Phosphate binding protein as the biorecognition element in a biosensor for phosphate

Yeast Pif1 Helicase Exhibits a One-base-pair Stepping Mechanism for Unwinding Duplex DNA

“…Cells expressing the E. coli A197C phosphate-binding protein (PBP) were grown as described (25), and the A197C-PBP was purified and labeled with MDCC as described (26).…”

Recent advances in phosphate biosensors

“…In addition to the enzymatic biosensors, few biosensors have been reported in which phosphate-binding proteins were used as the bio-recognition element rather than enzyme system, for phosphate estimation (Salins et al 2004;Okoh et al 2006;Basheer et al 2011). In unicellular eukaryotes, such as yeast, and Gramnegative bacteria, such as E. coli and Salmonella spp., the transport of ligands across the membrane is mediated by some specific proteins, called as periplasmic-binding proteins (PBPs) that bind to the ligand with high affinity (Herrou and Crosson 2013).…”

Biomimetic Molecular Recognition Elements for Chemical Sensing

“…Several members of the periplasmic binding protein superfamily, such as the phosphate-binding protein, have proven to be effective biomolecular materials for detection of their natural targets [170]. Using computational approaches to reengineer the maltose-binding protein, Hellinga and coworkers [171] have shown the capability of altering the specificity of the protein to a number of nonnative substrates including the explosive trinitrotoluene.…”