Phosphofructokinase in rat lung during perinatal development: characterization of subunit composition and regulation by fructose 2,6-bisphosphate and glucose 1,6-bisphosphate

Phosphofructokinase and pyruvate kinase in mouse embryonal carcinoma P19 cells in relation to growth and differentiation

“…The cells were disrupted by ultrasonication at 4" C with a Branson sonifier (50 Watts, 2 x 10 s) and subsequently centrifuged for 30 min at 48.000 g (4" C). The activity of the enzyme was assayed under Vmax conditions (37" C. pH 8.0) as described previously [7]. One unit (U) of PFK activity is defined as the amount of enzyme converting 1 pmol fructose 6-phosphate into fructose 1,6-bisphosphate per min at 37" C.…”