Phosphoinositide-specific Phospholipase C β1 gene deletion in bipolar disorder affected patient

Vasco, Vincenza Rita Lo; Longo, Lucia; Polonia, Patrizia

doi:10.1007/s12079-012-0182-2

Cited by 32 publications

(25 citation statements)

References 30 publications

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“…RISC is a ribonucleoprotein complex that silences gene expression by cleaving and degrading mRNA. Its activity is initiated by the RNase III enzyme Dicer to process dsRNA into ~22 nucleotide microRNA's (miRNA or miR) . For degradation to happen, a guide strand of miRNA acts as a template for a complementary mRNA passenger strand to bind.…”

Section: Plcβ Inhibits the Promoter Of Rna‐induced Silencing C3po Tmentioning

confidence: 99%

Regulation of bifunctional proteins in cells: Lessons from the phospholipase Cβ/G protein pathway

et al. 2019

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Some proteins can serve multiple functions depending on different cellularconditions. An example of a bifunctional protein is inositide-specific mammalian phospholipase Cβ (PLCβ). PLCβ is activated by G proteins in response to hormones and neurotransmitters to increase intracellular calcium. Recently, alternate cellular function(s) of PLCβ have become uncovered. However, the conditions that allow these different functions to be operative are unclear. Like many mammalian proteins, PLCβ has a conserved catalytic core along with several regulatory domains. These domains modulate the intensity and duration of calcium signals in response to external sensory information, and allow this enzyme to inhibit protein translation in a noncatalytic manner. In this review, we first describe PLCβ's cellular functions and regulation of the switching between these functions, and then discuss the thermodynamic considerations that offer insight into how cells manage multiple and competitive associations allowing them to rapidly shift between functional states.

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Section: Plcβ Inhibits the Promoter Of Rna‐induced Silencing C3po Tmentioning

confidence: 99%

Regulation of bifunctional proteins in cells: Lessons from the phospholipase Cβ/G protein pathway

et al. 2019

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“…In fact, it is highly expressed in the cerebral cortex, hippocampus, amygdala, lateral septum, and olfactory bulb ( 67,68 ), where it regulates both cortical development and synaptic plasticity by specifi cally modulating hippocampal muscarinic acetylcholine receptor signaling. Moreover, a PI-PLC ␤ 1 gene deletion was observed in orbito-frontal cortex samples from human patients with schizophrenia and bipolar disorders (69)(70)(71), and patients with these diseases also showed an abnormal expression pattern of PI-PLC ␤ 1 in specifi c brain areas ( 66 ).…”

Section: Pi-plc In Diseasementioning

confidence: 99%

Phosphoinositide-specific phospholipase C in health and disease

Cocco

Follo

Manzoli

et al. 2015

Journal of Lipid Research

140

100

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“…Knowledge of the expression panels of PI-PLC enzymes is a necessary and preliminary tool to address studies about their role in quiescent cells. Moreover, the expression panels of PI-PLC enzymes have been demonstrated to differ in quiescent cells with respect to the pathological or activated counterpart (18)(19)(20)(21)(22)(23)(24)(25)(26)(27)(28)(29)(30).…”

Section: Introductionmentioning

confidence: 99%

Comparison of Phosphoinositide-Specific Phospholipase C Expression Panels of Human Osteoblasts Versus MG-63 and Saos Osteoblast-Like Cells

Vasco¹,

Leopizzi²,

d’Abusco³

et al. 2016

Avicenna J Med Biochem

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Background: A large number of phospholipase C (PLC) enzymes, both mRNA transcripts and proteins, have been detected in osteoblasts, corroborating the importance of calcium regulation in bone tissue. MG-63 and SaOS-2 human osteosarcoma cell lines are actually considered osteoblast-like cells, and are therefore widely used as experimental models for osteoblasts. Objectives: Our aim was to verify whether MG-63 or SaOS-2 cells might also represent appropriate experimental osteoblast models for signal transduction studies, with special regard to the phosphoinositide (PI) pathway. We analyzed the expression and the subcellular distribution of enzymes related to calcium signal transduction (the PI-specific PLC family), which are known to possess high cell/tissue specificity. Materials and Methods:The expression of PLC genes was analyzed by performing RT-PCR experiments. The presence of PLC enzymes and their subcellular distribution within the cells was analyzed with immunofluorescence experiments. Results: Osteoblasts, MG-63 cells, and SaOS-2 cells have expression panels similar to those of PLC enzymes. However, slight differences were found in the expression of enzymes belonging to the PLCη subfamily. Conclusions: MG-63 and SaOS-2 osteosarcoma cell lines might not represent appropriate experimental models for studies that aim to analyze signal transduction in osteoblasts.

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Phosphoinositide-specific Phospholipase C β1 gene deletion in bipolar disorder affected patient

Cited by 32 publications

References 30 publications

Regulation of bifunctional proteins in cells: Lessons from the phospholipase Cβ/G protein pathway

Regulation of bifunctional proteins in cells: Lessons from the phospholipase Cβ/G protein pathway

Phosphoinositide-specific phospholipase C in health and disease

Comparison of Phosphoinositide-Specific Phospholipase C Expression Panels of Human Osteoblasts Versus MG-63 and Saos Osteoblast-Like Cells

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