Phenthoate toxicity evaluation in root meristem of Pisum sativum L.

Siddiqui, Sazada

doi:10.36253/caryologia-2036

Cited by 2 publications

(1 citation statement)

References 52 publications

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“…The fragments formed from chromatid and chromosomal break imply its mutagenic events within the cell. In a previous study, Siddiqui et al (2020 a,b) had reported that pesticides cause various chromosomal anomalies. Generation of giant cells having diverse chromosomal anomalies had been reported in a previous study by food colorants (Prajitha and Thoppil 2016).…”

Section: Discussionmentioning

confidence: 99%

Methomyl has clastogenic and aneugenic effects and alters the mitotic kinetics in Pisum sativum L.

Siddiqui

Alrumman

2023

Caryologia

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Methomyl is a carbamate pesticide that is frequently applied to crops all over the world. This research aims to evaluate the Pisum sativum L mitotic process and potential genotoxicity. The Cell Proliferation Kinetics (CPK) frequencies demonstrated changes in kinetics of mitotic process, and study of Mitotic Index (MI) demonstrated that methomyl had cytotoxic properties. In fact, the telophases ratio dropped at 0.1% to 0.5% methomyl treatment, while there was an increase in prophases, metaphases, and anaphases from 0.1% to 0.5% in a dose dependent manner. In terms of genotoxicity, methomyl cause an increase in the frequency of clastogenic and aneugenic chromosomal abnormalities at metaphase-anaphase at 0.1% to 0.5%. The effects on the mitotic spindle were further confirmed by an increase in the frequencies of c-mitosis from 0.1 to 0.5% methomyl treatment. The outcome of the current analysis indicates that regularly used insecticides methomyl has a considerable cytotoxic effect on mitotic cells of Pisum sativum L.

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Section: Discussionmentioning

confidence: 99%

Methomyl has clastogenic and aneugenic effects and alters the mitotic kinetics in Pisum sativum L.

Siddiqui

Alrumman

2023

Caryologia

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DNA Damage, Cell Death, and Alteration of Cell Proliferation Insights Caused by Copper Oxide Nanoparticles Using a Plant-Based Model

Siddiqui

2024

Biology

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The speedy growth of copper oxide nanoparticle (CuO NP) manufacturing due to their wide application in industries has caused concerns due to their increased discharge into the environment from both purposeful and accidental sources. Their presence at an elevated concentration in the environment can cause potential hazards to the plant kingdom, specifically to staple food crops. However, limited research is available to determine the consequences of CuO NPs. The present study aimed to assess the morphological and cytological changes induced by CuO NPs on Pisum sativum L., a key staple food crop. Seeds of Pisum sativum were exposed to various concentrations of CuO NPs (0, 25, 50, 75, 100, and 125 ppm) for 2 h, and their effects on seed germination (SG), radicle length (RL), cell proliferation kinetics (CPK), mitotic index (MI), cell death (CD), micronucleus frequency (MNF), and chromosomal aberration frequency (CAF) were studied. The results indicate a significant reduction in SG, RL, CPK, and MI and a significant dose-dependent increase in CD, MNF, and CAF. CuO NP treatment has led to abnormal meiotic cell division, increased incidence of micronucleus frequency, and chromosomal aberration frequency. Additionally, the CuO NP-treated groups showed an increase in the percentage of aberrant meiotic cells such as laggard (LG), double bridge (DB), stickiness (STC), clumped nuclei (CNi), precocious separation (PS), single bridge (SB), and secondary association (SA). CuO NP treatment led to reductions in SG as follows: 55% at 24 h, 60.10% at 48 h, and 65% at 72 h; reductions in RL as follows: 0.55 ± 0.021 cm at 24 h, 0.67 ± 0.01 cm at 48 h, and 0.99 ± 0.02 cm at 72 h; reductions in CPK as follows: 34.98% at prophase, 7.90% at metaphase, 3.5% at anaphase, and 0.97% at telophase. It also led to a 57.45% increase in CD, a 39.87% reduction in MI, and a 60.77% increase in MNF at a higher concentration of 125 ppm. The findings of this study clearly show that CuO NPs have a genotoxic effect on the food crop plant Pisum sativum.

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Phenthoate toxicity evaluation in root meristem of Pisum sativum L.

Cited by 2 publications

References 52 publications

Methomyl has clastogenic and aneugenic effects and alters the mitotic kinetics in Pisum sativum L.

Methomyl has clastogenic and aneugenic effects and alters the mitotic kinetics in Pisum sativum L.

DNA Damage, Cell Death, and Alteration of Cell Proliferation Insights Caused by Copper Oxide Nanoparticles Using a Plant-Based Model

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