Phospholipase A2: The key to reversing long-term memory impairment in a gastropod model of aging

Watson, Shawn; Wright, Natasha; Hermann, Petra M.; Wildering, Willem C.

doi:10.1016/j.neurobiolaging.2012.02.028

Cited by 12 publications

(62 citation statements)

References 64 publications

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“…Prior studies in L. stagnalis implicate natural and experimentally (AAPH) induced non-enzymatic lipid peroxidation to memory deficiencies in an aversive operant conditioning of aerial respiratory behaviour [7]. We also demonstrated this phenomenon can be fully reversed through treatment with the general PLA 2 inhibitor aristolochic acid [7].…”

Section: Resultsmentioning

confidence: 54%

“…We also demonstrated this phenomenon can be fully reversed through treatment with the general PLA 2 inhibitor aristolochic acid [7]. Therefore, we now proceed with testing whether AAPH treatment also affect non-aversive appetitive classical conditioning.…”

Section: Resultsmentioning

confidence: 99%

“…The present study follows from our recent observations implicating age-, inflammation- and experimental oxidative stress-induced PLA 2 activity in long-term memory (LTM) failure [7,22]. Considering the pivotal role of PLA 2 activation in behavioural plasticity, we investigated the question whether it is the fatty acids released upon PLA 2 activation that causes cognitive impairment in our model system.…”

Section: Introductionmentioning

confidence: 97%

“…Our current research on the foundations of learning and memory impairment in the pond snail Lymnaea stagnalis ( L. stagnalis; L. ) focuses on the question of lipid-peroxidation dependent facets of neuronal dysfunction [6,7,22]. The present study follows from our recent observations implicating age-, inflammation- and experimental oxidative stress-induced PLA 2 activity in long-term memory (LTM) failure [7,22].…”

Section: Introductionmentioning

confidence: 98%

“…However, high levels of ROS are also shown to cause detrimental effects in the brain that negatively affect neuronal plasticity and memory function [3,4]. For instance, excessive (experimentally) increased levels of ROS are associated with decreased cognitive performance in both vertebrate and invertebrate species [1,2,4-7]. Under normal physiological conditions, the potential damaging effects of ROS is controlled by robust antioxidant defense systems.…”

Section: Introductionmentioning

confidence: 99%

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Oxidative-stress induced increase in circulating fatty acids does not contribute to phospholipase A2-dependent appetitive long-term memory failure in the pond snail Lymnaea stagnalis

et al. 2014

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BackgroundReactive oxygen species (ROS) are essential for normal physiological functioning of the brain. However, uncompensated increase in ROS levels may results in oxidative stress. Phospholipase A2 (PLA2) is one of the key players activated by elevated ROS levels resulting in the hydrolysis of various products from the plasmamembrane such as peroxidized fatty acids. Free fatty acids (FFAs) and fatty acid metabolites are often implicated to the genesis of cognitive impairment. Previously we have shown that age-, and experimentally induced oxidative stress causes PLA2-dependent long-term memory (LTM) failure in an aversive operant conditioning model in Lymnaea stagnalis. In the present study, we investigate the effects of experimentally induced oxidative stress and the role of elevated levels of circulating FFAs on LTM function using a non-aversive appetitive classical conditioning paradigm.ResultsWe show that intracoelomic injection of exogenous PLA2 or pro-oxidant induced PLA2 activation negatively affects LTM performance in our learning paradigm. In addition, we show that experimental induction of oxidative stress causes significant temporal changes in circulating FFA levels. Importantly, the time of training coincides with the peak of this change in lipid metabolism. However, intracoelomic injection with exogenous arachidonic acid, one of the main FFAs released by PLA2, does not affect LTM function. Moreover, sequestrating circulating FFAs with the aid of bovine serum albumin does not rescue pro-oxidant induced appetitive LTM failure.ConclusionsOur data substantiates previous evidence linking lipid peroxidation and PLA2 activation to age- and oxidative stress-related cognitive impairment, neuronal dysfunction and disease. In addition however, our data indicate that lipid peroxidation induced increased levels of circulating (per)oxidized FFAs are not a factor in oxidative stress induced LTM impairment.

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Section: Resultsmentioning

confidence: 54%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

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Oxidative-stress induced increase in circulating fatty acids does not contribute to phospholipase A2-dependent appetitive long-term memory failure in the pond snail Lymnaea stagnalis

et al. 2014

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Proteomic analysis of digestive tract peptidases and lipases from the invasive gastropod Pomacea canaliculata

Escobar‐Correas

Mendoza-Porras

Castro-Vázquez

et al. 2022

Pest Management Science

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Background The invasive gastropod Pomacea canaliculata has received great attention in the last decades as a result of its negative impact on crops agriculture, yet knowledge of their digestive physiology remains incomplete, particularly the enzymatic breakdown of macromolecules such as proteins and lipids. Results Discovery proteomics revealed aspartic peptidases, cysteine peptidases, serine peptidases, metallopeptidases and threonine peptidases, as well as acid and neutral lipases and phospholipases along the digestive tract of P. canaliculata. Peptides specific to peptidases (139) and lipases (14) were quantified by targeted mass spectrometry. Digestion begins in the mouth via diverse salivary peptidases (nine serine peptidases; seven cysteine peptidases, one aspartic peptidase and 22 metallopeptidases) and then continues in the oesophagus (crop) via three luminal metallopeptidases (Family M12) and six serine peptidases (Family S1). Downstream, the digestive gland provides a battery of enzymes composed of aspartic peptidase (one), cysteine peptidases (nine), serine peptidases (12) and metallopeptidases (24), including aminopeptidases, carboxypeptidases and dipeptidases). The coiled gut has M1 metallopeptidases that complete the digestion of small peptides. Lipid extracellular digestion is completed by triglyceride lipases. Conclusion From an integrative physiological and anatomical perspective, P. canaliculata shows an unexpected abundance and diversity of peptidases, which participate mainly in extracellular digestion. Moreover, the previously unknown occurrence of luminal lipases from the digestive gland is reported for the first time. Salivary and digestive glands were the main tissues involved in the synthesis and secretion of these enzymes, but plausibly the few luminally exclusive peptidases are secreted by ventrolateral pouches or epithelial unicellular glands. © 2022 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

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Lymnaea stagnalis as model for translational neuroscience research: From pond to bench

Rivi

Benatti

Colliva

et al. 2020

Neuroscience & Biobehavioral Reviews

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Phospholipase A2: The key to reversing long-term memory impairment in a gastropod model of aging

Cited by 12 publications

References 64 publications

Oxidative-stress induced increase in circulating fatty acids does not contribute to phospholipase A2-dependent appetitive long-term memory failure in the pond snail Lymnaea stagnalis

Oxidative-stress induced increase in circulating fatty acids does not contribute to phospholipase A2-dependent appetitive long-term memory failure in the pond snail Lymnaea stagnalis

Proteomic analysis of digestive tract peptidases and lipases from the invasive gastropod Pomacea canaliculata

Lymnaea stagnalis as model for translational neuroscience research: From pond to bench

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