Phospholipase A2 Crotoxin B Isolated from the Venom of Crotalus durissus terrificus Exert Antiviral Effect against Dengue Virus and Yellow Fever Virus through Its Catalytic Activity

Russo, Raquel Rinaldi; Mller, Vanessa Danielle Menjon; Cintra, Adélia Cristina Oliveira; Figueiredo, Luíz Tadeu Moraes; Sampaio, Suely Vilela; Aquino, Víctor Hugo

doi:10.4172/2324-8955.1000121

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“…More recently, the effect of PLA 2 -CB against DENV was associated to its catalytic activity, inactivating virus particle probably by cleavage of glycerophospholipid of the virus envelope [ 52 ]. These results were also described for other enveloped virus such as Rocio virus , Oropouche virus , Mayaro virus [ 16 ] and Human Immunodeficiency Virus (HIV) [ 15 ].…”

Section: Discussionmentioning

confidence: 99%

Multiple effects of toxins isolated from Crotalus durissus terrificus on the hepatitis C virus life cycle

et al. 2017

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Hepatitis C virus (HCV) is one of the main causes of liver disease and transplantation worldwide. Current therapy is expensive, presents additional side effects and viral resistance has been described. Therefore, studies for developing more efficient antivirals against HCV are needed. Compounds isolated from animal venoms have shown antiviral activity against some viruses such as Dengue virus, Yellow fever virus and Measles virus. In this study, we evaluated the effect of the complex crotoxin (CX) and its subunits crotapotin (CP) and phospholipase A2 (PLA2-CB) isolated from the venom of Crotalus durissus terrificus on HCV life cycle. Huh 7.5 cells were infected with HCVcc JFH-1 strain in the presence or absence of these toxins and virus was titrated by focus formation units assay or by qPCR. Toxins were added to the cells at different time points depending on the stage of virus life cycle to be evaluated. The results showed that treatment with PLA2-CB inhibited HCV entry and replication but no effect on HCV release was observed. CX reduced virus entry and release but not replication. By treating cells with CP, an antiviral effect was observed on HCV release, the only stage inhibited by this compound. Our data demonstrated the multiple antiviral effects of toxins from animal venoms on HCV life cycle.

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Section: Discussionmentioning

confidence: 99%