Phenotypic plasticity to chronic cold exposure in two species of Peromyscus from different environments

Hayward, Leah; Robertson, Cayleih E.; McClelland, Grant B.

doi:10.1007/s00360-021-01423-4

Cited by 7 publications

(4 citation statements)

References 56 publications

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“…1C ), associated with the increase in V̇ O 2 ,max ; a response not shared by lowland deer mice. This increase in lipid oxidation is consistent with the response observed when highlanders are acclimated to cold alone ( Hayward et al, 2022 ). In lowland deer mice, the addition of hypoxia may antagonize any cold-induced increases in whole-animal lipid oxidation, similarly to the response observed for NST in lab mice ( Beaudry and McClelland, 2010 ).…”

Section: Discussionsupporting

confidence: 88%

“…1A ). We used a TN acclimation (30°C, 21 kPa O 2 ) to ensure mice were housed within their thermoneutral zone ( Hayward et al, 2022 ). When both populations were acclimated to TN conditions, post hoc testing revealed no significant differences in cold-induced V̇ O 2 ,max .…”

Section: Discussionmentioning

confidence: 99%

“…Altogether, our findings suggest that highland deer mice acclimated to simulated high-altitude conditions increase maximal heat production with a corresponding increase in lipid metabolism, when compared with lowlanders, consistent with previous studies ( Tate et al, 2020 ; Lyons et al, 2021 ). These findings are associated with the increased cardiac output ( Tate et al, 2017 , 2020 ), circulatory fat delivery rates ( Lyons and McClelland, 2022 ), capillarity and oxidative phenotype of muscle ( Lui et al, 2015 ; Lau et al, 2017 ; Mahalingam et al, 2020 ; Lyons and McClelland, 2022 ), and aerobic capacity of iBAT ( Coulson et al, 2021 ; Hayward et al, 2022 ) of highland deer mice. While there has been emerging evidence for the increased role of circulatory lipids for powering thermogenesis in thermo-effector tissue ( Lyons and McClelland, 2022 ), the specific distribution of these circulatory lipids had yet to be determined in detail.…”

Section: Discussionmentioning

confidence: 99%

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Highland deer mice support increased thermogenesis in response to chronic cold hypoxia by shifting uptake of circulating fatty acids from muscles to brown adipose tissue

Lyons,

McClelland

2024

Journal of Experimental Biology

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During maximal cold challenge (cold-induced V˙O2max) in hypoxia, highland deer mice (Peromyscus maniculatus) show higher rates of circulatory fatty acid delivery compared with lowland deer mice. Fatty acid delivery also increases with acclimation to cold hypoxia (CH) and likely plays a major role in supporting the high rates of thermogenesis observed in highland deer mice. However, it is unknown which tissues take up these fatty acids and their relative contribution to thermogenesis. The goal of this study was to determine the uptake of circulating fatty acids into 24 different tissues during hypoxic cold-induced V˙O2max, by using [1-14C]2-bromopalmitic acid. To uncover evolved and environment-induced changes in fatty acid uptake, we compared lab born and raised highland and lowland deer mice, acclimated to either thermoneutral (TN; 30°C, 21 kPa O2) or CH (5°C, 12 kPa O2) conditions. During hypoxic cold-induced V˙O2max, CH acclimated highlanders decreased muscle fatty acid uptake and increased uptake into brown adipose tissue (BAT) relative to TN highlanders, a response was absent in lowlanders. CH acclimation was also associated with increased activities of enzymes citrate synthase and β-hydroxyacyl-CoA dehydrogenase in the BAT of highlanders, and higher FAT/CD36 abundance in both populations. This is the first study to show that cold-induced fatty acid uptake is distributed across a wide range of tissues. Highland deer mice show plasticity in this fatty acid distribution in response to CH, and combined with higher rates of tissue delivery, contributes to their survival in the cold high alpine.

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Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Highland deer mice support increased thermogenesis in response to chronic cold hypoxia by shifting uptake of circulating fatty acids from muscles to brown adipose tissue

Lyons,

McClelland

2024

Journal of Experimental Biology

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“…Protein content of uncoupling protein 1 (UCP1) and citrate synthase (CS) were measured in iBAT by western blotting as previously described (Hayward et al, 2022;Robertson et al, 2019). Tissues were first powdered using a liquid N 2 -cooled mortar and pestle, and ∼20 mg of powdered tissue was homogenized with a PowerGen 125 homogenizer (Thermo Fisher Scientific, Mississauga, ON, Canada) in cold RIPA buffer containing 150 mmol l −1 NaCl, 50 mmol l −1 Tris, 1% Triton X-100, 0.5% deoxycholic acid, 0.l% SDS and 1 tablet/10 ml of cOmplete™ Mini Protease Inhibitor Cocktail (Sigma-Aldrich) at pH 8.0.…”

Section: Western Blottingmentioning

confidence: 99%

Thermoregulatory trade-offs underlie the effects of warming summer temperatures on deer mice

Flewwelling

Wearing

Garrett

et al. 2023

Journal of Experimental Biology

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Climate warming could challenge the ability of endotherms to thermoregulate and maintain normal body temperature (Tb), but the effects of warming summer temperatures on activity and thermoregulatory physiology in many small mammals remain poorly understood. We examined this issue in deer mice (Peromyscus maniculatus), an active nocturnal species. Mice were exposed in the lab to simulated seasonal warming, in which an environmentally realistic diel cycle of ambient temperature (Ta) was gradually warmed from spring conditions to summer conditions (controls were maintained in spring conditions). Activity (voluntary wheel running) and Tb (implanted bio-loggers) were measured throughout, and indices of thermoregulatory physiology (thermoneutral zone, thermogenic capacity) were assessed after exposure. In control mice, activity was almost entirely restricted to the nighttime, and Tb fluctuated ∼1.7°C between daytime lows and nighttime highs. Activity, body mass and food consumption were reduced and water consumption was increased in later stages of summer warming. This was accompanied by strong Tb dysregulation that culminated in a complete reversal of the diel pattern of Tb variation, with Tb reaching extreme highs (∼40°C) during daytime heat but extreme lows (∼34°C) at cooler nighttime temperatures. Summer warming was also associated with reduced ability to generate body heat, as reflected by decreased thermogenic capacity and decreased mass and uncoupling protein (UCP1) content of brown adipose tissue. Our findings suggest that thermoregulatory trade-offs associated with daytime heat exposure can affect Tb and activity at cooler nighttime temperatures, impacting the ability of nocturnal mammals to perform behaviours important for fitness in the wild.

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Body size influences the capacity to cope with extreme cold or hot temperatures in the striped hamster

Hu,

Zhang,

Zhang

et al. 2024

Journal of Thermal Biology

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Phenotypic plasticity to chronic cold exposure in two species of Peromyscus from different environments

Cited by 7 publications

References 56 publications

Highland deer mice support increased thermogenesis in response to chronic cold hypoxia by shifting uptake of circulating fatty acids from muscles to brown adipose tissue

Highland deer mice support increased thermogenesis in response to chronic cold hypoxia by shifting uptake of circulating fatty acids from muscles to brown adipose tissue

Thermoregulatory trade-offs underlie the effects of warming summer temperatures on deer mice

Body size influences the capacity to cope with extreme cold or hot temperatures in the striped hamster

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