Phenotypic Screens Identify Genetic Factors Associated with Gametocyte Development in the Human Malaria Parasite Plasmodium falciparum

Chawla, Jyotsna; Goldowitz, Ilana; Oberstaller, Jenna; Zhang, Min; Pires, Camilla Valente; Navarro, Francesca M.; Sollelis, Lauriane; Wang, Chengqi; Seyfang, Andreas; Dvorin, Jeffrey D.; Otto, Thomas D.; Rayner, Julian C.; Marti, Matthias; Adams, John

doi:10.1128/spectrum.04164-22

Cited by 3 publications

(1 citation statement)

References 87 publications

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“…Several environmental and metabolic factors that trigger sexual commitment, including antimalarial drugs [ 97 , 98 ], lysophosphatidylcholine [ 99 , 100 ], host immune response [ 101 , 102 ], and parasite factors [ 103 , 104 ], have been reported. ApiAP2-G was the first transcription factor shown to govern the transcription of gametocyte-associated genes [ 24 , 25 ], and its regulation requires interaction with a second transcription factor, Pf AP2-I [ 105 ].…”

Section: Gametocyte Developmentmentioning

confidence: 99%

Plasmodium falciparum Development from Gametocyte to Oocyst: Insight from Functional Studies

Ouologuem,

Dara,

Kone

et al. 2023

Microorganisms

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Malaria elimination may never succeed without the implementation of transmission-blocking strategies. The transmission of Plasmodium spp. parasites from the human host to the mosquito vector depends on circulating gametocytes in the peripheral blood of the vertebrate host. Once ingested by the mosquito during blood meals, these sexual forms undergo a series of radical morphological and metabolic changes to survive and progress from the gut to the salivary glands, where they will be waiting to be injected into the vertebrate host. The design of effective transmission-blocking strategies requires a thorough understanding of all the mechanisms that drive the development of gametocytes, gametes, sexual reproduction, and subsequent differentiation within the mosquito. The drastic changes in Plasmodium falciparum shape and function throughout its life cycle rely on the tight regulation of stage-specific gene expression. This review outlines the mechanisms involved in Plasmodium falciparum sexual stage development in both the human and mosquito vector, and zygote to oocyst differentiation. Functional studies unravel mechanisms employed by P. falciparum to orchestrate the expression of stage-specific functional products required to succeed in its complex life cycle, thus providing us with potential targets for developing new therapeutics. These mechanisms are based on studies conducted with various Plasmodium species, including predominantly P. falciparum and the rodent malaria parasites P. berghei. However, the great potential of epigenetics, genomics, transcriptomics, proteomics, and functional genetic studies to improve the understanding of malaria as a disease remains partly untapped because of limitations in studies using human malaria parasites and field isolates.

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Section: Gametocyte Developmentmentioning

confidence: 99%

Plasmodium falciparum Development from Gametocyte to Oocyst: Insight from Functional Studies

Ouologuem,

Dara,

Kone

et al. 2023

Microorganisms

View full text Add to dashboard Cite

show abstract

Peeling the onion: how complex is the artemisinin resistance genetic trait of malaria parasites?

Kucharski,

Nayak,

Gendrot

et al. 2024

Trends in Parasitology

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Population genomics and transcriptomics of Plasmodium falciparum in Cambodia and Vietnam uncover key components of the artemisinin resistance genetic background

Nayak,

Peto,

Kucharski

et al. 2024

Nat Commun

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Phenotypic Screens Identify Genetic Factors Associated with Gametocyte Development in the Human Malaria Parasite Plasmodium falciparum

Abstract: Blocking human-to-vector transmission is an essential step toward malaria elimination. Gametocytes are solely responsible for achieving this transmission and represent an opportunity for therapeutic intervention.

Cited by 3 publications

References 87 publications

Plasmodium falciparum Development from Gametocyte to Oocyst: Insight from Functional Studies

Plasmodium falciparum Development from Gametocyte to Oocyst: Insight from Functional Studies

Peeling the onion: how complex is the artemisinin resistance genetic trait of malaria parasites?

Population genomics and transcriptomics of Plasmodium falciparum in Cambodia and Vietnam uncover key components of the artemisinin resistance genetic background

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