Phenylethylamides derived from bacterial secondary metabolites specifically inhibit an insect serotonin receptor

Hasan, Ariful; Yeom, Hankil; Ryu, Jae-Wook; Bode, Helge B.; Kim, Yonggyun

doi:10.1038/s41598-019-56892-z

Cited by 14 publications

(10 citation statements)

References 69 publications

(76 reference statements)

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“…Most genes showed significantly high expression in the hemolymph tissues (Fig. 5), suggesting that they may be important molecules bridging the immune system 94–99 . From all Px5‐HTRs, Px5‐HT 7 was the most abundant in midgut tissues, consistent with previous studies in Pieris rapae 100 and indicating the crucial role of Px5‐HT 7 in the gut.…”

Section: Discussionsupporting

confidence: 89%

Molecular and pharmacological characterization of biogenic amine receptors from the diamondback moth, Plutella xylostella

Liu

Zhan

et al. 2021

Pest Management Science

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BACKGROUD Insect biogenic amines play important roles in mediating behavioral and physiological processes. They exert their effects by binding to biogenic amine receptors (BARs), which are specific receptor proteins in the G‐protein‐coupled receptor superfamily. BAR genes have been cloned and characterized from multiple model insects, including Drosophila melanogaster, Anopheles gambiae, Bombyx mori, Apis mellifera and Tribolium castaneum. However, relatively little work has addressed the molecular properties, expression profiles, and pharmacological characterization of BARs from other insects, including important pests. RESULTS In this study, we cloned 17 genes encoding putative biogenic amine receptor proteins from Plutella xylostella, a global pest of Brassica crops. These PxBAR genes were five octopamine receptors (PxOA1, PxOA2B1, PxOA2B2, PxOA2B3, and PxOA3), three tyramine receptors (PxTAR1A, PxTAR1B, and PxTAR2), four dopamine receptors (PxDOP1, PxDOP2, PxDOP3, and PxDopEcR), and five serotonin receptors (Px5‐HT1A, Px5‐HT1B, Px5‐HT2A, Px5‐HT2B, and Px5‐HT7). All PxBARs showed considerable sequence identity with orthologous BARs, and phylogenetic analysis clustered the receptors within their respective groups while preserving organismal evolutionary relationships. We investigated their molecular properties and expression profiles, and pharmacologically characterized the dopamine receptor, PxDOP2. CONCLUSIONS Our study provides important information and resources on biogenic amine receptors from P. xylostella, which suggests potential target sites for controlling this pest species. © 2021 Society of Chemical Industry.

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Section: Discussionsupporting

confidence: 89%

Molecular and pharmacological characterization of biogenic amine receptors from the diamondback moth, Plutella xylostella

Liu

Zhan

et al. 2021

Pest Management Science

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“…In contrast, honey samples without antimicrobial activity (Group 1) were characterized by the presence of several phenylethylamides. According to the literature, these substances are secondary metabolites produced by entomopathogenic bacteria and can interact with insect serotonin receptors generating significant immunosuppressive effects . Phenylethylamides can also induce immunosuppression by inhibiting eicosanoid biosynthesis leading to fatal septicemia in insects .…”

Section: Discussionmentioning

confidence: 99%

“…According to the literature, these substances are secondary metabolites produced by entomopathogenic bacteria and can interact with insect serotonin receptors generating significant immunosuppressive effects. 46 Phenylethylamides can also induce immunosuppression by inhibiting eicosanoid biosynthesis leading to fatal septicemia in insects. 47 From the point of view of stingless beekeeping (meliponiculture), these findings need to be cautiously investigated in the future because they may indicate some unidentified pathology in the native stingless beehives and the potential effects of phenylethylamides on human health are unknown.…”

Section: ■ Discussionmentioning

confidence: 99%

Flavonoids and Phenylethylamides Are Pivotal Factors Affecting the Antimicrobial Properties of Stingless Bee Honey

Dallagnol

Vignolo

et al. 2022

J. Agric. Food Chem.

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Despite the recent approval of stingless bee honey to the Argentine Food Code, there are still many gaps in information. Likely, the main reason for this is that multiple ecological and chemical factors influence their production and antimicrobial properties. This work combined metabolomic, microbiological, and physicochemical analyses to characterize the honey ofTetragonisca fiebrigifrom Northeastern Argentina. The antimicrobial activity tests showed that honey samples (n = 24) inhibited some Gram-positive and Gram-negative bacteria at different sensitivity levels. Furthermore, samples selected for their high bioactivity revealed crystallizations, a positive correlation with fungal growth, and the presence of flavonoids. The major polyphenols annotated by liquid chromatography with tandem mass spectrometry (LC-MS/MS) analysis and supported by metabolomic tools were quercetin 3,4′-dimethyl ether, pachypodol, jaceoside, irigenin trimethyl ether, corymboside, chrysoeriol 7-neohesperidoside, and corymboside. In contrast, samples missing antimicrobial activity did not crystallize, lacked flavonoids, and were enriched in phenylethylamides. Based on these findings, we discuss the significance of flavonoids and phenylethylamides on honey’s antimicrobial activity and food quality and how they may indeed reflect essential parameters of the hive, such as microbial balance and eubiosis.

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“…Aliphatic nitrilase with b-ureidopropionase activity is involved in the catabolism of pyrimidine and the production of b-alanine (36) that has a role in cuticular tanning and sclerotization (37). The relatively high levels of 5-hydroxytryptamine (serotonin) receptor may reflect the regulation of phagocytosis by serotonin, as is observed in Drosophila (38,39). Interestingly, another gene with high log2FC ranking is characterized with a LPS-induced TNF-activating factor (LITAF) domain that mediates targeting to intracellular membranes to regulate endosomal trafficking (40).…”

Section: Cluster 0 ("Granulocyte")mentioning

confidence: 99%

Hemocyte Clusters Defined by scRNA-Seq in Bombyx mori: In Silico Analysis of Predicted Marker Genes and Implications for Potential Functional Roles

2022

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Within the hemolymph, insect hemocytes constitute a heterogeneous population of macrophage-like cells that play important roles in innate immunity, homeostasis and development. Classification of hemocytes in different subtypes by size, morphology and biochemical or immunological markers has been difficult and only in Drosophila extensive genetic analysis allowed the construction of a coherent picture of hemocyte differentiation from pro-hemocytes to granulocytes, crystal cells and plasmatocytes. However, the advent of high-throughput single cell technologies, such as single cell RNA sequencing (scRNA-seq), is bound to have a high impact on the study of hemocytes subtypes and their phenotypes in other insects for which a sophisticated genetic toolbox is not available. Instead of averaging gene expression across all cells as occurs in bulk-RNA-seq, scRNA-seq allows high-throughput and specific visualization of the differentiation status of individual cells. With scRNA-seq, interesting cell types can be identified in heterogeneous populations and direct analysis of rare cell types is possible. Next to its ability to profile the transcriptomes of individual cells in tissue samples, scRNA-seq can be used to propose marker genes that are characteristic of different hemocyte subtypes and predict their functions. In this perspective, the identities of the different marker genes that were identified by scRNA-seq analysis to define 13 distinct cell clusters of hemocytes in larvae of the silkworm, Bombyx mori, are discussed in detail. The analysis confirms the broad division of hemocytes in granulocytes, plasmatocytes, oenocytoids and perhaps spherulocytes but also reveals considerable complexity at the molecular level and highly specialized functions. In addition, predicted hemocyte marker genes in Bombyx generally show only limited convergence with the genes that are considered characteristic for hemocyte subtypes in Drosophila.

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Phenylethylamides derived from bacterial secondary metabolites specifically inhibit an insect serotonin receptor

Cited by 14 publications

References 69 publications

Molecular and pharmacological characterization of biogenic amine receptors from the diamondback moth, Plutella xylostella

Molecular and pharmacological characterization of biogenic amine receptors from the diamondback moth, Plutella xylostella

Flavonoids and Phenylethylamides Are Pivotal Factors Affecting the Antimicrobial Properties of Stingless Bee Honey

Hemocyte Clusters Defined by scRNA-Seq in Bombyx mori: In Silico Analysis of Predicted Marker Genes and Implications for Potential Functional Roles

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