Phosphoinositides in Cell Architecture

Shewan, Annette M.; Eastburn, Dennis J.; Mostov, Keith E.

doi:10.1101/cshperspect.a004796

Cited by 173 publications

(163 citation statements)

References 134 publications

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“…This tight control of PIP3 levels is likely to be extremely important as PIP3 regulates a wide variety of cellular processes including growth, proliferation and apicobasal organization (Shewan et al, 2011;Vanhaesebroeck et al, 2012). The absence of Pten from DC and wound leading edges permits PIP3 to become elevated specifically in these locations.…”

Section: Discussionmentioning

confidence: 99%

“…PI3K catalyses the phosphorylation of phosphatidylinositol 4,5-bisphosphate [PIP2; PtdIns(4,5)P 2 ] to yield PIP3 while Pten catalyses the reverse reaction, dephosphorylating PIP3 to PIP2. Phosphoinositides are also known to regulate the assembly and apicobasal organization of cell-cell junctions in epithelia, and both PI3K and Pten can associate with cell junction (Kovacs et al, 2002;Shewan et al, 2011;von Stein et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

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Par3/Bazooka and phosphoinositides regulate actin protrusion formation during Drosophila dorsal closure and wound healing

et al. 2013

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SUMMARYEffective wound closure mechanisms are essential for maintenance of epithelial structure and function. The repair of wounded epithelia is primarily driven by the cells bordering the wound, which become motile after wounding, forming dynamic actin protrusions along the wound edge. The molecular mechanisms that trigger wound edge cells to become motile following tissue damage are not well understood. Using wound healing and dorsal closure in Drosophila, we identify a direct molecular link between changes in cell-cell adhesion at epithelial edges and induction of actin protrusion formation. We find that the scaffolding protein Par3/Bazooka and the lipid phosphatase Pten are specifically lost from cell-cell junctions at epithelial edges. This results in a localized accumulation of phosphatidylinositol 3,4,5-trisphosphate (PIP3), which promotes the formation of actin protrusions along the epithelial edge. Depleting PIP3 results in defective epithelial closure during both dorsal closure and wound healing. These data reveal a novel mechanism that directly couples loss of epithelial integrity to activation of epithelial closure.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Par3/Bazooka and phosphoinositides regulate actin protrusion formation during Drosophila dorsal closure and wound healing

et al. 2013

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“…This pathway was described as being responsible for stimulating protein kinase (PK) activation, including mitogen-activated kinase protein (MAPK), which has been previously reported as an attendee in the regulation of oocyte meiotic resumption, in which it may act directly or indirectly [9]. Recent studies have shown that MAPK is required in gonadotropininduced meiotic resumption [35,38,39,44]. Other studies have also established that MAPK as well as protein kinase A (PKA) interrupts the communication between the CCs and the oocyte, thus decreasing the cyclic adenosine Fig.…”

Section: Discussionmentioning

confidence: 99%

The follicular microenviroment as a predictor of pregnancy: MALDI-TOF MS lipid profile in cumulus cells

Montani

Cordeiro

Regiani

et al. 2012

J Assist Reprod Genet

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Purpose This research proposed to study the changes in lipid composition in cumulus cells (CCs) from women who achieved pregnancy compared with women who did not, after in vitro fertilization treatment. This approach has the potential to provide novel information on the lipid metabolism of the CCs and as an additional method to predict pregnancy. Method Fifty-four samples from couples with tubal and male factor infertility and where the female partner was age 35 or younger were divided in two groups according to their level of hCG 14 days after embryo transfer as follows: (1) 23 samples in pregnant group and (2) 31 samples in non-pregnant group. Lipid extraction was performed by the Bligh-Dyer protocol, and lipid profiles were obtained by MALDI-TOF MS. Mass spectra data were processed with MassLynx, and statistical analysis was performed using MarkerLynx extended statistic. OPLS-DA model was built. Results S-plot Analysis revealed three ions as potential markers in the pregnant group, and five ions in the nonpregnant group. These ions were identified in the human metabolome database (HMDB) as phosphatidylcholine in the pregnant group and as phosphatidylethanolamine, phosphatidylserine and phosphatidylinositol species in the non-pregnant group. These lipids might be involved in cell proliferation and differentiation, apoptosis and GAP junction regulation. Conclusion We conclude that MALDI-TOF MS can be used as an informative and fast analytical strategy to obtain and study the lipid profile of cumulus cells and can potentially be used as a supporting tool to predict pregnancy based on the metabolic state of the CCs.

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“…Phosphatidylinositol phosphates (PIPs) are important for actin cytoskeleton organization, vesicle trafficking (exo-and endocytosis) and transcription -all of which are required for polarized cell growth (Di Paolo and De Camilli, 2006;Strahl and Thorner, 2007;Vicinanza et al, 2008;Kwiatkowska, 2010;Saarikangas et al, 2010;Shewan et al, 2011). The phosphatidylinositol PtdIns(4,5)P 2 is a major PIP species, generated by phosphorylation of PtdIns(4)P at position 5 by type I phosphatidylinositol-4-phosphate 5-kinases (PI4P5K).…”

Section: Introductionmentioning

confidence: 99%

Phosphoinositide-bis-phosphate is required for Saccharomyces cerevisiae invasive growth

Guillas

Vernay

Vitagliano

et al. 2013

Journal of Cell Science

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SummaryPhosphatidylinositol phosphates are important regulators of processes such as the cytoskeleton organization, membrane trafficking and gene transcription, which are all crucial for polarized cell growth. In particular, phosphatidylinositol 4,5-bisphosphate [PtdIns(4,5)P 2 ] has essential roles in polarized growth as well as in cellular responses to stress. In the yeast Saccharomyces cerevisiae, the sole phosphatidylinositol-4-phosphate 5-kinase (PI4P5K) Mss4p is essential for generating plasma membrane PtdIns(4,5)P 2 . Here, we show that Mss4p is required for yeast invasive growth in low-nutrient conditions. We isolated specific mss4 mutants that were defective in cell elongation, induction of the Flo11p flocculin, adhesion and cell wall integrity. We show that mss4-f12 cells have reduced plasma membrane PtdIns(4,5)P 2 levels as well as a defect in its polarized distribution, yet Mss4-f12p is catalytically active in vitro. In addition, the Mss4-f12 protein was defective in localizing to the plasma membrane. Furthermore, addition of cAMP, but not an activated MAPKKK allele, partially restored the invasive growth defect of mss4-f12 cells. Taken together, our results indicate that plasma membrane PtdIns(4,5)P 2 is crucial for yeast invasive growth and suggest that this phospholipid functions upstream of the cAMP-dependent protein kinase A signaling pathway.

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Phosphoinositides in Cell Architecture

Cited by 173 publications

References 134 publications

Par3/Bazooka and phosphoinositides regulate actin protrusion formation during Drosophila dorsal closure and wound healing

Par3/Bazooka and phosphoinositides regulate actin protrusion formation during Drosophila dorsal closure and wound healing

The follicular microenviroment as a predictor of pregnancy: MALDI-TOF MS lipid profile in cumulus cells

Phosphoinositide-bis-phosphate is required for Saccharomyces cerevisiae invasive growth

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