Phenotypic, Physiological, and Molecular Evaluation of Rice Chilling Stress Response at the Vegetative Stage

Reyes, Benildo G. de los; Yun, Song Joong; Herath, Venura; Xu, Fuyu; Park, Myoung Ryoul; Lee, Jeong Il; Kim, Ki Young

doi:10.1007/978-1-62703-194-3_16

Cited by 13 publications

(17 citation statements)

References 19 publications

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“…Oxidative damage is the primary contributor to cold injuries in plants (de Los Reyes et al, 2013). Therefore, the MDA content and ion leakage were measured as indictors of lipid peroxidation and direct cell damage.…”

Section: Overexpression Of Osaox1a Results In Less Oxidative Damage Umentioning

confidence: 99%

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Overexpression of an alternative oxidase gene, OsAOX1a, improves cold tolerance in Oryza sativa L.

Li¹,

Liang²,

Xu³

et al. 2013

Genet. Mol. Res.

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ABSTRACT. Low temperature is a major environmental stress in rice cultivating and production. The alternative oxidase 1 (AOX1) gene is potentially important for genetic engineering to increase cold adaptation. However, previous studies related to this effect have mostly focused on the dicot plants Arabidopsis and tobacco, whereas functional research on rice is limited. In this study, we cloned a rice predominant cold-response AOX1 gene, OsAOX1a. Transgenic rice plants with overexpression of OsAOX1a were obtained. We found that OsAOX1a overexpression could strongly enhance the cold growth of seedlings, especially with respect to root extension. However, growth between transgenic and control plants did not differ under normal conditions. Furthermore, the lipid peroxidation and ion leakage rate were determined after cold treatment in transgenic plants. Both factors were reduced by OsAOX1a OsAOX1a overexpression enhanced cold tolerance overexpression, which revealed that OsAOX1a could reduce oxidative damage under cold stress. Taken together, our results suggested that overexpressing OsAOX1a could improve growth performance of rice under cold stress, which might be closely related to the reduction of reactive oxygen species generation and oxidative damage.

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Section: Overexpression Of Osaox1a Results In Less Oxidative Damage Umentioning

confidence: 99%

“…Cold exposure at the seedling and subsequent vegetative growth stages can lead to poor germination, yellowing leaves, and growth retardation (de Los Reyes et al, 2013). Unpredictable chilling snaps at the reproductive stage causes heading delay and high sterility, resulting in severe yield losses (Jena et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Overexpression of an alternative oxidase gene, OsAOX1a, improves cold tolerance in Oryza sativa L.

Li¹,

Liang²,

Xu³

et al. 2013

Genet. Mol. Res.

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show abstract

“…Plants have evolved diverse mechanisms in response to chilling and freezing temperatures (Zhu et al 2007). Although some studies on chilling or cold shock responses have been reported (Chaikam & Karlson 2008;Chawade et al 2013;de Los Reyes et al 2013;Mao & Chen 2012;Yang et al 2013a), the differences between the genetic basis of chilling and cold tolerance were seldom addressed. To the best of our knowledge, this study was the first attempt to address the difference in genetic basis of chilling and cold shock stress tolerance.…”

Section: Distinct Genetic Basis Of Natural Chilling and Cold Shock Tomentioning

confidence: 99%

“…The ecogeographical differentiation of the two subspecies has been reported (Londo et al 2006;Yu et al 2003). It has been generally accepted that japonica rice had higher potential in cold adaptability than indica rice (Cheng et al 2007;de Los Reyes et al 2013;Lu et al 2014;Ma et al 2015;Morsy et al 2005;Pan et al 2015), but the genetic evidence for this is very limited.…”

Section: Introductionmentioning

confidence: 99%

New insights into the genetic basis of natural chilling and cold shock tolerance in rice by genome‐wide association analysis

Guo

et al. 2015

Plant Cell & Environment

119

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In order to understand cold adaptability and explore additional genetic resources for the cold tolerance improvement of rice, we investigated the genetic variation of 529 rice accessions under natural chilling and cold shock stress conditions at the seedling stage using genome-wide association studies; a total of 132 loci were identified. Among them, 12 loci were common for both chilling and cold shock tolerance, suggesting that rice has a distinct and overlapping genetic response and adaptation to the two stresses. Haplotype analysis of a known gene OsMYB2, which is involved in cold tolerance, revealed indica-japonica differentiation and latitude tendency for the haplotypes of this gene. By checking the subpopulation and geographical distribution of accessions with tolerance or sensitivity under these two stress conditions, we found that the chilling tolerance group, which mainly consisted of japonica accessions, has a wider latitudinal distribution than the chilling sensitivity group. We conclude that the genetic basis of natural chilling stress tolerance in rice is distinct from that of cold shock stress frequently used for low-temperature treatment in the laboratory and the cold adaptability of rice is associated with the subpopulation and latitudinal distribution.

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“…The procedures of Ballou et al (2007) was adopted to determine the % electrolyte leakage from the L1, L2, and L3 leaf discs by measuring the electrical conductivity (EC) in 2 ml of pure nanopure water (16 to18 M∧) before and after stress using a Fisherbrand TM Traceable TM conductivity meter (Thermo Scientific, Waltham, MA, USA). The %EL from intact tissues was determined relative to total tissue electrolytes after boiling, expressed either %EL or electrolyte leakage index (ELI), according to de los Reyes et al (2012).…”

Section: Methodsmentioning

confidence: 99%

Physiological networks governing salinity tolerance potentials inGossypium hirsutumgermplasm

Cushman

Pabuayon

Hinze

et al. 2019

Preprint

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6 7 8 Physiological networks governing salinity tolerance potentials in 9Gossypium hirsutum germplasm 10 One-Sentence Summary: 17Variation in salinity tolerance potential across the tetraploid cultivated 18Gossypium germplasm is better explained by complex physiological networks 19 rather than just cellular Na + homeostasis. 20 1 KRC performed the research, designed the experiment, analyzed the data, co-wrote the manuscript with BGDR; 1 ICMP contributed to the gene expression experiments; 2 LLH assisted in germplasm selection from the GDRS; 3 MES assisted in the design of physiological experiments; 1 BGDR conceptualized the whole project, designed the experiment and wrote the manuscript. ABSTRACT: 21Toxic ions begin to accumulate in tissues of salt-stressed plants after the 22 initial osmotic shock. In glycophytes, the ability to mobilize or sequester excess 23 ions define tolerance mechanisms. Mobilization and sequestration of excess Na + 24 involves three transport mechanisms facilitated by the plasma membrane H + /Na + 25 antiporter (SOS1), vacuolar H + /Na + antiporter (NHX1), and Na + /K + transporter in 26 vascular tissues (HKT1). While the cultivated Gossypium hirsutum (upland 27 cotton) is significantly more tolerant to salinity relative to other crops, the critical 28 factors contributing to the observed variation for tolerance potential across the 29 germplasm has not been fully scrutinized. In this study, the spatio-temporal 30 patterns of Na + accumulation at different severities of salt stress were 31 investigated across a minimal comparative panel representing the spectrum of 32 genetic diversity across the improved cotton germplasm. The goal was to define 33 the importance of integrative or network effects relative to the direct effects of 34 Na + homeostasis mechanisms mediated by GhHKT1, GhSOS1, and GhNHX1. 35 Multi-dimensional physio-morphometric attributes were investigated in univariate 36 and multivariate statistical contexts, as well as the relationship between variables 37 using structural equation modeling. Results showed that mobilized or 38 sequestered Na + may contribute to the baseline salinity tolerance, but the 39 observed variance in overall tolerance potential across a meaningful subset of 40 the germplasm were more significantly associated to antioxidant capacity, 41 maintenance of stomatal conductance, chlorophyll content, and divalent cations, 42and other physiological interactions occurring through complex networks. reduction of long distance Na + transport facilitated by HKT1 and SOS1, and by 67 efficient sequestration of Na + into the root vacuoles through NHX1 and AVP 68 mediated mechanisms (Cheeseman, 1988; Apse et al., 1999; Blumwald et al., 69 2000; Halfter et al., 2000; Maser et al., 2002; Chinnusamy et al., 2004; Haro et 70 al., 2005; Brini et al., 2007). 71Numerous studies in Arabidopsis and several crop plants have shown that 72 positive net gains in salinity tolerance can be achieved with the overexpression of 73 critical genes involved in Na + transport an...

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Phenotypic, Physiological, and Molecular Evaluation of Rice Chilling Stress Response at the Vegetative Stage

Cited by 13 publications

References 19 publications

Overexpression of an alternative oxidase gene, OsAOX1a, improves cold tolerance in Oryza sativa L.

Overexpression of an alternative oxidase gene, OsAOX1a, improves cold tolerance in Oryza sativa L.

New insights into the genetic basis of natural chilling and cold shock tolerance in rice by genome‐wide association analysis

Physiological networks governing salinity tolerance potentials inGossypium hirsutumgermplasm

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