Phospholipid analyses by MALDI-TOF/TOF mass spectrometry using 1,5-diaminonaphthalene as matrix

Wei, Dong; Shen, Qing; Baibado, Joewel Tarra; Liang, Yimin; Wang, Ping; Huang, Yi‐Hui Christine; Zhang, Zhifeng; Wang, Yixuan; Cheung, Hon-Yeung

doi:10.1016/j.ijms.2013.04.004

Cited by 25 publications

(28 citation statements)

References 34 publications

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“…This implies that both matrices are able to act as electron transfer agents to BChl. LDI data of DAN reported by Dong et al [31] evidenced Figure S1). f Homolytic cleavage with phytyl radical loss ( ) with the ensuing formation of a negatively charged ion at m/z 631.24.…”

Section: Matrix Evaluation For Bchl a Analysis By Maldi Msmentioning

confidence: 85%

Electron-Transfer Secondary Reaction Matrices for MALDI MS Analysis ofBacteriochlorophyll ainRhodobacter sphaeroidesand Its Zinc and Copper Analogue Pigments

Calvano

Ventura

Trotta

et al. 2016

J. Am. Soc. Mass Spectrom.

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Abstract. Bacteriochlorophyll a (BChl a), a photosynthetic pigment performing the same functions of chlorophylls in plants, features a bacteriochlorin macrocycle ring (18 π electrons) with two reduced pyrrole rings along with a hydrophobic terpenoid side chain (i.e., the phytol residue). Chlorophylls analysis by matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) is not so straightforward since pheophytinization (i.e., release of the central metal ion) and cleavage of the phytolester linkage are invariably observed by employing protonating matrices such as 2,5-dihydroxybenzoic acid, sinapinic acid, and α-cyano-4-hydroxycinnamic acid. Using BChl a from Rhodobacter sphaeroides R26 strain as a model system, different electron-transfer (ET) secondary reaction matrices, leading to the formation of almost ) ionization modes at m/z 910.55, were evaluated. Compared with ET matrices such as trans-2-[3-(4-t-butyl-phenyl)-2-methyl-2-propenylidene]malononitrile (DCTB), 2,2':5',2''-terthiophene (TER), anthracene (ANT), and 9,10-diphenylanthracene (DP-ANT), 1,5-diaminonaphthalene (DAN) was found to provide the highest ionization yield with a negligible fragmentation. DAN also displayed excellent ionization properties for two metal ion-substituted bacteriochlorophylls, (i.e., Znand Cu-BChl a at m/z 950.49 and 949.49), respectively. MALDI MS/MS of both radical charged molecular species provide complementary information, thus making analyte identification more straightforward.

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Section: Matrix Evaluation For Bchl a Analysis By Maldi Msmentioning

confidence: 85%

Electron-Transfer Secondary Reaction Matrices for MALDI MS Analysis ofBacteriochlorophyll ainRhodobacter sphaeroidesand Its Zinc and Copper Analogue Pigments

Calvano

Ventura

Trotta

et al. 2016

J. Am. Soc. Mass Spectrom.

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“…Approximately 300 mg of DAN was used (~0.16 mg/mm 2 ) and sublimated at 104°C and 50 mtorr for approximately 5 min. DAN has been shown to be an effective MALDI matrix for lipids (Dong et al, ). Sample slides were weighed before and after sublimation to ensure consistence between slides.…”

Section: Methodsmentioning

confidence: 99%

Two Specific Sulfatide Species Are Dysregulated during Renal Development in a Mouse Model of Alport Syndrome

Gessel

Spraggins

Voziyan

et al. 2019

Lipids

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Alport syndrome is caused by mutations in collagen IV that alter the morphology of renal glomerular basement membrane. Mutations result in proteinuria, tubulointerstitial fibrosis, and renal failure but the pathogenic mechanisms are not fully understood. Using imaging mass spectrometry, we aimed to determine whether the spatial and/or temporal patterns of renal lipids are perturbed during the development of Alport syndrome in the mouse model. Our results show that most sulfatides are present at similar levels in both the wild‐type (WT) and the Alport kidneys, with the exception of two specific sulfatide species, SulfoHex‐Cer(d18:2/24:0) and SulfoHex‐Cer(d18:2/16:0). In the Alport but not in WT kidneys, the levels of these species mirror the previously described abnormal laminin expression in Alport syndrome. The presence of these sulfatides in renal tubules but not in glomeruli suggests that this specific aberrant lipid pattern may be related to the development of tubulointerstitial fibrosis in Alport disease.

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“…Recently, matrices have been proposed for imaging lipids by mass spectrometry containing a combination of DHB with aniline, pyridine or 3-acetylpyridine, allowing analyses in both positive and negative ionization [37]. Also, Dong et al have reported on enhanced improvement in the analyses of phospholipids by MSI using 1,5-diaminonaphthalene as the matrix [38]. …”

Section: Methodological Advances In Sample Preparationmentioning

confidence: 99%

Current Status and Future Perspectives of Mass Spectrometry Imaging

Nimesh

Mohottalage

Vincent

et al. 2013

IJMS

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Mass spectrometry imaging is employed for mapping proteins, lipids and metabolites in biological tissues in a morphological context. Although initially developed as a tool for biomarker discovery by imaging the distribution of protein/peptide in tissue sections, the high sensitivity and molecular specificity of this technique have enabled its application to biomolecules, other than proteins, even in cells, latent finger prints and whole organisms. Relatively simple, with no requirement for labelling, homogenization, extraction or reconstitution, the technique has found a variety of applications in molecular biology, pathology, pharmacology and toxicology. By discriminating the spatial distribution of biomolecules in serial sections of tissues, biomarkers of lesions and the biological responses to stressors or diseases can be better understood in the context of structure and function. In this review, we have discussed the advances in the different aspects of mass spectrometry imaging processes, application towards different disciplines and relevance to the field of toxicology.

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Phospholipid analyses by MALDI-TOF/TOF mass spectrometry using 1,5-diaminonaphthalene as matrix

Cited by 25 publications

References 34 publications

Electron-Transfer Secondary Reaction Matrices for MALDI MS Analysis ofBacteriochlorophyll ainRhodobacter sphaeroidesand Its Zinc and Copper Analogue Pigments

Electron-Transfer Secondary Reaction Matrices for MALDI MS Analysis ofBacteriochlorophyll ainRhodobacter sphaeroidesand Its Zinc and Copper Analogue Pigments

Two Specific Sulfatide Species Are Dysregulated during Renal Development in a Mouse Model of Alport Syndrome

Current Status and Future Perspectives of Mass Spectrometry Imaging

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