Phenotypic, Stress Tolerance and Plant Growth Promoting Characteristics of Rhizobial Isolates from Selected Wild Legumes of Semiarid Region, Tirupati, India

Bhargava, Yogesh; Murthy, J. S. R.; Kumar, T. Vinoth; Rao, M. Narayana

doi:10.4236/aim.2016.61001

Cited by 21 publications

(8 citation statements)

References 26 publications

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“…Our results concerning the effect of inoculation by the tested bacteria on Acacia cyanophylla seed germination are consistent with several studies [26,41,44] which claimed that the use of PGPR specifically IAA producing bacteria might have a significant effect on increasing seed germination rate and promoting plant growth.…”

Section: Discussionsupporting

confidence: 92%

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Screening and optimization of indole-3-acetic acid production by Rhizobium sp. strain using response surface methodology

Lebrazi

Fadil

Chraibi

et al. 2020

Journal of Genetic Engineering and Biotechnology

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Background: The production of indole-3-acetic acid (IAA) is an essential tool for rhizobacteria to stimulate and facilitate plant growth. For this, eighty rhizobial bacteria isolated from root nodules of Acacia cyanophylla grown in different regions of Morocco were firstly screened for their ability to produce IAA. Then, IAA production by a combination of isolates and the inoculation effect on the germination of Acacia cyanophylla seeds was studied using the best performing isolates in terms of IAA production. The best IAA producer bacterial isolate (I69) was selected to optimize IAA production using response surface methodology based on the central composite design. Results: Results showed that the majority of tested isolates were able to produce IAA with a relatively higher concentration of 135 μg/ml for the isolate I69, followed by isolates I22 and I75 with respective concentrations of 116 μg/ml and 105 μg/ml IAA. The IAA production and the seed germination rate were relatively increased by the synergistic effect of I69 and I22. Later, response surface methodology was used to determine optimal operating conditions leading to IAA production optimization. Thus, an incubation temperature of 36°C, a pH of 6.5, an incubation time of 1 day, and respective tryptophan and NaCl concentrations of 1 g/l and 0.1 g/l were optimal parameters leading to 166 μg/ml IAA which was the maximal produced concentration. Conclusion: The present study highlighted that IAA-producing rhizobacteria could be harnessed to improve plant growth. Furthermore, their production can be easily controlled using response surface methodology, which represents a very useful tool for optimization.

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Section: Discussionsupporting

confidence: 92%

“…The variation in IAA production by different rhizobial isolates and the influence of cultural conditions on IAA produced amount has been reported by many authors [39][40][41]. The variation in IAA production is may be due to variation in L-Trp utilization by each isolate.…”

Section: Discussionmentioning

confidence: 93%

Screening and optimization of indole-3-acetic acid production by Rhizobium sp. strain using response surface methodology

Lebrazi

Fadil

Chraibi

et al. 2020

Journal of Genetic Engineering and Biotechnology

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“…They were purified and propagated using YMA medium (Yeast Mannitol Agar). The inoculants were propagated using YMA medium without agar (Bhargava et al, 2016).…”

Section: Methodsmentioning

confidence: 99%

Responses of Phaseolus calcaltus to lime and biochar application in an acid soil

Yao

Huang

et al. 2019

PeerJ

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IntroductionRice bean (Phaseolus calcaltus), as an annual summer legume, is always subjected to acid soils in tropical to subtropical regions, limiting its growth and nodulation. However, little is known about its responses to lime and biochar addition, the two in improving soil fertility in acid soils.Materials and MethodsIn the current study, a pot experiment was conducted using rice bean on a sandy yellow soil (Orthic Acrisol) with a pH of 5.5. The experiment included three lime rates (0, 0.75 and 1.5 g kg−1) and three biochar rates (0, 5 and 10 g kg−1). The biochar was produced from aboveground parts of Solanum tuberosum using a home-made device with temperature of pyrolysis about 500 °C.Results and DiscussionThe results indicated that both lime and biochar could reduce soil exchange Al concentration, increase soil pH and the contents of soil microbial biomass carbon and microbial biomass nitrogen, and enhance urease and dehydrogenase activities, benefiting P. calcaltus growth and nodulation in acid soils. Lime application did decrease the concentrations of soil available phosphorus (AP) and alkali dispelled nitrogen (AN), whereas biochar application increased the concentrations of soil AP, AN and available potassium (AK). However, sole biochar application could not achieve as much yield increase as lime application did. High lime rate (1.5 g lime kg−1) incorporated with low biochar rate (5 g biochar kg−1) could obtain higher shoot biomass, nutrient uptake, and nodule number when compared with high lime rate and high biochar rate.ConclusionLime incorporated with biochar application could achieve optimum improvement for P. calcaltus growing in acid soils when compared with sole lime or biochar addition.

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“…Florentino et al (2010) also reported that rhizobia isolated from cowpea were able to tolerate temperatures of 28°C and 36°C, and none of the strains grew at 45°C. The higher temperature is reported to alter the permeability of the membrane and leading to the denaturation of enzymes/ proteins causing the death and/or poor growth of the rhizobia (Bhargava et al, 2016).…”

Section: Temperature Tolerancementioning

confidence: 99%

Eco-physiological and physiological characterization of cowpea nodulating native rhizobia isolated from major production areas of Ethiopia

et al. 2020

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Phenotypic, Stress Tolerance and Plant Growth Promoting Characteristics of Rhizobial Isolates from Selected Wild Legumes of Semiarid Region, Tirupati, India

Cited by 21 publications

References 26 publications

Screening and optimization of indole-3-acetic acid production by Rhizobium sp. strain using response surface methodology

Screening and optimization of indole-3-acetic acid production by Rhizobium sp. strain using response surface methodology

Responses of Phaseolus calcaltus to lime and biochar application in an acid soil

Eco-physiological and physiological characterization of cowpea nodulating native rhizobia isolated from major production areas of Ethiopia

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