Phosphatidic Acid Plays a Central Role in the Transcriptional Regulation of Glycerophospholipid Synthesis in Saccharomyces cerevisiae

Carman, George M.; Henry, Susan A.

doi:10.1074/jbc.r700038200

Cited by 195 publications

(294 citation statements)

References 85 publications

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“…Since local concentration of Ca 2þ and Mg 2þ can fluctuate very rapidly in particular cellular condition, this chemical property of PA is an important feature to probe its environment. As a consequence PA can behave as a pH biosensor as notably reported in yeast for regulation of expression of phospholipid metabolic genes through PA-dependent sequestration in the ER of the transcription factor Opi1p [16,51,52]. Interestingly it has been recently demonstrated that during the first minutes of carbon deprivation the cytosolic pH of plant cells rises from 7.4 to stabilize at 7.8 and recovers to more acidic pH within 5 min after carbon repletion accompanying modification of level of soluble phosphorous metabolites [53].…”

Section: Mode Of Action Of Phosphatidic Acid In Signalling Eventsmentioning

confidence: 87%

“…PA formed by ER acyltransferases can generate phospholipids via 2 different pathways: i) the CDP-DAG pathway and ii) the Kennedy pathway through dephosphorylation of PA into DAG. In yeast, both pathways generate PC and PE [16]. The CDP-DAG pathway is more active in the growth phase but nutrient depletion induces a shift towards the Kennedy pathway [16].…”

Section: Phosphatidic Acid As An Intermediate Metabolite In Galactolimentioning

confidence: 99%

“…In yeast, both pathways generate PC and PE [16]. The CDP-DAG pathway is more active in the growth phase but nutrient depletion induces a shift towards the Kennedy pathway [16]. The Kennedy pathway indeed contributes to recycling the polar head of phospholipids such as choline and ethanolamine [20] According to [21] Leaf Root Whole seedling ATS1 Glycerol-3-phosphate acyltransferase through phospholipases D (PLDs) and eventually to remodelling of membranes.…”

Section: Phosphatidic Acid As An Intermediate Metabolite In Galactolimentioning

confidence: 99%

“…The Kennedy pathway indeed contributes to recycling the polar head of phospholipids such as choline and ethanolamine [20] According to [21] Leaf Root Whole seedling ATS1 Glycerol-3-phosphate acyltransferase through phospholipases D (PLDs) and eventually to remodelling of membranes. The PA phosphatase Pah1p is critical for the shift from the CDP-DAG to the Kennedy pathway because it catalyzes the hydrolysis of PA into DAG, and furthermore because the Pah1p-produced DAG negatively regulates the level of expression of genes encoding the CDP-DAG pathway [16]. In plants, the biosynthetic pathways show similarity with yeast but also major differences partly related with galactolipid synthesis.…”

Section: Phosphatidic Acid As An Intermediate Metabolite In Galactolimentioning

confidence: 99%

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Role of phosphatidic acid in plant galactolipid synthesis

Dubots¹,

Botté²,

Boudière³

et al. 2012

Biochimie

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Phosphatidic acid (PA) is a precursor metabolite for phosphoglycerolipids and also for galactoglycerolipids, which are essential lipids for formation of plant membranes. PA has in addition a main regulatory role in a number of developmental processes notably in the response of the plant to environmental stresses. We review here the different pools of PA dispatched at different locations in the plant cell and how these pools are modified in different growth conditions, particularly during plastid membrane biogenesis and when the plant is exposed to phosphate deprivation. We analyze how these modifications can affect galactolipid synthesis by tuning the activity of MGD1 enzyme allowing a coupling of phosphoand galactolipid metabolisms. Some mechanisms are considered to explain how physicochemical properties of PA allow this lipid to act as a central internal sensor in plant physiology.

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Section: Mode Of Action Of Phosphatidic Acid In Signalling Eventsmentioning

confidence: 87%

Section: Phosphatidic Acid As An Intermediate Metabolite In Galactolimentioning

confidence: 99%

Section: Phosphatidic Acid As An Intermediate Metabolite In Galactolimentioning

confidence: 99%

Section: Phosphatidic Acid As An Intermediate Metabolite In Galactolimentioning

confidence: 99%

See 2 more Smart Citations

Role of phosphatidic acid in plant galactolipid synthesis

Dubots¹,

Botté²,

Boudière³

et al. 2012

Biochimie

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“…The first identified yeast bHLH protein, Pho4p, forms a homodimer required for the induction of phosphate utilization genes when phosphate levels are low (Berben et al, 1990;Johnston and Carlson, 1992;Oshima, 1997). The Ino2p : Ino4p heterodimer induces phospholipid biosynthetic gene expression in response to inositol deprivation (Carman and Han, 2008;Carman and Henry, 2007;. The Sgc1p homodimer activates expression of glycolytic genes (e.g.…”

Section: Introductionmentioning

confidence: 99%

Multiple bHLH proteins regulate CIT2 expression in Saccharomyces cerevisiae

Chen¹,

Lopes²

2010

Yeast

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The basic helix-loop-helix (bHLH) proteins comprise a eukaryotic transcription factor family involved in multiple biological processes. They have the ability to form multiple dimer combinations and most of them also bind a 6 bp site (E-box) with limited specificity. These properties make them ideal for combinatorial regulation of gene expression. The Saccharomyces cerevisiae CIT2 gene, which encodes citrate synthase, was previously known to be induced by the bHLH proteins Rtg1p and Rtg3p in response to mitochondrial damage. Rtg1p-Rtg3p dimers bind two R-boxes (modified E-boxes) in the CIT2 promoter. The current study tested the ability of all nine S. cerevisiae bHLH proteins to regulate the CIT2 gene. The results showed that expression of CIT2-lacZ reporter was induced in a ρ 0 strain by the presence of inositol via the Ino2p and Ino4p bHLH proteins, which are known regulators of phospholipid synthesis. Promoter mutations revealed that inositol induction required a distal E-box in the CIT2 promoter. Interestingly, deleting the INO2, INO4 genes or the cognate E-box revealed phosphate induction of CIT2 expression. This layer of expression required the two R-boxes and the Pho4p bHLH protein, which is known to be required for phosphate-specific regulation. Lastly, the data show that the Hms1p and Sgc1p bHLH proteins also play important roles in repression of CIT2-lacZ expression. Collectively, these results support the model that yeast bHLH proteins coordinate different biological pathways.

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Molecular and Cellular Mechanisms for Lipid Synthesis and Accumulation in Microalgae: Biotechnological Implications

Han

Yoon

et al. 2013

Handbook of Microalgal Culture

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Phosphatidic Acid Plays a Central Role in the Transcriptional Regulation of Glycerophospholipid Synthesis in Saccharomyces cerevisiae

Cited by 195 publications

References 85 publications

Role of phosphatidic acid in plant galactolipid synthesis

Role of phosphatidic acid in plant galactolipid synthesis

Multiple bHLH proteins regulate CIT2 expression in Saccharomyces cerevisiae

Molecular and Cellular Mechanisms for Lipid Synthesis and Accumulation in Microalgae: Biotechnological Implications

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