Dhawal genotype.…”

Section: Methodsmentioning

confidence: 99%

“…The primordia of such laticifers are found in the developing shoot beneath the apical meristems and grow into unbranched tubes through a combination of intrusive and coordinated growth (Evert, 2006). Comparative analysis of familial C. roseus genotypes differing in their vindoline content indicated that vindoline content is a direct function of laticifer number (Mall et al, 2021). It has also been shown that vindoline accumulation increases in laticifers with leaf expansion (Yamamoto et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

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Characterization of a CrPME indicates its possible role in determining vindoline accumulation in Catharanthus roseus leaves

Singh,

Yadav,

Shah

et al. 2024

Physiologia Plantarum

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The leaf‐specific Catharanthus roseus alkaloid, vindoline, is the major bottleneck precursor in the production of scarce and costly anticancer bisindoles (vincristine and vinblastine). The final steps of its biosynthesis and storage occur in the laticifers. Earlier, we have shown that vindoline content is directly related to laticifer number. Pectin remodeling enzymes, like pectin methylesterase (PME), are known to be involved in laticifer development. A search in the croFGD yielded a leaf‐abundant CrPME isoform that co‐expressed with a few vindoline biosynthetic genes. Full‐length cloning, tissue‐specific expression profiling, and in silico analysis of CrPME were carried out. It was found to possess all the specific characteristics of a typical plant PME. Transient silencing (through VIGS) and overexpression of CrPME in C. roseus indicated a direct relationship between its expression and vindoline content. Comparative analysis of transcript abundance and enzyme activity in three familial C. roseus genotypes differing significantly in their vindoline content and laticifer count (CIM‐Sushil > Dhawal > Nirmal) also corroborated the positive relationship of CrPME expression with vindoline content. This study highlights the possible role of CrPME, a cell wall remodeling enzyme, in modulating laticifer‐associated secondary metabolism.

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Section: Discussionsupporting

confidence: 88%

Section: Methodsmentioning

confidence: 99%