Phenotypic, Genetic, and Metabolite Variability among Genotypes of Vicia sativa L.

Abstract

Vicia sativa L., commonly known as the common vetch, is an annual, self-pollinating legume used primarily as fodder both by livestock and wildlife. Additionally, it contributes to environmental balance through nitrogen fixation and the improvement of soil properties. The phenotypic, genetic, and metabolite variability among four advanced lines (BK45, BK29, BK23, BK27) and two commercial varieties (M-6900, BI-65) of V. sativa were evaluated in order to be used for future breeding programs aimed at producing genetically improved varieties. BK45 was the most promising line due to its high genetic polymorphism, but also because it exhibited a significant amount of seed production and high seed quality based on its metabolomics profile. A stepwise multiple regression analysis (MRA) revealed a relationship between SCoT alleles, seed, and biomass yield. Additionally, several statistically significant marker bands linked to metabolites were found using the SCoT marker analysis. Hence, data assessed via MRA may be helpful in marker-assisted breeding programs. Finally, the two commercial varieties can be further exploited in breeding programs due to their high genetic diversity.