Phorbol esters isolated from Croton megalobotrys reverse HIV latency ex vivo

Tietjen, Ian; Richard, Khumoekae; Williams, D.; Rivera-Ortiz, Jocelyn; Cai, Yanhui; Pagliuzza, Amélie; Chomont, Nicolas; Andersen, Raymond J.; Montaner, Luis J.; Andrae‐Marobela, Kerstin

doi:10.1016/s2055-6640(20)30191-6

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“…Therefore, TILDA would be an ideal screening tool to validate promising compounds. For instance, TILDA contributed to defining a new class of novel pure phorbol ester-class compounds, isolated from an African medicinal plant (Mukungulu) ( Tietjen et al, 2018 ), with the capacity to promote HIV-1 latency reversal ex-vivo in J-Lat cells containing an HIV-1-GFP provirus ( Tietjen et al, 2019 ). TILDA results could also be used to differentiate T cell populations with large and small inducible HIV-1 reservoirs.…”

Section: Application Of Tilda In Hiv-1 Cure Interventionsmentioning

confidence: 99%

Inducible HIV-1 Reservoir Quantification: Clinical Relevance, Applications and Advancements of TILDA

et al. 2021

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The presence of a stable HIV-1 reservoir persisting over time despite effective antiretroviral suppression therapy precludes a cure for HIV-1. Characterizing and quantifying this residual reservoir is considered an essential prerequisite to develop and validate curative strategies. However, a sensitive, reproducible, cost-effective, and easily executable test is still needed. The quantitative viral outgrowth assay is considered the gold standard approach to quantify the reservoir in HIV-1-infected patients on suppressive ART, but it has several limitations. An alternative method to quantify the viral reservoir following the reactivation of latent HIV-1 provirus detects multiply-spliced tat/rev RNA (msRNA) molecules by real-time PCR [tat/rev induced limiting dilution assay (TILDA)]. This article provides a perspective overview of the clinical relevance, various applications, recent advancements of TILDA, and how the assay has contributed to our understanding of the HIV-1 reservoir.

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Section: Application Of Tilda In Hiv-1 Cure Interventionsmentioning

confidence: 99%