Phosphate Solubilization by Enterobacter cloacae and its Impact on Growth and Yield of Wheat Plants

Borham, Ali; Belal, Elsayed B.; Metwaly, Metwaly; El-gremy, Shokry Mohammed

doi:10.21608/jsas.2017.1035.1004

Cited by 10 publications

(6 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…and make available the essential nutrient phosphorus to sustain plant growth (34). Reports are on a phosphate solubilising E. cloacae (B1) efficiently enhanced growth characteristics, physiological characteristics as well as yield characteristics of wheat plant that strongly support our findings (35).…”

Section: Isolatessupporting

confidence: 84%

Plant growth promoting and antagonistic Enterobacter sp. EPR4 from common bean rhizosphere of garhwal himalayan inhibits a soil-borne pathogen Sclerotinia sclerotiorum

Kumar

Dubey²,

Kumar³

2022

Plant Sci. Today

View full text Add to dashboard Cite

Plant growth stimulating and antagonistic properties of 7 bacterial isolates of beneficial Enterobacter spp. (EPR1- EPR7) screened from the rhizospheric soil of Phaseolus vulgaris plants growing in Garhwal Himalaya, Uttarakhand, India was studied against soil borne phytopathogen Sclerotinia sclerotiorum causes root rots in various crops. Among the isolates, EPR4 showed 64.8% reduction in colony growth of the fungal pathogen in dual culture. All seven isolates are capable of producing Indole Acetic Acid (IAA), but EPR4 also produced cyanogens, solubilized inorganic and organic phosphate, siderophore, ACC (1-aminocyclopropane-1-carboxylic acid) deamininase, and extracellular enzymes like chitinase which inhibited the phytopathogen. For the EPR4 strain, 16S rRNA gene sequencing was followed by NCBI - BLAST similarity showed the maximum sequence similarity (100%) with the species of Enterobacter (available on NCBI data base), and recognized as Enterobacter sp. EPR4 (GenBank accession number JN225424). The Enterobacter sp. EPR4 has the potential to be used as a biocontrol agent against S. sclerotiorum as well as a good plant growth promoter for common bean and other crops grown in India's Garhwal Himalaya.

show abstract

Section: Isolatessupporting

confidence: 84%

Plant growth promoting and antagonistic Enterobacter sp. EPR4 from common bean rhizosphere of garhwal himalayan inhibits a soil-borne pathogen Sclerotinia sclerotiorum

Kumar

Dubey²,

Kumar³

2022

Plant Sci. Today

View full text Add to dashboard Cite

show abstract

“…Pan et al [60] similarly observed that Enterobacter ludwigii inoculated on tomato seeds promoted their growth and improved their foliar protein properties and the elongation of their roots and stem. Borham et al [61] evaluated the effect of a strain of Enterobacter on wheat crops, inoculating it with or without fertilizers. The bacterium showed to increase stem elongation and chlorophyll production in the crop, and also an improvement in fertilizer utilization.…”

Section: Discussionmentioning

confidence: 99%

Inorganic Phosphate Solubilization by a Novel Isolated Bacterial Strain Enterobacter sp. ITCB-09 and Its Application Potential as Biofertilizer

et al. 2020

View full text Add to dashboard Cite

The excessive use of fertilizers in agriculture is mainly due to the recognized plant requirements for soluble phosphorus. This problem has limited the implementation of sustainable agriculture. A viable alternative is to use phosphate solubilizing soil microorganisms. This work aimed to isolate inorganic phosphorus-solubilizing bacteria from the soils of agroecosystems, to select and identify, based on sequencing and phylogenetic analysis of the 16S rRNA gene, the bacterium with the highest capacity for in vitro solubilization of inorganic phosphate. Additionally, we aimed to determine its primary phosphate solubilizing mechanisms and to evaluate its effect on Habanero pepper seedlings growth. A total of 21 bacterial strains were isolated by their activity on Pikovskaya agar. Of these, strain ITCB-09 exhibited the highest ability to solubilize inorganic phosphate (865.98 µg/mL) through the production of organic acids. This strain produced extracellular polymeric substances and siderophores that have ecological implications for phosphate solubilization. 16S rRNA gene sequence analysis revealed that strain ITCB-09 belongs to the genus Enterobacter. Enterobacter sp. ITCB-09, especially when immobilized in beads, had a positive effect on Capsicum chinense Jacq. seedling growth, indicating its potential as a biofertilizer.

show abstract

“…Accordingly, under conditions of low-temperature stress, the protective effect of K. planticola TSKhA-91 on the development of cucumber seeds and stimulation of germination and root formation by its seeds were shown. Similarly, Borham et al [22] were evaluated phosphate solubilization effects of Enterobacter cloacae (strain B1) through its impact on growth and yield characteristics of wheat, cultivar Masr 1, in comparison with chemical fertilizers (phosphoric acid, calcium superphosphate, and NPK), under field conditions. Results of their study showed that E. cloacae (B1) effectively increased growth characteristics including plant height, fresh weight, dry weight, flag leaf area, and physiological characteristics including chlorophyll pigments content and yield characteristics such as spike length, spikelets number, grains number/spike, 1000 kernels weight, spike weight, biological weight and grains weight, in comparison with control treatment.…”

Section: Introductionmentioning

confidence: 99%

Case Study upon Foliar Application of Biofertilizers Affecting Microbial Biomass and Enzyme Activity in Soil and Yield Related Properties of Maize and Wheat Grains

Latković

Maksimović²,

Dinić³

et al. 2020

Biology

View full text Add to dashboard Cite

This study evaluated the effects of the application of microbial inoculants (N-fixing Klebsiella planticola and Enterobacter spp.), two rates of composite mineral fertilizers, and their combination on microbial biomass carbon (MBC), dehydrogenase (DHA), and proteinase activity (PTA) in Lessivated Cambisol and yield-related properties of maize and wheat grains in a two-year trial. Unfertilized soil was used as a control variant. MBC was measured using the chloroform fumigation-extraction method, DHA was determined spectrophotometrically by measuring the intensity of the formed red-colored triphenyl formazan, while PTA was determined using a titration method by measuring the degree of gelatine decomposition. In grain samples, P was determined spectrophotometrically, K—by flame emission photometry, N—on an elemental carbon/nitrogen/sulfur (CNS) analyzer, and crude proteins—by calculation of N content. Measuring both crops’ yield was carried out at the end of the vegetation. The results indicated that mineral fertilizers are not, in general, negative for soil microbiota when used in the context of sustainable agriculture without monoculture. There is a significant increase in the values of soil MBC, DHA, and PTA in the variants with combined application of bacterial inoculants and lower rates of mineral fertilizers. The highest values of these parameters were determined in the period with a better distribution of precipitation during the vegetation period of the year. The mentioned combination also resulted in a higher grain yield of maize and wheat comparing to the application of lower rates of the NPK nutrients solely. The combined application of high rates of mineral fertilizers and bacterial inoculants resulted in significantly increased N, P, K, and protein content in the grains of crops, and the same applied to yield. Concluding, studied bacterial inoculants can be used to specify the replacement of nitrogen fertilizers, stimulating the microbial biomass and enzyme activity in the soil, helping to ensure that the supply of nutrients contributing to an optimized yield of crops is maintained.

show abstract

Phosphate Solubilization by Enterobacter cloacae and its Impact on Growth and Yield of Wheat Plants

Cited by 10 publications

References 25 publications

Plant growth promoting and antagonistic Enterobacter sp. EPR4 from common bean rhizosphere of garhwal himalayan inhibits a soil-borne pathogen Sclerotinia sclerotiorum

Plant growth promoting and antagonistic Enterobacter sp. EPR4 from common bean rhizosphere of garhwal himalayan inhibits a soil-borne pathogen Sclerotinia sclerotiorum

Inorganic Phosphate Solubilization by a Novel Isolated Bacterial Strain Enterobacter sp. ITCB-09 and Its Application Potential as Biofertilizer

Case Study upon Foliar Application of Biofertilizers Affecting Microbial Biomass and Enzyme Activity in Soil and Yield Related Properties of Maize and Wheat Grains

Contact Info

Product

Resources

About