Phospholipase A₂ from Crotalus atrox venom. I. Purification and some properties

Abstract

Phospholipase A2 (phosphatide acylhydrolase, EC 3.1.

Phospholipase B activity in mycelia of Aspergillus niger

“…A Km(app .... t) of 25 mM was obtained. This is of the same order as those values reported for phospholipase A n activities in R. arrhizus and M. /avanicus mycelia [1] and for snake venom phospholipase A 2 [13] using ethereal substrate solutions. It is possible that such high Km~ap p .... t) values may be due to the physicochemical characteristics of organic solvent reaction systems used [1].…”

Purification of a Nontoxic Phospholipase A2 From the Venom of Indian Krait (Bungarus Caeruleus)

“…The recovery was 37.3% against 66% and 51% yields obtained with Naja naja (Braganca et al, 1969) and Crotalus adamenteus (Wells and Hanahan, 1969) venoms, respectively. Lower yields have been reported with Crotalus atrox (Wu and Tinker, 1969) and Agkistrodon piscivorus (Augustyn and Elliot, 1970). We achieved about 38-fold purification as compared with 25-fold reported from Naja naja (Braganca, 1969), Crotalus adamenteus (Wells and Hanahan, 1969) and Bothrops neuwiedii (Vidal and Stoppani, 1971) venoms.…”

“…3.1.1.4.] which catalyzes the hydrolysis of diacyl phosphoglycerides into their monoacyl derivatives with the release of free fatty acids from the 2 position of glyceride has been isolated from various snake venoms (Braganca, Sambrey and Ghadially, 1969;Wells and Hanahan, 1969;Wu and Tinker, 1969;Tu, Passey and Toom, 1970;Augustyn and Elliot, 1970;Vidal and Stoppani, 1971;Kawauchi et al, 1971;Howard, 1975). PhA2 has also been isolated from pancreatic juice (DeHaas et al, 1968) and from bee venom (Shipolini et al, 1971).…”

Phosphoglyceride Metabolism

“…Accordingly, homogeneous enzymes have been obtained from the snakes Crotalus adamanteus (144,145), Crotalus atrox (146,147), Naja naja (148,149), Naja nigrieollis (150), Agkistrodon piscivorus (151), Aykistrodon halys blomhoffi (152), Laticauda semifasciata (153), and from the honeybee Apis melli[ica (154,155). Accordingly, homogeneous enzymes have been obtained from the snakes Crotalus adamanteus (144,145), Crotalus atrox (146,147), Naja naja (148,149), Naja nigrieollis (150), Agkistrodon piscivorus (151), Aykistrodon halys blomhoffi (152), Laticauda semifasciata (153), and from the honeybee Apis melli[ica (154,155).…”