Phenypressin (Phe2-Arg8-vasopressin), a new neurohypophysial peptide found in marsupials

Chauvet, M.T.; Hurpet, D.; Chauvet, Jacqueline; Acher, Roger

doi:10.1038/287640a0

Cited by 53 publications

(8 citation statements)

References 11 publications

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“…Thus, in most mammals, AVP has an antidiuretic effect on the distal kidney tubules and a pressor effect on vascular smooth muscle. However, in the pig and hippopotamus (and possibly other members of the order suina), these effects are mediated by lysine vasopressin (95,96) and not AVP; similarly, phenypressin plays this role in marsupials (97). OT causes the contraction of uterine smooth muscle in mammals; isotocin, mesotocin, and glumitocin, found in amphibia and fishes (98), demonstrate an OT-like activity.…”

Section: Other Naturally Occurring Nonapeptidesmentioning

confidence: 97%

Arginine Vasopressin (AVP) and AVP-Like Immunoreactivity in Peripheral Tissues

Clements

Funder

1986

Endocrine Reviews

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Section: Other Naturally Occurring Nonapeptidesmentioning

confidence: 97%

Arginine Vasopressin (AVP) and AVP-Like Immunoreactivity in Peripheral Tissues

Clements

Funder

1986

Endocrine Reviews

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“…The receptor was also not activated by mammalian vasopressin; however, it could be activated by vasotocin, oxytocin, isotocin, and Argand Lys-conopressin, although much higher concentrations were needed than for inotocin (EC 50 , Ͼ10 Ϫ6 M). These results indicate that the Ile residue in position 3 present in inotocin and the other five neuropeptides, but absent in vasopressin (Table 1) is more important for receptor activation than the Arg residue in position 8, present in inotocin, vasopressin, vasotocin, and Arg-conopressin, but absent in oxytocin, and isotocin.…”

Section: Cloning Of the Inotocin Preprohormonementioning

confidence: 99%

Cloning and identification of an oxytocin/vasopressin-like receptor and its ligand from insects

Stafflinger

Hansen

Hauser

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

160

156

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However, no similar peptide could be identified in other insects, nor could its prohormone be cloned, or its physiological actions be established. Here, we report that the recently sequenced genome from the red flour beetle Tribolium castaneum contains a gene coding for an oxytocin/vasopressin-like peptide, identical to the locust peptide, which we named inotocin (for insect oxytocin/vasopressin-like peptide) and a gene coding for an inotocin G protein-coupled receptor (GPCR). We cloned the Tribolium inotocin preprohormone and the inotocin GPCR and expressed the GPCR in CHO cells. This GPCR is strongly activated by low concentrations of inotocin (EC50, 5 ؋ 10 ؊9 M), demonstrating that it is the inotocin receptor. Quantitative RT-PCR (qPCR) showed that in adult Tribolium, the receptor is mainly expressed in the head and much less in the hindgut and Malpighian tubules, suggesting that the inotocin/receptor couple does not play a role in water homeostasis. Surprisingly, qPCR also showed that the receptor is 30؋ more expressed in the first larval stages than in adult animals. The inotocin/receptor couple can also be found in the recently sequenced genome from the parasitic wasp Nasonia vitripennis but not in any other holometabolous insect with a completely sequenced genome (12 Drosophila species, the malaria mosquito Anopheles gambiae, the yellow fever mosquito Aedes aegypti, the silk worm Bombyx mori, and the honey bee Apis mellifera), suggesting that this neuropeptide system is confined to basal holometabolous insects. Furthermore, we identified an oxytocin/ vasopressin-like peptide and receptor in the recently sequenced genome from the water flea Daphnia pulex (Crustacea). To our knowledge, this is the first report on the molecular cloning of an oxytocin/vasopressin-like receptor and its ligand from arthropods.inotocin ͉ neuropeptide ͉ GPCR ͉ evolution ͉ reproduction

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“…irAVT, AVT-like immunoreactivity; IT, isotocin ((4-serine, 8-isoleucine]-oxytocin); LVP, [8- both LVP and the recently described analogue phenypressin ([2-phenylalanine]-arginine-vasopressin) (3). Arginine vasotocin (AVT; [8-arginine]-oxytocin), the most ubiquitous neurohypophysial principle in submammalian vertebrates, has been reported in fetal pituitary and pineal glands and/or adult pineal glands of a variety of mammalian species (4)(5)(6)(7)(8)(9).…”

mentioning

confidence: 99%

Arginine vasotocin in ovine fetal blood, urine, and amniotic fluid.

Ervin¹,

Leake²,

Ross³

et al. 1985

J. Clin. Invest.

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Arginine vasotocin (18-argininel-oxytocin) (AVT), the primary antidiuretic principle in submammalian vertebrates, has been reported to be present in mammalian pituitary and pineal glands. Although the most phyletically ubiquitous of the known neurohypophysial peptides, AVT is still not recognized as a mammalian hormone. We examined plasma, urine, and amniotic fluid from fetal lambs by radioimmunoassay (RIA) for evidence of AVT to assess the possibility of its being such a hormone. Measureable quantities of AVT-like immunoreactivity (irAVT) were observed in fetal plasma (2.4±0.2 pg/ml), urine (1.4±0.2 pg/ml), and amniotic fluid (1.9±0.2 pg/ml). Since the AVT antiserum shows minimal cross-reactivity with arginine vasopressin (AVP) and oxytocin (OT), measurements of AVP and OT concentrations in the same biological fluids also were conducted with specific antisera. The results suggest that the irAVT was not accountable on the basis of cross-reaction. To further verify the identity of the irAVT, a high pressure liquid chromatography system using RIA as a means of detection was developed. This system is sufficiently sensitive to allow the separation and quantitation of picogram quantities of the synthetic peptides AVT, AVP, and OT. In this system, the irAVT in fetal plasma, urine, and amniotic fluid appeared as a single peak coeluting with synthetic AVT. These results indicate that AVT is present in ovine fetal plasma and support the view that the fetus secretes this peptide. The physiological significance of circulating AVT remains to be defined.

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Phenypressin (Phe2-Arg8-vasopressin), a new neurohypophysial peptide found in marsupials

Cited by 53 publications

References 11 publications

Arginine Vasopressin (AVP) and AVP-Like Immunoreactivity in Peripheral Tissues

Arginine Vasopressin (AVP) and AVP-Like Immunoreactivity in Peripheral Tissues

Cloning and identification of an oxytocin/vasopressin-like receptor and its ligand from insects

Arginine vasotocin in ovine fetal blood, urine, and amniotic fluid.

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