Phenylpropanoid biosynthetic gene expression and nutrient uptake kinetics for enhanced rosmarinic acid production in suspension cultured cells of Halodule pinifolia

Danaraj, Jeyapragash; Mariasingarayan, Yosuva; Periakaruppan, Rajiv; Krishna, Jayachandran; Raja, Madhan; Saravanakumar, A.; Sivaramakrishnan, Ramachandran; Incharoensakdi, Aran

doi:10.1016/j.algal.2022.102675

Cited by 3 publications

(1 citation statement)

References 55 publications

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“…The TAT activity was determined according to the previous method by Danaraj et al (2022) with slight modifications. The TAT was extracted with 0.1 M phosphate buffer (pH 7.3) containing 0.1 M EDTA, 1 mM DTT, and 0.2 M VitB 6 .…”

Section: Determination Of Pal and Tat Enzyme Activitiesmentioning

confidence: 99%

Melatonin enhances biomass, phenolic accumulation, and bioactivities of rosemary (Rosmarinus officinalis) in vitro shoots under UV‐B stress

Zhao

Yun

et al. 2023

Physiologia Plantarum

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Melatonin is a stress‐related hormone that plays a critical role in triggering the plant defence system and regulating secondary metabolism when plants are exposed to stress. To explore the potential roles of melatonin in response to Ultraviolet‐B (UV‐B) radiation, we examined the effects of exogenous melatonin on rosemary in vitro shoots under UV‐B stress. The application of melatonin (50 μM) alleviated the adverse effects of UV‐B stress on the biomass, photosynthetic pigment contents, and membrane lipids of the rosemary in vitro shoots. Melatonin significantly increased superoxide dismutase (1.15.1.1, SOD), peroxidase (1.11.1.7, POD), and catalase (1.11.1.6, CAT) activities by 62%, 99%, and 53%, respectively. The contents of total phenols, rosmarinic acid, and carnosic acid increased under UV‐B stress, and they further increased by the melatonin treatment by 41%, 68%, and 67%, respectively, compared with the control group. Under UV‐B stress, the increased total phenol content in melatonin‐pretreated plants could be attributed to the activation of phenylalanine ammonia‐lyase (4.3.1.5, PAL) and tyrosine aminotransferase (2.6.1.5, TAT). In addition, melatonin enhanced the antioxidant and antibacterial activities of the rosemary in vitro shoots under UV‐B stress. These results suggest that melatonin can alleviate the damage caused by UV‐B stress and also enhance the secondary metabolism and bioactivity of rosemary in vitro shoots.

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Section: Determination Of Pal and Tat Enzyme Activitiesmentioning

confidence: 99%

Melatonin enhances biomass, phenolic accumulation, and bioactivities of rosemary (Rosmarinus officinalis) in vitro shoots under UV‐B stress

Zhao

Yun

et al. 2023

Physiologia Plantarum

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Melatonin alleviates UV‐B stress and enhances phenolic biosynthesis in rosemary (Rosmarinus officinalis) callus

Zhao,

Liu,

Yun

et al. 2024

Physiologia Plantarum

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Although used in in vitro culture to boost secondary metabolite production, UV‐B radiation can seriously affect plant growth if not properly dosed. Rosemary callus can be used as an important source of effective ingredients in the food and medicine industry. To balance the positive and negative effects of UV‐B on rosmary callus, this study investigated the effects of melatonin on rosemary callus under UV‐B radiation. The results showed that melatonin improved rosemary callus growth, with fresh weight and dry weight increased by 15.81% and 8.30%, respectively. The addition of 100 μM melatonin increased antioxidant enzyme activity and NO content in rosemary callus. At the same time, melatonin also significantly reduced membrane lipid damage and H2O2 accumulation in rosemary callus under UV‐B stress, with malondialdehyde (MDA) and H2O2 contents reduced by 13.03% and 14.55%, respectively. In addition, melatonin increased the total phenol and rosmarinic acid contents in rosemary callus by 19% and 54%, respectively. Melatonin significantly improved the antioxidant activity of the extracts from rosemary callus. These results suggest that exogenous melatonin can alleviate the adverse effects of UV‐B stress on rosemary callus by promoting NO accumulation while further enhancing phenolic accumulation and biological activity.

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Elicitor-Induced Metabolomics Analysis of Halodule pinifolia Suspension Culture for an Alternative Antifungal Screening Approach against Candida albicans

Alghuthaymi

Danaraj

Albarakaty

et al. 2022

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Elicitors are the agents that stimulate the defense responses of plants, and accumulate specialized metabolites in plant tissue culture. This study investigated the elicitor-feeding response of H. pinifolia suspension cell cultures (SCC) for metabolomics analysis and screening of specialized compounds against Candida albicans. Methyl jasmonate (MeJA) was used as an elicitor, and treatment of SCC at a concentration of 20 µM MeJA resulted in the maximum rosmarinic acid (RA) accumulation (117 mg/g dry weight), with transcript levels of RA biosynthetic genes HpPAL, HpC4H, and Hp4CL being 4.2, 2.5, and 3.7-fold higher, respectively, than the controls. GC-MS-based metabolomics analysis revealed a total of 47 metabolites, including 30 organic acids, six amino acids, six flavonoids, two sugars, two plant growth regulators, and one vitamin, which were significantly different between control and MeJA-treated cells. Furthermore, five phenolic acids were discovered at higher concentrations, including p-anisic acid, p-coumaric acid, caffeic acid, vanillic acid, and rosmarinic acid, and were purified and structurally elucidated for alternative antifungal screening against C. albicans and the evaluation of ADMET properties. The results from antifungal screening revealed that RA at MIC of 31.25 mg/L exhibited the lowest growth percentage of C. albicans (1.99%), with higher inhibition of isocitrate lyase 1 (ICL 1) enzyme (93.1%), followed by p-anisic acid (86.2%) and caffeic acid (85.1%), respectively. The drug likeliness and ADMET properties of RA exhibited promising results, with a bioactivity score of 0.57, 0.15, and 0.24 for nuclear receptor ligand, protease inhibitor, and enzyme inhibitor, respectively. Therefore, MeJA appears to have a significant effect on enhanced RA accumulation in H. pinifoia cells with phenylpropanoid transcript expression, and acts as an ICL1 inhibitor of C. albicans.

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Phenylpropanoid biosynthetic gene expression and nutrient uptake kinetics for enhanced rosmarinic acid production in suspension cultured cells of Halodule pinifolia

Cited by 3 publications

References 55 publications

Melatonin enhances biomass, phenolic accumulation, and bioactivities of rosemary (Rosmarinus officinalis) in vitro shoots under UV‐B stress

Melatonin enhances biomass, phenolic accumulation, and bioactivities of rosemary (Rosmarinus officinalis) in vitro shoots under UV‐B stress

Melatonin alleviates UV‐B stress and enhances phenolic biosynthesis in rosemary (Rosmarinus officinalis) callus

Elicitor-Induced Metabolomics Analysis of Halodule pinifolia Suspension Culture for an Alternative Antifungal Screening Approach against Candida albicans

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