Phenotypical changes of hematopoietic stem
and progenitor cells in COVID-19 patients:
Correlation with disease status

Hussein, Hosni A. M.; Thabet, Ali A.; Taha, Mohamed; Elnosary, Mohamed E.; Sobhy, Ali; El-Adly, Ahmed M.; Wardany, Ahmed A.; Bakhiet, Elsayed K.; Afifi, Magdy; Abdul‐Raouf, Usama M.; Fathy, Samah M.; Sayed, Noha G; Zahran, Asmaa M

doi:10.5114/ceji.2023.129981

Cited by 8 publications

(7 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recent research ( Avsar et al, 2016 ) proved that this oil treated second-degree burns in rats more quickly than silver sulfadiazine, exhibiting strong TGF-1 expression and wound closure. Additionally, it has a high concentration of monounsaturated and polyunsaturated fatty acids (with an average value of more than 80%), including oleic acid (50%) and linoleic acid (30%), which have antibacterial properties ( Gharby and Charrouf, 2022 ; Almuhayawi et al, 2023b ; Hussein et al, 2023 ), and argan oil can shield the wound from the outside environment. However, in the present trial, no wounds developed an infection.…”

Section: Discussionmentioning

confidence: 99%

In vitro and in silico biopotentials of phytochemical compositions and antistaphylococcal and antipseudomonal activities of volatile compounds of Argania spinosa (L.) seed oil

Almuhayawi,

Alruhaili,

Gattan

et al. 2024

Front. Bioeng. Biotechnol.

Self Cite

View full text Add to dashboard Cite

Active components in medicinal plants provide unlimited useful and traditional medicines. Antimicrobial activities are found in secondary metabolites in plant extracts such as argan oil. This experimental investigation aims to determine argan oil’s volatile compounds and examine their in vitro antimicrobial properties. In silico simulations, molecular docking, pharmacokinetics, and drug-likeness prediction revealed the processes underlying the in vitro biological possessions. Gas chromatography–mass spectrometry (GC/MS) was used to screen argan oil’s primary components. In silico molecular docking studies were used to investigate the ability of the selected bioactive constituents of argan oil to act effectively against Pseudomonas aeruginosa and Staphylococcus aureus (S. aureus) isolated from infections. The goal was to study their ability to interact with both bacteria’s essential therapeutic target protein. The 21 chemicals in argan oil were identified by GC/MS. Docking results for all compounds with S. aureus and P. aeruginosa protease proteins ranged from −5 to −9.4 kcal/mol and −5.7 to −9.7 kcal/mol, respectively, compared to reference ligands. Our docking result indicates that the 10-octadecenoic acid, methyl ester was the most significant compound with affinity scores of −9.4 and −9.7 kcal/mol for S. aureus and P. aeruginosa proteins, respectively. The minimal bactericidal concentration (MBC) and minimal inhibitory concentration (MIC) of argan oil were 0.7 ± 0.03 and 0.5 ± 0.01 for S. aureus and 0.4 ± 0.01 and 0.3 ± 0.02 for P. aeruginosa, respectively. We confirmed the antimicrobial properties of argan oil that showed significant growth inhibition for S. aureus and P. aeruginosa.

show abstract

Section: Discussionmentioning

confidence: 99%

In vitro and in silico biopotentials of phytochemical compositions and antistaphylococcal and antipseudomonal activities of volatile compounds of Argania spinosa (L.) seed oil

Almuhayawi,

Alruhaili,

Gattan

et al. 2024

Front. Bioeng. Biotechnol.

Self Cite

View full text Add to dashboard Cite

show abstract

“…In addition, molecular docking of the biosurfactant’s active components showed antiviral behavior against the zika virus and the human immunodeficiency virus. Like Zika, dengue virus (DENV) is a flavivirus ( Culshaw et al, 2017 ; Shu et al, 2021 ; Elshazly et al, 2023 ; Hussein et al, 2023 ; Selim et al, 2023 ) that can cause serious illness or death. The use of broad-spectrum antiviral inhibitors might be extremely helpful in the treatment of ZIK virus and other flavivirus infections.…”

Section: Discussionmentioning

confidence: 99%

Exploring biosurfactant from Halobacterium jilantaiense as drug against HIV and zika virus: fabrication, characterization, cytosafety property, molecular docking, and molecular dynamics simulation

Almuhayawi,

Elshafey,

Hagagy

et al. 2024

Front. Bioeng. Biotechnol.

Self Cite

View full text Add to dashboard Cite

Biosurfactants are surface-active molecules with unique qualities and various uses. Many microorganisms produce secondary metabolites with surface-active characteristics that serve various antiviral functions. The HIV and Zika viruses were chosen for this study because they can spread from mother to child and result in potentially fatal infections in infants. Halophilic bacteria from the Red Sea solar saltern in Egypt were screened using drop collapse, emulsification activity, and oil displacement assays to produce biosurfactants and emulsifiers. Halobacterium jilantaiense strain JBS1 was the most effective strain of the Halobacteriaceae family. It had the best oil displacement test and emulsification activity against kerosene and crude oil, respectively. Among the ten isolates, it produced the most promising biosurfactant, also recognized by the GC-MASS library. This study evaluated biosurfactants from halophilic bacteria as potential antiviral drugs. Some of the computer methods we use are molecular docking, ADMET, and molecular dynamics. We use model organisms like the HIV reverse transcriptase (PDB: 5VZ6) and the Zika virus RNA-dependent RNA polymerase (ZV-RdRP). Molecular docking and molecular dynamics make the best complexes with 5VZ6 HIV-RT and flavone (C25) and 5wz3 ZV-RdRP and ethyl cholate (C8). Testing for ADMET toxicity on the complex revealed that it is the safest medicine conceivable. The 5VZ6-C25 and 5wz3-C8 complexes also followed the Lipinski rule. They made five hydrogen bond donors and ten hydrogen bond acceptors with 500 Da MW and a 5:1 octanol/water partition coefficient. Finally, extreme settings require particular adaptations for stability, and extremophile biosurfactants may be more stable.

show abstract

“…Morgan and Bodsky in 2004 described these immature HERV-K particles which budded into vacuoles in megakaryocytes in patients with essential thrombocytopenia [125]. An unknown virus appeared to be budding from the cell surface of the megakaryocytes suggesting a viral infection may have led to the induction of HERV-K immature particles putatively in the Common Myeloid Progenitor (CMP) that macrophages and megakaryocytes share [12]. Presumably, the release of the particles from the megakaryocytes would have also been through cell apoptosis, and this lysis and loss of megakaryocytes presumably would have resulted in thrombocytopenia.…”

Section: Evidence For a Role Of Herv-k Hml-2 Activation In Innate Imm...mentioning

confidence: 99%

“…Dendritic cells that are the antigen presenters for adaptive immune responses are derived from a separate set of bone marrow progenitors, called the Common Lymphoid Progenitors (CLPs). Instead, macrophages (and monocytes) are derived from the Common Myeloid Progenitors (CMPs) which then generate the Granulocyte-Macrophage Progenitors (GMPs) and the Megakaryocyte-Erythroid Progenitors (MEPs) [12]. Accordingly, discussions of monocytes in the blood or macrophages in the tissues refer and pertain to innate immunity.…”

mentioning

confidence: 99%

Antibody Dependent Enhancement (ADE) of Infection into Macrophages Validates the Importance of HERV-K102 Particle Production for Pandemic Preparedness

Laderoute

2023

Preprint

View full text Add to dashboard Cite

Historically, macrophages have been long implicated in the control of the severity of infectious diseases. This has been based on the observations of a higher risk of severe disease and death upon rechallenge with viral variants due to antibody dependent enhance-ment (ADE) of infection into macrophages. The question remains as to what can account for this potent heterologous protection in macrophages? Here it is argued that the elusive defense mechanism of M1-like pro-inflammatory macrophages may pertain to a novel virus anti-virus response. This system initiates with high replication of human endogenous retrovirus K102 (HERV-K102), a non-pathogenic, protector foamy retrovirus of humans which generates M1-like pro-inflammatory foamy macrophages, glycolysis, and epigenetic changes, all characteristic of trained immunity. This virus-anti-virus system kills virally infected cells by several mechanisms, amplifies the innate interferon response via ‘viral mimicry’, has many unique components that interfere with exoge-nous virus replication, and may be especially adept at neutralizing enveloped exogenous pandemic viruses, such as SARS-CoV-2 and HIV-1. The goal of this treatise is to introduce the multifaceted HERV-K102 protector system, to illustrate how SARS-CoV-2 may target the HERV-K102 protector system by ADE, and to explore how this innate defense system may be exploited for pandemic preparedness.

show abstract

Phenotypical changes of hematopoietic stem and progenitor cells in COVID-19 patients: Correlation with disease status

Cited by 8 publications

References 29 publications

In vitro and in silico biopotentials of phytochemical compositions and antistaphylococcal and antipseudomonal activities of volatile compounds of Argania spinosa (L.) seed oil

In vitro and in silico biopotentials of phytochemical compositions and antistaphylococcal and antipseudomonal activities of volatile compounds of Argania spinosa (L.) seed oil

Exploring biosurfactant from Halobacterium jilantaiense as drug against HIV and zika virus: fabrication, characterization, cytosafety property, molecular docking, and molecular dynamics simulation

Antibody Dependent Enhancement (ADE) of Infection into Macrophages Validates the Importance of HERV-K102 Particle Production for Pandemic Preparedness

Contact Info

Product

Resources

About