Phosphoinositides: Important lipids in the coordination of cell dynamics

Viaud, Julien; Mansour, Rana; Antkowiak, Adrien; Mujalli, Abdulrahman; Valet, Colin; Chicanne, Gaëtan; Xuereb, Jean-Marie; Terrisse, Anne-Dominique; Séverin, Sonia; Gratacap, Marie-Pierre; Gaits‐Iacovoni, Frédérique; Payrastre, Bernard

doi:10.1016/j.biochi.2015.09.005

Cited by 82 publications

(84 citation statements)

References 97 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The different PI species present in human and yeast cells are shown in Fig. A,B, respectively . In addition to the PI kinases mentioned above, the human genome has three APIM‐containing PI kinases (PIK3C2B, PIK3CA, and PIK3CG) (Fig.…”

Section: Resultsmentioning

confidence: 99%

The role of PCNA as a scaffold protein in cellular signaling is functionally conserved between yeast and humans

et al. 2018

View full text Add to dashboard Cite

Proliferating cell nuclear antigen ( PCNA ), a member of the highly conserved DNA sliding clamp family, is an essential protein for cellular processes including DNA replication and repair. A large number of proteins from higher eukaryotes contain one of two PCNA ‐interacting motifs: PCNA ‐interacting protein box ( PIP box) and AlkB homologue 2 PCNA ‐interacting motif ( APIM ). APIM has been shown to be especially important during cellular stress. PIP box is known to be functionally conserved in yeast, and here, we show that this is also the case for APIM . Several of the 84 APIM ‐containing yeast proteins are associated with cellular signaling as hub proteins, which are able to interact with a large number of other proteins. Cellular signaling is highly conserved throughout evolution, and we recently suggested a novel role for PCNA as a scaffold protein in cellular signaling in human cells. A cell‐penetrating peptide containing the APIM sequence increases the sensitivity toward the chemotherapeutic agent cisplatin in both yeast and human cells, and both yeast and human cells become hypersensitive when the Hog1/p38 MAPK pathway is blocked. These results suggest that the interactions between APIM ‐containing signaling proteins and PCNA during the DNA damage response is evolutionary conserved between yeast and mammals and that PCNA has a role in cellular signaling also in yeast.

show abstract

Section: Resultsmentioning

confidence: 99%

The role of PCNA as a scaffold protein in cellular signaling is functionally conserved between yeast and humans

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Leashing the turtle with a diacylglycerol (DAG) moiety through a phosphodiester bond on the right flipper (number 1) generates phosphatidylinositol (PtdIns) the precursor to seven phosphorylated derivatives (phosphoinositides) (Figure 1). These can be interconverted by a series of lipid kinases and phosphatases [2]. Unlike the myo-inositol phosphates which are water soluble, PtdIns and its phosphorylated derivatives with their hydrophobic tails much prefer to swim within a membrane.…”

Section: Turtles Everywherementioning

confidence: 99%

“…Phosphorylation is indicated by the white pebble. (B) Nuclei were isolated from control murine erythroleukemia (MEL) cells (1) or from cells differentiated down the erythroid pathway (2). Nuclei were incubated with 32 P-ATP and the labelled lipids were extracted and separated by TLC.…”

Section: Figurementioning

confidence: 99%

Phosphoinositides in the nucleus and myogenic differentiation: how a nuclear turtle with a PHD builds muscle

Divecha

2016

Biochemical Society Transactions

View full text Add to dashboard Cite

Phosphoinositides are a family of phospholipid messenger molecules that control various aspects of cell biology in part by interacting with and regulating downstream protein partners. Importantly, phosphoinositides are present in the nucleus. They form part of the nuclear envelope and are present within the nucleus in nuclear speckles, intra nuclear chromatin domains, the nuclear matrix and in chromatin. What their exact role is within these compartments is not completely clear, but the identification of nuclear specific proteins that contain phosphoinositide interaction domains suggest that they are important regulators of DNA topology, chromatin conformation and RNA maturation and export. The plant homeo domain (PHD) finger is a phosphoinositide binding motif that is largely present in nuclear proteins that regulate chromatin conformation. In the present study I outline how changes in the levels of the nuclear phosphoinositide PtdIns5P impact on muscle cell differentiation through the PHD finger of TAF3 (TAF, TATA box binding protein (TBP)-associated factor), which is a core component of a number of different basal transcription complexes.

show abstract

“…Phosphatidylinositols (PI) represent another class of GPLs that have been found to be involved with lipid and cell signaling, as well as regulation of membrane proteins and membrane trafficking. 14,15 PIs show a high diversity in the head group structure, especially since the hydroxyl groups of inositol can be phosphorylated at positions 3, 4, and 5 to generate any of three PI monophosphates (PI(3)P, PI(4)P, PI(5)P), three bisphosphates (PI(3,4)P 2 , PI(3,5)P 2 , PI(4,5)P 2 ), or one triphosphate (PI(3,4,5)P 3 ). PIs are mostly absent in prokaryotic cells.…”

Section: à440mentioning

confidence: 99%