Phospholipid based colloidal poloxamer–nanocubic vesicles for brain targeting via the nasal route

Salama, H. A.; Mahmoud, Azza A.; Kamel, Amany O.; Hady, Mayssa Abdel; Awad, Gehanne A.S.

doi:10.1016/j.colsurfb.2012.05.010

Cited by 106 publications

(38 citation statements)

References 51 publications

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“…1). It was reported by Salama et al that poloxamer 407 was able to alter the spherical shape of phospholipid-based colloidal nano-vesicles to cubic shape (32).…”

Section: Characterization Of Fluocinolone Acetonide Loaded Plga Nanopmentioning

confidence: 99%

A Novel Method for Preparing Surface-Modified Fluocinolone Acetonide Loaded PLGA Nanoparticles for Ocular Use: In Vitro and In Vivo Evaluations

2015

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ABSTRACT. Our objective was to prepare nanoparticulate system using a simple yet attractive innovated method as an ophthalmic delivery system for fluocinolone acetonide to improve its ocular bioavailability. Poly(lactic-co-glycolic acid) (PLGA) nanoparticles were prepared by adopting thin film hydration method using PLGA/poloxamer 407 in weight ratios of 1:5 and 1:10. PLGA was used in 75/25 and 50/50 copolymer molar ratio of DL-lactide/glycolide. Results revealed that using PLGA with lower glycolic acid monomer ratio exhibited high particle size (PS), zeta potential (ZP) and drug encapsulation efficiency (EE) values with slow drug release pattern. Also, doubling the drug concentration during nanoparticles preparation ameliorated its EE to reach almost 100%. Furthermore, studies for separating the un-entrapped drug in nanoparticles using centrifugation method at 20,000 rpm for 30 min showed that the separated clear supernatant contained nanoparticles encapsulating an important drug amount. Therefore, separation of un-entrapped drug was carried out by filtrating the preparation using 20-25 μm pore size filter paper to avoid drug loss. Aiming to increase the PLGA nanoparticles mucoadhesion ability, surface modification of selected formulation was done using different amount of stearylamine and chitosan HCl. Nanoparticles coated with 0.1% w/v chitosan HCl attained most suitable results of PS, ZP and EE values as well as high drug release properties. Transmission electron microphotographs illustrated the deposition of chitosan molecules on the nanoparticles surfaces. Pharmacokinetic studies on Albino rabbit's eyes using HPLC indicated that the prepared novel chitosan-coated PLGA nanoparticles subjected to separation by filtration showed rapid and extended drug delivery to the eye.

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“…1). It was reported by Salama et al that poloxamer 407 was able to alter the spherical shape of phospholipid-based colloidal nano-vesicles to cubic shape (32).…”

Section: Characterization Of Fluocinolone Acetonide Loaded Plga Nanopmentioning

confidence: 99%

A Novel Method for Preparing Surface-Modified Fluocinolone Acetonide Loaded PLGA Nanoparticles for Ocular Use: In Vitro and In Vivo Evaluations

2015

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“…In this work, the formulation strategy involved liposomes, due to their potential application for nose-brain delivery 20,21 and potential protection against enzymatic degradation. Chitosan, which has been well studied in the context of nasal delivery, [22][23][24] was the second excipient selected, due to its permeation-enhancing effect and mucoadhesive properties.…”

Section: Introductionmentioning

confidence: 99%

Development of coated liposomes loaded with ghrelin for nose-to-brain delivery for the treatment of cachexia

Salade¹,

Wauthoz²,

Deleu³

et al. 2017

IJN

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The aim of the present study was to develop a ghrelin-containing formulation based on liposomes coated with chitosan intended for nose–brain delivery for the treatment of cachexia. Among the three types of liposomes developed, anionic liposomes provided the best results in terms of encapsulation efficiency (56%) and enzymatic protection against trypsin (20.6% vs 0% for ghrelin alone) and carboxylesterase (81.6% vs 17.2% for ghrelin alone). Ghrelin presented both electrostatic and hydrophobic interactions with the anionic lipid bilayer, as demonstrated by isothermal titration calorimetry. Then, anionic liposomes were coated with N -(2-hydroxy) propyl-3-trimethyl ammonium chitosan chloride. The coating involved a size increment from 146.9±2.7 to 194±6.1 nm, for uncoated and coated liposomes, respectively. The ζ-potential was similarly increased from -0.3±1.2 mV to 6±0.4 mV before and after coating, respectively. Chitosan provided mucoadhesion, with an increase in mucin adsorption of 22.9%. Enhancement of permeation through the Calu3 epithelial monolayer was also observed with 10.8% of ghrelin recovered in the basal compartment in comparison to 0% for ghrelin alone. Finally, aerosols generated from two nasal devices (VP3 and SP270) intended for aqueous dispersion were characterized with either coated or uncoated liposomes. Contrarily to the SP270 device, VP3 device showed minor changes between coated and uncoated liposome aerosols, as shown by their median volume diameters of 38.4±5.76 and 37.6±5.74 µm, respectively. Overall, the results obtained in this study show that the developed formulation delivered by the VP3 device can be considered as a potential candidate for nose–brain delivery of ghrelin.

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“…Recent research on pluronics has generated new findings indicating immunomodulation and cytotoxicity-promoting properties of Poloxamer 407 revealing significant pharmacological interest. Human trials are in progress based on these results [ 57 ]. Along with these new findings and favourable properties, poloxamers have generated immense interest as one of the most promising novel mucoadhesive drug delivery systems.…”

Section: Poloxamer (Pluronics)mentioning

confidence: 99%

Novel Mucoadhesive Polymers for Nasal Drug Delivery

Anand¹,

Feridooni²,

Agu³

2012

Recent Advances in Novel Drug Carrier Systems

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Phospholipid based colloidal poloxamer–nanocubic vesicles for brain targeting via the nasal route

Cited by 106 publications

References 51 publications

A Novel Method for Preparing Surface-Modified Fluocinolone Acetonide Loaded PLGA Nanoparticles for Ocular Use: In Vitro and In Vivo Evaluations

A Novel Method for Preparing Surface-Modified Fluocinolone Acetonide Loaded PLGA Nanoparticles for Ocular Use: In Vitro and In Vivo Evaluations

Development of coated liposomes loaded with ghrelin for nose-to-brain delivery for the treatment of cachexia

Novel Mucoadhesive Polymers for Nasal Drug Delivery

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